Israel Ministry of Health

About: Israel Ministry of Health is a government organization based out in Jerusalem, Israel. It is known for research contribution in the topics: Population & Public health. The organization has 1633 authors who have published 2108 publications receiving 65387 citations.

Relationship between equipment and infrastructure for pandemic influenza and performance in an avian flu drill.

Abstract: Background: Effective preparedness for pandemic influenza necessitates acquisition and maintenance of vital equipment and infrastructure. The aim of this study was to investigate the relationship between the level of hospital preparedness relating to infrastructure and equipment and performance of the hospital in an avian flu drill. Methods: The levels of preparedness of the infrastructure and equipment for pandemic influenza of all 24 general hospitals were evaluated using a tool developed for this purpose. The hospital evaluation scores were then compared with the scores obtained by the hospitals in a simulated avian flu drill. Results: The overall scores of equipment and infrastructure for pandemic influenza of general hospitals ranged from 67% to 100%. Comparison of the overall level of preparedness of equipment and infrastructure for pandemic influenza with the overall scores achieved in the avian flu drill revealed a medium correlation. A medium correlation was also found between stockpiling of medications and performance in the avian flu drill. No correlations were found between operating infrastructure, availability of protective measures and medical forms and performance in the avian flu drill. Conclusions: This study has identified benchmarks of infrastructure and equipment required for managing a pandemic influenza event and evaluating the level of emergency preparedness of the hospital. The significant relationship between maintaining stockpiles of antiviral medications for patients and staff and performance in an avian flu drill emphasises its importance in the process of maintaining emergency preparedness for a pandemic influenza outbreak.

The Development of a Novel qPCR Assay-Set for Identifying Fecal Contamination Originating from Domestic Fowls and Waterfowl in Israel

Abstract: The emerging Microbial Source Tracking (MST) methodologies aim to identify fecal contamination originating from domestic and wild animals, and from humans. Avian MST is especially challenging, primarily because the Aves class includes both domesticated and wild species with highly diverse habitats and dietary characteristics. The quest for specific fecal bacterial MST markers can be difficult with respect to attaining sufficient assay sensitivity and specificity. The present study utilizes High Throughput Sequencing (HTS) to screen bacterial 16S rRNA genes from fecal samples collected from both domestic and wild avian species. Operational taxonomic unit (OTU) analysis was then performed, from which sequences were retained for downstream qPCR marker development. Identification of unique avian host DNA sequences, absent in non-avian hosts, was then carried out using a dedicated database of bacterial 16S rRNA gene taken from the Ribosomal Database Project. Six qPCR assays were developed targeting the 16S rRNA gene of Lactobacillus, Gallibacterium, Firmicutes, Fusobacteriaceae and other bacteria. Two assays (Av4143 and Av163) identified most of the avian fecal samples and demonstrated sensitivity values of 91% and 70%, respectively. The Av43 assay only identified droppings from battery hens and poultry, whereas each of the other three assays (Av24, Av13, and Av216) identified waterfowl species with lower sensitivities values. The development of an MST assay-panel, which includes both domestic and wild avian species, expands the currently known MST analysis capabilities for decoding fecal contamination.