Showing papers by "Mitsubishi published in 1978"

Separation and characterization of the outer membrane of Pseudomonas aeruginosa.

Abstract: A method is described for the preparation of outer and cytoplasmic membranes of Pseudomonas aeruginosa, and the outer membrane proteins characterized. Isolated outer and cytoplasmic membranes differed markedly in the content of 2-keto-3-deoxyoctonate (lipopolysaccharide) and phospholipid as well as in the localization of certain enzymes (NADH oxidase, succinate dehydrogenase, D-lactate dehydrogenase, malate dehydrogenase, and phospholipase), and also in the microscopic morphology. The outer membrane preparation showed activity neutralizing a certain bacteriocin or bacteriophages, whereas the cytoplasmic membrane preparation showed no neutralizing activity. The protein composition of membrane preparations from five different strains of P. aeruginosa [P14, M92 (PAO1), PAC1, P15, and M2008 (PAT)] were determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. More than 50 protein bands were detected in the cytoplasmic membrane preparation. The protein compositions of outer membranes from the five different strains were very similar: at least 6 major bands were found (apparent molecular weights: Band D, 50,000; band E, 45,000; band F, 33,000; bands G and H, 21,000; and band I, 8,000). The protein composition of outer membranes was affected by some physiological growth conditions. Some features of major outer membrane proteins were also studied. Band F showed anomalous migration on SDS polyacrylamide gel electrophoresis depending on the solubilizing conditions or pretreatment with TCA. Band I seemed to be a protein analogous to the lipoprotein which had been found in the outer membrane of Escherichia coli.

MOS Semiconductor device

Abstract: The disclosed MOS transistor includes a channel region formed of a lightly doped semiconductor layer disposed in a surface portion of a heavily doped semiconductor layer subsequently disposed on a lightly doped semiconductor substrate. The channel region may be of the identical or opposite conductivity type to the heavily doped semiconductor layer that has the same type conductivity as the substrate. Also the channel region may be of an intrinsic semiconductive material. A source and a drain region may be disposed in the lightly or highly doped layer. Alternatively the source and drain regions may reach the substrate.

Coating composition and a method for producing a synthetic resin molded product having an abrasion resistant surface

Abstract: This invention relates to a coating composition which comprises a polyfunctional monomer having at least three acryloyloxy groups and/or methacryloyloxy groups in one molecule, a monomer having not more than two acryloyloxy group and/or methacryloyloxy groups in one molecule and optionally a photosensitizer and which can form a crosslink-hardened film excellent in abrasion resistance upon curing by irradiation with actinic radiation in air and a method for producing a synthetic resin molded product having an abrasion resistant surface.

Method and apparatus for the measurement of antigens and antibodies

Abstract: A method of the quantitative measurement of antigens and antibodies by reacting antibody- or antigen-sensitized insoluble carrier particles with a corresponding antigen or antibody or a mixture thereof in a sample, irradiating the reaction mixture with light of a specific wavelength to measure the transmitted light at two or more points of time as the reaction proceeds, and then evaluating an increase in absorbance or percent absorption of the reaction mixture for a given period of time, and an apparatus for use therein.

High-viscosity refrigerator oil compositions

Abstract: A high-viscosity refrigerator oil composition comprising (A) a polyglycol oil such as a polyoxypropylene glycol or an alkyl ether thereof and (B) 0.1-10% by weight, based on the total weight of (A) and (B), of a glycidyl ether type epoxy compound, an epoxidized fatty acid monoester and/or an epoxidized vegetable oil.

Process and apparatus for waste water treatment

Abstract: Waste water containing organic pollutants is purified by biological treatment with aerobic microorganisms supported on hollow fibers having microporous walls through which oxygen is supplied to the microorganisms.

Studies on Polypeptide‐Chain‐Elongation Factors from an Extreme Thermophile, Thermus thermophilus HB8

Abstract: Catalytic properties of the elongation factors from Thermus thermophilus HB8 have been studied and compared with those of the factors from Escherichia coli. 1 The formation of a ternary guanine-nucleotide · EF-Tu · EF-Ts complex was demonstrated by gel filtration of the T. thermophilus EF-Tu · EF-Ts complex on a Sephadex G-150 column equilibrated with guanine nucleotide. The occurrence of this type of complex has not yet been proved with the factors from E. coli. 2 The dissociation constants for the complexes of T. thermophilus EF-Tu · EF-Ts with GDP and GTP were 6.1 × 10−7 M and 1.9 × 10−6 M respectively. On the other hand, T. thermophilus EF-Tu interacted with GDP and GTP with dissociation constants of 1.1 × 10−9 M and 5.8 × 10−8 M respectively. This suggests that the association of EF-Ts with EF-Tu lowered the affinity of EF-Tu for GDP by a factor of about 600 and facilitated the nucleotide exchange reaction. 3 Although the T. thermophilus EF-Tu · EF-Ts complex hardly dissociates into EF-Tu and EF-Ts, a rapid exchange was observed between free EF-Ts and the EF-Tu · EF-Ts complex using 3H-labelled EF-Ts. The exchange reaction was independent on the presence or absence of guanine nucleotides. 4 Based on the above findings, an improved reaction mechanism for the regeneration of EF-Tu GTP from EF-Tu · GDP is proposed. 5 Studies on the functional interchangeability of EF-Tu and EF-Ts between T. thermophilus and E. coli has revealed that the factors function much more efficiently in the homologous than in the heterologous combination. 6 T. thermophilus EF-Ts could bind E. coli EF-Tu to form an EF-Tu (E. coli) · EF-Ts (T. thermophilus) hybrid complex. The complex was found to exist in a dimeric form indicating that the property to form a dimer is attributable to T. thermophilus EF-Ts. On the other hand, no stable complex between E. coli EF-Ts and T. thermophilus EF-Tu has been isolated. 7 The uncoupled GTPase activity of T. thermophilus EF-G was much lower than that of E. coli EF-G. T. thermophilus EF-G formed a relatively stable binary EF-G · GDP complex, which could be isolated on a nitrocellulose membrane filter. The Kd values for EF-G · GDP and EF-G · GTP were 6.7 × 10−7 M and 1.2 × 10−5 M respectively. The ternary T. thermophilus EF-G · GDP · ribosome complex was again very stable and could be isolated in the absence of fusidic acid. The stability of the latter complex is probably the cause of the low uncoupled GTPase activity of T. thermophilus EF-G.

Inductor type synchronous motor driving system for minute control of the position and the rotation angle of the motor

Abstract: A system for driving an inductor type synchronous motor for minute control thereof which includes an inductor type synchronous motor having a circuit for driving m phases of driving windings and first magnetic teeth. A second group of magnetic teeth is provided facing the first group of magnetic teeth. Apparatus is provided for feeding electric current from a DC power source to the driving windings of the respective phases. The driving winding for one phase intersects the flux of a partial set of first magnetic teeth or at least two magnetic teeth to provide a flux-interlink. A group of solid state switches are interposed between the source for producing the electric current and the driving windings of the respective phases. A distribution pattern generator generates patterns in response to a command signal and controls the on-off states of the solid-state switches with the noted patterns to thereby form the electric current into trigonometric-functional-patterns. The minute control characterized with an electrical resolution number R, per electrical angle 2π (2π being one fundamental cycle) of the apparatus for feeding electric current, is equal to or larger than 3m per electrical cycle. The minute control is thereby determined to be stepless.

Synthetic papers and method of making the same

Abstract: A laminated structure forming a synthetic paper made of a base or backing film layer made of a thermoplastic resin in a biaxially oriented state. A paper-like film layer is laminated to the base film layer and comprises a thermoplastic resin having 0.5 to 65% by weight of particles of a fine, inorganic filler dispersed in the resin thereof substantially uniformly. The paper-like film layer is in an uniaxially oriented state and has microvoids dispersed therein formed by stretching the laminated films in a heated state. The microvoids comprise microvoids open to a surface of the paper-like film layer free of the base film layer and include microvoids, open and closed, distributed in the paper-like film layer defining therein a cellular, fibrous structure. The two films are formed from thermoplastic resinous compositions in which the components of each composition are well admixed and particles of a fine filler are uniformly distributed in the thermoplastic resin of the paper-like film layer to obtain, as much as possible, microvoids distributed therein uniformly. After the base film layer is formed, it is stretched in a heated state in a machine for longitudinal direction to orient the film uniaxially. The paper-like layer is laminated under pressure on one surface of the base film layer while in a melted state and the resulting laminated structure is stretched transversely to at least 2.5 times the original transverse dimension of the portion of film being stretched between grippers of a stretching machine. This orients the base film layer biaxially and the paper-like film layer uniaxially. The stretching must be sufficient for the stretching and particles of filler to develop microvoids in the paper-like film layer rendering it a cellular and fibrous structure.

Cloning of Bacillus subtilis leucine A, B and C genes with Escherichia coli plasmids and expression of the leuC gene in E. coli

Abstract: The leucine genes of Bacillus subtilis have been cloned directly from the chromosomal DNA into Escherichia coli leuB cells by selection for the Leu+ phenotype using RSF2124 as a vector plasmid. The hybrid plasmid designated RSF2124-B·leu contained a 4.2 megadalton fragment derived from B. subtilis DNA, including the leu genes. The fragment had one site susceptible to EcoRI* and another site susceptible to BamNI endonuclease. Among the three fragments produced by EcoRI* and BamNI endonucleases, the 1.2 megadalton fragment had the ability to transform B. subtilis leuA, leuB and leuC auxotrophs to leu+. However, B. subtilis ilvB and ilvC auxotrophs were not rescued even by the whole 4.2 megadalton fragment present in the hybrid plasmid. β-Isopropylmalate dehydrogenase (leuB gene product) activity found in E. coli cells containing the hybrid plasmid was about 60% of that in E. coli wild type cells, despite the high copy number (7.8) of the plasmid per chromosome observed.

Method and apparatus for the measurement of glucose content

Abstract: A method and apparatus for measuring the glucose content in a liquid sample by passing the sample consecutively through a column of at least one ion exchange resin and a column of immobilized glucose oxidase and then determining the amount of hydrogen peroxide produced by contacting the sample with glucose oxidase.

Genetic analysis of products of protoplast fusion in saccharomyces cerevisiae

Abstract: Protoplasts of Saccharomyces cerevisiae were prepared from two different haploid strains both of mating type a, which carried different nuclear (ade1, ura1, his4, leu2 and thr4) and mitochondrial (ρ, ω, CR, ER and OR) markers, and were fused with the aid of polyethylene glycol. Cells of fused products (prototrophs) displayed phenotype of mating type a and were crossed to mating type α/α diploids having auxotrophic markers, e.g. trp1. On sporulation of the resulting hybrid clones, as a rule, there were three tetrad types for mating types, i.e. (I) 4non-maters, (II) 2a:2α and (III) a:α: 2non-maters. The relative frequencies of these three tetrad types were close to the ones theoretically predicted from a/a/α/α tetraploids, suggesting that the fusion products were a/a diploids. Auxotrophic markers involved in these crosses, which were located on four different chromosomes, were also segregated to yield the tetrad distributions expected from the parentages. Consequently, it was concluded that the protoplast fusion proceeded to karyogamy to produce stable diploids. A study of mitochondrial recombination demonstrated that the fusion products accepted the mitochondrial genome (the polar gene ω as well as the drug resistance genes) from one parent of ρ+, but not from another of neutral petite.

Determination of water adsorption sites in wood by a hydrogen–deuterium exchange

Abstract: IT is important in the field of forest products science to know the sorption mechanism of water in wood Adsorption of water onto hygroscopic natural polymers, such as wood and cellulose, is thought to depend entirely on the hydroxyl groups on the surface1 The mechanism of water adsorption in wood can therefore be studied by evaluating the quantity and the state of the adsorption sites, hydroxyl groups Until now, in order to measure the number of adsorption sites of water in wood, it has been usual to draw a sorption isotherm from which the adsorbed area can be calculated assuming monomolecular layer adsorption according to the BET theory2 Little is known of the sorption mechanism on the molecular level, however We report here the use of a hydrogen–deuterium exchange method to determine the adsorption sites of water in wood Hydrogen–deuterium exchange is a well established method which has been widely used to determine hydroxyl group accessibility3,8,9

Method of producing of treating material for oil-containing waste water

Abstract: An oil-adsorbing composition useful for removing oil from oil-contaminated water comprises a granulated mixture of 5-80 weight % of an inorganic filler capable of adsorbing oil and 95-20 weight % of a cross-linked polymer. The composition is melt-kneaded by mixing and crushing the ingredients while cross-linking the polymer.

Lid device for use in a motorcar

Abstract: An opening/closing device for a lid adapted to openably cover an opening of, for example, a trunk of a motorcar does not substantially project into the trunk when the lid is closed. An elongated guide slot extends along a side edge of the opening. A first arm member has one end fixedly secured to the lid and the other end slidably fitted in the guide slot. A second arm member has one end pivotably mounted on the first arm member or on the lid and the other end pivotably mounted on the side edge of the opening. The first and second arm members are adapted to be folded into positions substantially parallel to each other when the lid is closed.

Benzaldehyde as a Carcinostatic Principle in Figs

Abstract: (1978). Benzaldehyde as a Carcinostatic Principle in Figs. Agricultural and Biological Chemistry: Vol. 42, No. 7, pp. 1449-1451.

Comparison of conformation and antimicrobial activity of synthetic analogs of gramicidin S: Stereochemical consideration of the role of D-phenylalanine in the antibiotic.

Abstract: In order to study the role of D-amino acid residues in keeping the stable β-sheet conformation and in the antimicrobial activity of gramicidin S (GS), the four analogs of GS containing D-Ala, L-Ala, Gly, and Aib (α-aminoisobutyric acid) in place of D-Phe were synthesized. D-Ala-and Gly-containing analogs showed antimicrobial activity, while those containing L-Ala and Aib showed no activity. Conformation of these analogs and their derivatives were studied by comparison of ORD and CD spectra and by slective methylation method. It is concluded that the biologically active analogs have β-sheet conformation while inactive analogs have a much different conformation from that of GS. This indicates that D-Ala-Pro and Gly-Pro sequences favor taking a β-bend form but L-Ala-Pro and Aib-Pro sequences do not because the presence of L-side methyl group on the α-carbon atom of LAla and Aib residues destabilizes the β-bend form. This would explain why the inactive analogs which take a different conformation from that of the active ones result in the loss of activity.

Sheet material and method of producing the same

Abstract: A sheet material is formed of a base cloth consisting of a woven fabric, knit fabric, nonwoven fabric, or web material having a favorable form stablity and a short fiber material consisting at least partly of a natural fiber material, said sheet material having a structure in which said base cloth is covered on at least one surface with the short fiber material and a part of the short fiber material is stuffed into the gaps in the network of the base cloth so that the base cloth and short fiber material are substantially integrated with each other.

Construction of a recombinant plasmid composed of B. subtilis leucine genes and a B. subtilis (natto) plasmid: its use as cloning vehicle in B. subtilis 168.

Abstract: Recombinant plasmids composed of Bacillus subtilis 168 leucine genes and a B. subtilis (natto) plasmid have been constructed in a recombination deficient (recE4) mutant of Bacillus subtilis 168. The process involved EcoRI fragmentation and ligation of a B. subtilis (natto) plasmid and a composite plasmid RSF2124-B · leu in which B. subtilis 168 leucine genes are linked to the R-factor RSF2124. A constructed plasmid (pLS102) was found to be composed of an EcoRI fragment derived from the vector plasmid and two tandemly repeated EcoRI fragments carrying the leucine genes. A derivative plasmid (pLS101 or pLS103) consisting of one molecule each of the EcoRI fragments was obtained by in vivo intramolecular recombination between the repeated leucine gene fragments in pLS102. pLS103 was cleaved once with BamNI, SmaI and HpaI. Insertion of foreign DNA (Escherichia coli plasmid pBR322) into the BamNI site inactivated leuA but not the leuC function which thus can serve as selective marker if the plasmid is used as vector in molecular cloning. The penicillin resistance carried in pBR322 was not functionally expressed in B. subtilis cells. By partial digestion of pLS103 with HindIII followed by ligation with T4-induced ligase, pLS107 was obtained which contained only one EcoRI site. However, insertion of exogenous DNA (pBR322) into this EcoRI site inactivated both leuA and leuC functions.

Outer panel construction for a vehicle or the like

Abstract: The present invention is directed at a novel side outer panel construction which includes a front panel having a front door opening and a first center pillar forming portion in its rear end. The present invention further includes a rear panel having a rear door opening and a second center pillar forming portion in its front end.

Polyolefin resin composition and method for making polyolefin film

Abstract: A polyolefin resin composition obtained by mixing a polyolefin resin with minor proportion of a polybutene of low molecular weight, a mixed glyceride containing a lower acyl group and a higher acyl group, and preferably a surface wetting agent, is used to form a polyolefin film, which is thereafter aged. The thus formed film exhibits very excellent adhesion strength as well as very good transparency and physical strength, and is therefore suitable for use as a food packaging film.

Process for controlling nitrogen oxides in exhaust gases

Abstract: A process for controlling nitrogen oxides in combustion exhaust gases which comprises adding an oxygen-containing hydrocarbon and/or its precursor to exhaust gases and thereby oxidizing nitric oxide in the exhaust to nitrogen dioxide in the presence of oxygen.

PCM Recording and reproducing system

Abstract: A reproduced PCM signal in the form of continuous frames is subjected to a code check after the separation of its frame synchronizing signals and then stored in a memory. If more than two consecutive frames are determined to include code errors resulting from a spliced portion of the associated PCM magnetic tape than those frames are prevented from being written into the memory but the latter is continuously read out by skipping the lost data and reading other data stored in the memory. Also the amount of data stored in the memory is sensed and maintained at a predetermined constant magnitude by controlling the speed of travel of the tape.

A process for the diffusion welding of copper and stainless steel

Abstract: A process for the diffusion welding of copper and stainless steel, which comprises sandwiching a thin layer of at least one metal selected from the group consisting of Ni, Ni-base alloys, Cr, Ni-Cr, and Cr-Ni with little gas contents, as an insert metal, between the surfaces of copper and stainless steel to be bonded, and then diffusion welding the sandwich.