Showing papers by "Scottish Association for Marine Science published in 2005"

Monitoring environmental variability around cold-water coral reefs: the use of a benthic photolander and the potential of seafloor observatories

Abstract: The environmental sensitivies of cold-water corals and their associated biota are likely to be determined by the natural variability of the cold-water coral reef environment. The sensitivity of reef biota to sedimentation and resuspension events is largely unknown and the influence of seasonal phytodetrital deposition is poorly understood. Here we describe the use of a benthic photolander to monitor this variability by the Sula Ridge reef complex on the mid-Norwegian continental shelf and from the Galway carbonate mound in the Porcupine Seabight. The photolander provides a platform for time-lapse digital and film cameras to image the seabed while recording the current regime and optical characteristics (light transmission, backscatter and fluorescence) of the seawater. In its first two deployments carried out in 2001 and 2002 by the Sula Ridge the lander recorded a dynamic environment around the reef site with a tidal current regime and periods of sediment resuspension. Current speeds by the Sula Ridge reef complex reached a maximum of 28 cm s−1 and 70 cm s−1 on the Galway carbonate mound, reinforcing much speculation about the dependence of these communities on current-swept conditions. Seabed photographs show intense feeding activity of echiuran worms (Bonellia viridis) near the Sula Ridge reef complex pointing to rapid bioturbation of the sediment. Fish were recorded sheltering near sponges that had colonised glacial dropstones. Longer term monitoring in situ is needed for study of seasonal change, to identify functional roles of associated fauna and to monitor potential coral spawning events. Benthic landers and seafloor observatories have great potential in these areas. Only with a better understanding of the natural variability of the cold-water coral environment can informed decisions about the environmental sensitivity of cold-water coral reefs and their management be made.

Water mass modification in an Arctic fjord through cross-shelf exchange: The seasonal hydrography of Kongsfjorden, Svalbard

Abstract: [1] Kongsfjorden and the West Spitsbergen Shelf is a region whose seasonal hydrography is dominated by the balance of Atlantic Water, Arctic waters, and glacial melt. Regional seasonality and the cross-shelf exchange processes have been investigated using conductivity-temperature-depth (CTD) observations from 2000–2003 and a 5-month mooring deployment through the spring and summer of 2002. Modeling of shelf-fjord dynamics was performed with the Bergen Ocean Model. Observations show a rapid and overwhelming intrusion of Atlantic Water across the shelf and into the fjord during midsummer giving rise to intense seasonality. Pockets of Atlantic Water, from the West Spitsbergen Current, form through barotropic instabilities at the shelf front. These leak onto the shelf and propagate as topographically steered features toward the fjord. Model results indicate that such cross-front exchange is enhanced by north winds. Normally, Atlantic Water penetration into the fjord is inhibited by a density front at the fjord mouth. This geostrophic control mechanism is found to be more important than the hydraulic control common to many fjords. Slow modification of the fjord water during spring reduces the effectiveness of geostrophic control, and by midsummer, Atlantic Water intrudes into the fjord, switching from being Arctic dominant to Atlantic dominant. Atlantic Water continues to intrude throughout the summer and by September reaches some quasi steady state condition. The fjord adopts a ‘‘cold’’ or ‘‘warm’’ mode according to the degree of Atlantic Water occupation. Horizontal exchange across the shelf may be an important process causing seasonal variability in the northward heat transport to the Arctic.

Habitat-specific normal and reverse diel vertical migration in the plankton-feeding basking shark

Abstract: Summary 1. Megaplanktivores such as filter-feeding sharks and baleen whales are at the apex of a short food chain (phytoplankton‐zooplankton‐vertebrate) and are sensitive indicators of sea-surface plankton availability. Even though they spend the majority of their time below the surface it is still not known how most of these species utilize vertical habitat and adapt to short-term changes in food availability. 2. A key factor likely to control vertical habitat selection by planktivorous sharks is the diel vertical migration (DVM) of zooplankton; however, no study has determined whether specific ocean-habitat type influences their behavioural strategy. Based on the first high-resolution dive data collected for a plankton-feeding fish species we show that DVM patterns of the basking shark Cetorhinus maximus reflect habitat type and zooplankton behaviour. 3. In deep, well-stratified waters sharks exhibited normal DVM (dusk ascent‐dawn descent) by tracking migrating sound-scattering layers characterized by Calanus and euphausiids. Sharks occupying shallow, inner-shelf areas near thermal fronts conducted reverse DVM (dusk descent‐dawn ascent) possibly due to zooplankton predator‐prey interactions that resulted in reverse DVM of Calanus . 4. These opposite DVM patterns resulted in the probability of daytime-surface sighting differing between these habitats by as much as two orders of magnitude. Ship-borne surveys undertaken at the same time as trackings reflected these behavioural differences. 5. The tendency of basking sharks to feed or rest for long periods at the surface has made them vulnerable to harpoon fisheries. Ship-borne and aerial surveys also use surface occurrence to assess distribution and abundance for conservation purposes. Our study indicates that without bias reduction for habitat-specific DVM patterns, current surveys could under- or overestimate shark abundance by at least 10-fold.

Regeneration from Injury and Resource Allocation in Sponges and Corals – a Review

Abstract: The ability of bottom-dwelling marine epifauna to regenerate injured or lost body parts is critical to the survival of individuals from disturbances that inflict wounds. Numerous studies on marine sponges (Phlyum Porifera) and corals (of the orders Scleractinia and Alcyonacea) suggest that regeneration is limited by many intrinsic (individual-dependent) and extrinsic (environment-dependent) factors, and that other life history processes may compete with regeneration for energetic and cellular resources. We review how intrinsic (size, age, morphology, genotype) and extrinsic (wound characteristics, water temperature, food availability, sedimentation, disturbance history, selection) factors limit regeneration in sponges and corals. We then review the evidence for impaired somatic growth and sexual reproduction, and altered outcomes of interactions (anti-predator defenses, competitive abilities, self- and non-self recognition abilities) with other organisms in regenerating sponges and corals. We demonstrate that smaller, older sponges and corals of decreasing morphological complexities tend to regenerate less well than others, and that regeneration can be modulated by genotype. Large wounds with small perimeters inflicted away from areas where resources are located tend to be regenerated less well than others, as are injuries inflicted when food is limited and when the animal has been previously or recently injured. We also demonstrate that regeneration strongly impairs somatic growth, reduces aspects of sexual reproduction, and decreases the ability for sponges and corals to defend themselves against predators, to compete, and to recognize conspecifics. Effects of limited regeneration and impaired life histories may manifest themselves in higher levels of biological assembly e.g., reduced accretion of epifaunal biomass, reduced recruitment and altered biotic associations, and thus affect marine community and ecosystem recovery from disturbances.

Adaptations of amphibious fish for surviving life out of water

Abstract: There are a small number of fish species, both marine and freshwater, that exhibit a truly amphibious habit that includes periods of aerial exposure. The duration of emersion is reflected in the level of physical and physiological adaptation to an amphibious lifestyle. Fish that are only briefly out of water retain predominantly aquatic attributes whereas there are semi-terrestrial species that are highly adapted to prolonged periods in the aerial habitat. Desiccation is the main stressor for amphibious fish and it cannot be prevented by physiological means. Instead, amphibious fish resist excessive water loss by means of cutaneous modification and behavioural response. The more terrestrially adapted fish species can tolerate considerable water loss and may employ evaporation to aid thermoregulation. The amphibious habit is limited to fish species that can respire aerially. Aerial respiration is usually achieved through modification to existing aquatic pathways. Freshwater air-breathers may respire via the skin or gills but some also have specialized branchial diverticula. Marine species utilize a range of adaptations that may include modified gills, specialized buccopharyngeal epithelia, the intestine and the skin. Areas of enhanced respiratory activity are typified by increased vascularization that permits enhanced perfusion during aerial exposure. As with other adaptations the mode of nitrogenous elimination is related to the typical durations of emersion experienced by the fish. Intertidal species exposed on a regular cycle, and which may retain some contact with water, tend to remain ammoniotelic while reducing excretion rates in order to prevent excessive water loss. Amphibious fish that inhabit environments where emersion is less predictable than the intertidal, can store nitrogen during the state of emersion with some conversion to ureotelism or have been shown to tolerate high ammonia levels in the blood. Finally, the more amphibious fish are more adapted to moving on land and seeing in air. Structural modifications to the pectoral, pelvic, dorsal and anal fins, combined with a well-developed musculature permit effective support and movement on land. For vision in air, there is a general trend for fish to possess close-set, moveable, protruberant eyes set high on the head with various physical adaptations to the structure of the eye to allow for accurate vision in both air and water.

Lipids and nitrogen isotopes of two deep-water corals from the North-East Atlantic: initial results and implications for their nutrition

Abstract: The lipid and organic nitrogen isotopic (δ15N) compositions of two common deep-water corals (Lophelia pertusa and Madrepora oculata) collected from selected locations of the NE Atlantic are compared to the composition of suspended particulate organic matter, in order to determine their principle food source. Initial results suggest that they may feed primarily on zooplankton. This is based on the increased abundances of mono-unsaturated fatty acids and alcohols and the different ratios of the polyunsaturated fatty acids, 22:6/20:5 of the corals when compared to those of the suspended particulate organic matter. There is enrichment in L. pertusa of mono-unsaturated fatty acids and of δ15N relative to M. oculata. It is unclear whether this reflects different feeding strategies or assimilation/storage efficiencies of zooplankton tissue or different metabolism in the two coral species.

Iodine and Halocarbon Response of Laminaria digitata to Oxidative Stress and Links to Atmospheric New Particle Production

Abstract: Environmental Context.Various organic iodine compounds (including CH3I, CH2ClI, CH2BrI, CH2I2) are present throughout the marine boundary layer as a result of their production from seaweeds, phytoplankton, and photolysis reactions occurring in seawater. In air, these compounds rapidly photolyse to give atomic I which subsequently reacts with ozone to form iodine oxide, potentially leading to perturbations of the tropospheric oxidative capacity and nucleation of atmospheric particles. Recent research has identified molecular iodine as an additional source of iodine atoms to coastal areas. Here we study the relative roles and controls of gaseous organic and molecular iodine release from the seaweed Laminaria digitata. Abstract.Changes in the halocarbon, I2 and particle production of the brown algal kelp Laminaria digitata as a response to different chemical stresses have been investigated. Oxidative stress (caused by either exogenous hydrogen peroxide, gaseous ozone or a solution of oligoguluronates, known elicitors of oxidative stress) caused increased halocarbon and I2 production by the seaweed. The maximum I2 release was observed under exposure to O3 (at several hundred parts per billion by volume (ppbv)), whereas oligoguluronates elicited the highest release of iodine-containing halocarbons including CH2I2. Significantly greater production of I2, compared to CH2I2, was observed at atmospheric levels of ozone. Particle production was observed only when the Laminaria samples were exposed to ozone (up to 16 000 cm-3 s-1 per gram fresh weight (FW) of seaweed with a ~2 min residence time and with a total I atom flux of 1.6 × 108 cm-3 s-1 g-1 FW from photolysis of I2); passing O3-free air over the unstressed seaweed followed by secondary mixing with ozone did not result in any measurable particle formation. Our limited data indicate that ozone elicits abiotic production of I2 from Laminaria and that there is a direct relationship between the amount of I2 released and the number of particles formed. The results support the recent hypothesis that molecular iodine rather than volatile organic iodine (e.g. CH2I2) release from exposed seaweeds is the major source of coastal new particle production.

Effect of external pH on the growth, photosynthesis and photosynthetic electron transport of Chlamydomonas acidophila Negoro, isolated from an extremely acidic lake (pH 2.6)

Abstract: In extremely acidic lakes, low primary production rates have been measured. We assumed that proton stress might explain these observations and therefore investigated the photosynthetic behaviour of a Chlamydomonas species, a main primary producer in acidic lakes, over a range of pH values. Identified as C. acidophila using small subunit rDNA analysis, this species is identical to other isolates from acidic environments in Europe and South America, suggesting a worldwide distribution. Laboratory experiments with C. acidophila, revealed a broad pH-tolerance for growth and photosynthesis, the lower pH limit lying at pH 1.5 and the upper limit at pH 7. Growth rates at optimum pH conditions (pH 3 and 5) were equal to those of the mesophilic Chlamydomonas reinhardtii. In contrast, photosynthetic rates were significantly higher, suggesting that higher photosynthetic rates compensated for higher dark respiration rates, as confirmed experimentally. Electron transport capacities of PSI and PSII, P700 + re-reduction times and measurements of PSII fluorescence revealed the induction of alternative electron transport mechanisms, such as chlororespiration, state transitions and cyclic electron transport, only at suboptimal pH values (pH 1.5; 4 and 6-7). The results indicate, that C. acidophila is well adapted to low pH and that the relatively low primary production rates are not a result of pH stress.

A mathematical model of the growth of sea lice, Lepeophtheirus salmonis, populations on farmed Atlantic salmon, Salmo salar L., in Scotland and its use in the assessment of treatment strategies

Abstract: Sea lice are a persistent problem for farmed and wild salmonid populations. Control can be achieved through the use of veterinary medicines. A model was developed to describe the patterns of sea lice infection on salmon farms in Scotland and to predict the likely effect of various treatment strategies. This model takes into account development rates and mortality using compartments representing life history stages and external infection pressure. The national sea lice infection pattern was described using parameters representing stage survival, background infection levels and egg viability rates. The patterns observed across farms varied greatly and the model gave broad agreement to observed trends with different parameters being required in the model for sites using hydrogen peroxide and cypermethrin treatments. The parameter estimates suggest that the background infection pressure on sites where cypermethrin was administered was higher than for those using hydrogen peroxide. Both models had comparable magnitudes of sensitivity with survival from one stage to another being the most sensitive parameter, followed by feedback rates at which gravid females produce eggs, with background infection levels the least sensitive. The effect of different cypermethrin treatment strategies was assessed using the model. Increasing treatments in a production cycle gave more effective control. However, the model showed that timing of treatments is most important if sea lice are to be effectively controlled.

Distinction between multiple isolates of chlorella vulgaris (chlorophyta, trebouxiophyceae) and testing for conspecificity using amplified fragment length polymorphism and its rdna sequences†

Abstract: Multiple strains of individual algal species are available from public culture collections, often with the same isolate being maintained in parallel at a number of collections under different culture regimes. To unravel genomic variation and to identify unique genotypes among such multiple strains, two approaches were used on a sample of 29 strains of Chlorella vulgaris Beijerinck, an alga of great value for applied research, from five culture collections. With the exception of two strains, internal transcribed spacer rDNA sequence data substantiated conspecificity of the studied strains and only minor sequence differences with the authentic "Beijerinck isolate" were observed. Amplified fragment length polymorphism (AFLP) detected considerable genomic variation when rDNA sequences were identical. Band detection and the construction of a binary matrix from AFLP patterns for phylogenetic analyses were fully automated, but comparison of similar patterns still required manual refinement. The AFLPs distinguished 11 unique genotypes and provided robust support for the presence of five cryptic species. This finding advocates the need to carefully record which strain has been used in any experiment or in applied research, because genomic variation may also correspond to differences in physiological/biochemical properties. No genomic differences could be detected between duplicate strains of the same isolate that were maintained by continuous subculturing over many decades or within those stored at ultralow temperatures.

Echinoderms: Their Culture and Bioactive Compounds

Abstract: Of the five extant classes of echinoderms, it is the sea urchins (Echinoidea) and the sea cucumbers (Holothuroidea) that are both commercially fished and heavily overexploited. In sea urchins, it is the gonad of both males and females, normally referred to as ‘roe’, that is a sought-after food. In the sea cucumber, the principal product is the boiled and dried body-wall or ‘beche-de-mer’ for which there is an increasing demand. Many sea urchin and sea cucumber fisheries still have no management system or restrictions in place, and for those that do, the prognosis for catches to continue even at a reduced level is poor. Cultivation of these species increasingly becomes a necessity, both for stock enhancement programs and as a means to meet market demand. Sea urchin culture has been practised on a large scale in Japan for many decades, and effective methods for the culture and reseeding of species in these waters have been long established. Juvenile urchins are produced in their millions in state-sponsored hatcheries, for release to managed areas of seafloor. Outside of Japan, sea urchin cultivation is still a fairly recent practice, less than 10 years old, and largely still at a research level, although a range of species are now being produced in a variety of different culture systems. It is essential that the culture systems are adapted to be species-specific and meet with local environmental constraints. Sea cucumber cultivation originated in Japan in the 1930s and is now well established there and in China. Methods for mass cultivation of the tropical Holothuria scabra are now well established and practised in India, Australia, Indonesia, the Maldives and the Solomon Islands, with the focus of the research effort for both temperate and tropical species being centred on the production of juveniles in hatcheries for the restoration and enhancement of wild stocks. Like many other marine organisms, echinoderms have been, and continue to be, examined as a source of biologically active compounds with biomedical applications. Sea cucumber has been valued in Chinese medicine for hundreds of years as a cure for a wide variety of ailments. Some more recently isolated compounds, mainly from sea cucumbers and starfish, and including those with antitumour, antiviral, anticoagulant and antimicrobial activity, are summarised below. When wild stocks decline, the demand created in the market place raises the price of the product and, consequently, culturing is more likely to become viable economically. As this review shows, there have been dramatic advances in the culture methods of sea urchins and sea cucumbers in the last 10-15 years, to the extent that one can conclude that currently the major obstacles to successful cultivation are indeed economic rather than biological. Hence the future of the echinoculture industry is closely linked to that of the fisheries, whose fate will ultimately determine the market forces that will shape this growing industry.

Mapping seabed habitats in the Firth of Lorn off the west coast of Scotland: evaluation and comparison of habitat maps produced using the acoustic ground-discrimination system, RoxAnn, and sidescan sonar

Abstract: In September 2003 a national workshop took place in the United Kingdom with the aim of assessing the accuracy of the acoustic ground-discrimination system (AGDS), RoxAnn ,a s a tool for mapping seabed habitats in Special Areas of Conservation (SACs). A heterogeneous area of seabed, approximately 1 km 2 in size, was selected for the study. The area was first surveyed using a sidescan-sonar system and a mosaic of the output was produced covering 100% of the survey area. Interpretation of the mosaic identified three acoustically distinct seabed types, the spatial distributions of which were mapped. Four RoxAnn data sets were then collected over the same area of seabed applying different survey parameters (e.g. different survey grids, track spacing, survey vessels, survey speeds, and RoxAnn systems). Extensive ground-truthing was carried out involving 26 drop-down video stations, and from these data six benthic classes (seabed habitats) were identified. Following interpolation of the RoxAnn track-point data to produce full spatial-coverage data, these sixseabed habitat categories were used to conduct supervised classification of the RoxAnn data to produce full-coverage habitat maps of the area for each of the four RoxAnn data sets. Comparisons were then made between the four RoxAnn maps and the sidescan-sonar interpreted map. The accuracy of each map was assessed and the application of this mapping approach for mapping seabed habitats in SACs is discussed.

Reef-aggregating behaviour by symbiotic eunicid polychaetes from cold-water corals : do worms assemble reefs?

Abstract: In the north-east Atlantic, the dominant reef-framework forming coral species, Lophelia pertusa and Madrepora oculata, form a symbiotic association with the polychaete worm Eunice norvegica. The polychaete -coral symbiosis was studied by visually observing and photographing live animals in aquaria over many months and using time-lapse video under infra-red lighting to record activity patterns. The polychaetes act as reef aggregating agents by joining coral colonies and enhancing the development of reef patches in deep water. The symbiosis was investigated using samples collected from a relatively shallow site in a Norwegian fjord and from a deeper open ocean site in the Porcupine Seabight. The potential functional roles of this symbiosis are considered. The reef aggregating behaviour of the polychaete symbiont allied with the ability of the coral host to anastomose its branches, the polychaete tubes and debris falling onto the reef structure will help to shift the balance between reef growth and (bio)erosion in favour of growth.

Evolution and distribution of TKE production and dissipation within stratified flow over topography

Abstract: [1] Analysis is presented of detailed observations of turbulence in stratified flow over topography. Key results are: 1) Directly measured dissipative energy losses due to skin friction and an internal hydraulic transition are relatively small, each accounting for approximately 10% of the mean flow energy loss, and 2) Production and dissipation of turbulent kinetic energy in flow over the topographic feature are in approximate balance throughout the transition from sub- to super-critical mean flow. The study is two-dimensional and a closed energy budget through direct measurement remains elusive; it is concluded that horizontal aspects of barotropic form drag such as eddy-shedding are responsible for as much as 30–40% of the mean flow energy loss.

Age-related trends in otolith chemistry of Merluccius merluccius from the north-eastern Atlantic Ocean and the western Mediterranean Sea

Abstract: Sagittal otoliths of European hake obtained from five geographic locations in the north-eastern Atlantic and western Mediterranean were examined using laser ablation and inductively coupled plasma mass spectrometry. Otolith sections were analysed for the isotopes 24 Mg, 55 Mn, 66 Zn, 85 Rb, 86 Sr, 138 Ba and 208 Pb, measured relative to 43 Ca counts. These analyses considered only age 0 (core area) and ages 1 to 3. Age-related trends in otolith elemental composition were observed in hake from all areas, but were masked by variability between locations. Elemental concentrations generally decreased outside the core, with some increase at age 3. The composition of the otolith core was very distinct from that of the other growth increments. In the Mediterranean, part of this differentiation was a result of Mn, which was present in the core at high concentrations compared with the rest of the otolith. Mediterranean otoliths also had higher concentrations of Sr, Zn and Ba in the core. For most samples a similar trend was observed, although samples from one of the Mediterranean areas showed some differences, mainly in the concentrations of Mg and Sr. These results provide new empirical evidence of the variation in elemental concentrations across hake otoliths with age, at least throughout the first 3 years of life.

Mixing and advection of a cold water cascade over the Wyville Thomson Ridge

Abstract: The Wyville Thomson Ridge forms part of the barrier to the meridional circulation across which cold Nordic Sea and Arctic water must traverse to reach the Atlantic Ocean. Overflow rates across the ridge are variable (but can be dramatic at times), and may provide a subtle indicator of significant change in the circulation in response to climate change. In spring 2003, a series of CTD sections were conducted during a large overflow event in which Norwegian Sea Deep Water (NSDW) cascaded down the southern side of the ridge into the Rockall Trough at a rate of between 1 and 2 Sv. The NSDW was partially mixed with overlying North Atlantic Water (NAW), and comprised about 1/3rd of the cascading water. The components of NAW and NSDW in the overflow were sufficiently large that there must have been a significant divergence of the inflow through the Faroe-Shetland Channel, and of the outflow through the Faroe Bank Channel. As the plume descended, its temperature near the sea bed warmed by over 3 °C in about a day. Although the slope was quite steep (0.03), the mean speed of the current (typically 0.36 m s −1 ) was too slow for significant entrainment of NAW to occur (the bulk Richardson number was of order 5). However, very large overturns (up to 50 m) were evident in some CTD profiles, and it is demonstrated from Thorpe scale estimates that the warming of the bottom waters was due to mixing within the plume. It is likely that some of the NSDW had mixed with NAW before it crossed the ridge. The overflow was trapped in a gully, which caused it to descend to great depth (1700 m) at a faster rate, and with less modification due to entrainment, than other overflows in the North Atlantic. The water that flowed into the northern part of the Rockall Trough had a temperature profile that ranged from about 3 to 8 °C. Water with a temperature of >6 °C probably escaped into the Iceland Basin, between the banks that line the north-western part of the Trough. Colder water (

Evidence of a latent oxidative burst in relation to wound repair in the giant unicellular chlorophyte dasycladus vermicularis1

Abstract: We investigated the kinetics and composition of the second phase of the wound repair process of Dasycladus vermicularis ([Scropoli] Krasser) using fluorescent probes, chromatography, UV spectroscopy, and histochemistry. Our new evidence supports the hypothesis that the second phase of wound repair (initiated at approximately 35-45 min post-injury) is based on the activation of an oxidative burst that produces micromolar H 2 O 2 levels. These results provide evidence of peroxidase activity at the wound site, real-time measurements of an oxidative burst, and catechol localization in wound plugs. Strong evidence is presented indicating that the biochemical machinery exists for oxidative cross-linking to ensue in the wound-healing process of D. vermicularis.

Polyandry in the ectoparasitic copepod Lepeophtheirus salmonis despite complex precopulatory and postcopulatory mate-guarding

Abstract: Lepeophtheirus salmonis (Kroyer) is an economically important pest on cultured salmonids in the North Atlantic, and has been implicated in declines of some wild salmonid popula- tions. Males inseminate newly-moulted adult females following the cementing of a pair of sper- matophores to the female's genital complex. Females can produce multiple pairs of eggstrings over a period of months, but this species is reported to be monogamous as a result of blockage of the female's copulatory ducts by the spermatophore tubules. On wild and farmed Atlantic salmon Salmo salar L., respectively, 88 and 78% adult females sea lice bore the typical pair of spermatophores, while 11 and 19% lacked spermatophores. A very few individuals (1% wild, 3% farmed) bore 3 or 4 spermatophores, showing that apparently successful multiple mating is possible. Multiple paternity was confirmed by dual-locus microsatellite typing of offspring for 3 of 7 females carrying 4 sper- matophores, but also for 2 of 3 females carrying a single pair of spermatophores. Probably most females on wild fish lose their initial spermatophores and are polygamous during their extended ovigerous lifetime, although effective blockage of the copulatory ducts by the first male almost cer- tainly assures single paternity of the first few pairs of eggstrings. The total level of polyandry or sperm competition faced by males may be relatively low. The ecological implications of multiple paternity are discussed within the context of integrated pest management as a strategy of ameliorating L. salmonis infestations impacting both wild and farmed salmonids.

Cryopreservation and conservation of microalgae: the development of a Pan-European scientific and biotechnological resource (the COBRA project).

Abstract: Microalgae are one of the most biologically important elements of worldwide ecology and could be the source of diverse new products and medicines COBRA (The Conservation of a vital european scientific and Biotechnological Resource: microAlgae and cyanobacteria) is the acronym for a European Union, RTD Infrastructures project (Contract No QLRI-CT-2001-01645) This project is in the process of developing a European Biological Resource Centre based on existing algal culture collections The COBRA project's central aim is to apply cryopreservation methodologies to microalgae and cyanobacteria, organisms that, to date, have proved difficult to conserve using cryogenic methods In addition, molecular and biochemical stability tests have been developed to ensure that the equivalent strains of microorganisms supplied by the culture collections give high quality and consistent performance Fundamental and applied knowledge of stress physiology form an essential component of the project and this is being employed to assist the optimisation of methods for preserving a wide range of algal diversity COBRA's "Resource Centre" utilises Information Technologies (IT) and Knowledge Management practices to assist project coordination, management and information dissemination and facilitate the generation of new knowledge pertaining to algal conservation This review of the COBRA project will give a summary of current methodologies for cryopreservation of microalgae and procedures adopted within the COBRA project to enhance preservation techniques for this diverse group of organisms

Spatially complex distribution of dissolved manganese in a fjord as revealed by high-resolution in situ sensing using the autonomous underwater vehicle Autosub.

Abstract: Loch Etive is a fjordic system on the west coast of Scotland. The deep waters of the upper basin are periodically isolated, and during these periods oxygen is lost through benthic respiration and concentrations of dissolved manganese increase. In April 2000 the autonomous underwater vehicle (AUV) Autosub was fitted with an in situ dissolved manganese analyzer and was used to study the spatial variability of this element together with oxygen, salinity, and temperature throughout the basin. Six along-loch transects were completed at either constant height above the seafloor or at constant depth below the surface. The ca. 4000 in situ 10-s-average dissolved Mn (Mnd) data points obtained provide a new quasi-synoptic and highly detailed view of the distribution of manganese in this fjordic environment not possible using conventional (water bottle) sampling. There is substantial variability in concentrations ( 600 nM) and distributions of Mnd. Surface waters are characteristically low in Mnd reflecting mixing of riverine and marine end-member waters, both of which are low in Mnd. The deeper waters are enriched in Mnd, and as the water column always contains some oxygen, this must reflect primarily benthic inputs of reduced dissolved Mn. However, this enrichment of Mnd is spatially very variable, presumably as a result of variability in release of Mn coupled with mixing of water in the loch and removal processes. This work demonstrates how AUVs coupled with chemical sensors can reveal substantial small-scale variability of distributions of chemical species in coastal environments that would not be resolved by conventional sampling approaches. Such information is essential if we are to improve our understanding of the nature and significance of the underlying processes leading to this variability.

A preliminary investigation of basin water response to climate forcing in a Scottish fjord: evaluating the influence of the NAO

Abstract: The sea lochs (fjords) of NW Scotland bridge the land–ocean interface in a region of Europe which is particularly well situated to monitor changes in westerly air streams Inter-annual atmospheric circulation changes at this latitude are largely governed by the North Atlantic Oscillation (NAO) Comparing two recent extreme NAO years, a two-dimensional model of Loch Sunart, NW Scotland, is used to examine the potential effects of climate oscillations on the magnitude and frequency of deep-water renewal events and the resulting water properties in the fjord basins In the upper basin of the fjord, meteorological forcing during the high NAO index year (1988–89) resulted in less-frequent deep-water renewal, greater variability in basin salinity and a lower annual-mean salinity (by 052) than that predicted for the low NAO index year (1995–96) In the main basin, variations in meteorological forcing had much less effect on basin water properties In both basins, predicted deep water inflow was significantly greater during the negative phase NAO, with annual inflow to the upper and main basins being respectively 50% and 300% greater during 1995–96 relative to 1988–99 Through a sensitivity analysis, the NAO is shown to affect upper basin water properties through the influence of low-frequency anomalies in the meteorological forcing, particularly the enhanced westerly wind stress associated with positive phases, which inhibits deep water renewal over the winter months The salinity of the main and upper basins respond differently to the boundary forcing due to differential tidal mixing above the respectively sub- and super-critical entrance sills Predictions of basin water isotope ratios are made by applying the salinity: δ 18 O water mixing line for the region to the salinity results; the combination of the weak dependence of δ 18 O water on salinity and the stable salinity predicted for the main basin suggests that some fjord basins may provide ideal sites for palaeotemperature studies

Ecology of bathyal polychaete fauna at an Arctic-Atlantic boundary (Faroe-Shetland Channel, North-east Atlantic)

Abstract: By reference to a series of 15 sampling stations spanning the West Shetland Slope (150–1000 m; Faroe–Shetland Channel, North-east Atlantic) we examined the potential environmental controls on the standing stock, diversity and composition of the polychaete fauna. In contrast to the majority of studied bathyal environments, the Faroe–Shetland Channel has a highly complex and dynamic hydrographic regime, particularly notable for extreme thermal variability at mid-slope depths (i.e. 7°C range at ca. 500 m). Contrary to general expectation, polychaete biomass increased (rather than decreased) with depth. Species diversity exhibited a parabolic pattern with depth, maximum diversity occurring at depths of 350–550 m, rather shallower than observed in other bathyal studies, and possibly linked with a maximum in habitat temperature range. Multivariate analyses of faunal composition suggested a separation of the sampling stations into a shallower and a deeper group, with temperature exerting a major control on polyc...

Bromine is an endogenous component of a vanadium bromoperoxidase.

Abstract: On the basis of EXAFS and MS/MS experimental results and a reinterpretation of the electron density map obtained by X-ray crystallography, we describe a new post-translational modification, that is, a 3,5-dibromotyrosine residue that is incorporated in the polypeptide chain of a vanadium haloperoxidase.

'Biofiltration and biofouling on artificial structures in Europe: the potential for mitigating organic impacts

Abstract: Man-made structures deployed either deliberately or accidentally into the sea are subject to levels of biofouling The resultant communities are usually dominated by sessile marine invertebrates that tend to utilize suspension-feeding for nutritional gain from the water column Not all suspension-feeders are filtration-feeders but in general terms a large suspension-feeding community will provide varying scales of water filtration The potential for utilizing some form of in-water biofiltration in association with localised organic enrichment has long been suggested but with few quantitative estimates of probable efficacy The major taxa that are likely to be relevant to the process of biofiltration are discussed in relation to the functional classification of suspension-feeders In order to generate estimates of biofiltration potential, activity rates of the major functional and taxonomic groups of suspension-feeders are derived through a review of the general mode of suspension-feeding, the predominant food sources with the size range of particles retained and individual suspension-feeding rates that are scaled up to the population level However, any naturally occurring fouling community will consist of a number of species and so estimates of multispecies suspension-feeding, interspecific interactions, rates of biodeposition and nutrient release are derived The rates and densities of biofouling are dependent of the abiotic and biotic characteristics of the receiving environment and the types of materials used in the provided substrate The factors affecting biofouling are discussed in relation to existing examples of artificial structures found in European waters or waters of relevance to Europe In-water outputs from finfish mariculture provide examples of localised point sources of organic enrichment that could benefit from the associated deployments of biological filters Using estimates of filtration clearance rates combined with the major taxa thought most likely to dominate any filtering community, probable scales of biofiltration required in order to influence the levels of mariculture discharges are calculated Although, in theory, biofilters in open system finfish mariculture may reduce the levels of organic impact, the scale of intervention required to make a significant effect would probably exceed any form of economic viability