Showing papers by "Shandong Normal University published in 2014"
TL;DR: This review describes the extant knowledge on the teleostean mucosal adaptive immune mechanisms, which is relevant for the development of oral or mucosal vaccines, and suggests the advancement of fish vaccines.
237 citations
TL;DR: A novel nanoprobe for detection and imaging of ascorbic acid in living cells and in vivo based on the specific reaction of cobalt oxyhydroxide and AA is reported, which shows high selectivity and an instantaneous response.
Abstract: The development of a specific reaction of nanomaterials and reactive species is of fundamental importance for the determination of biomolecules. Here we report a novel nanoprobe for detection and imaging of ascorbic acid (AA) in living cells and in vivo based on the specific reaction of cobalt oxyhydroxide (CoOOH) and AA. Persistent luminescence nanoparticles (PLNPs) were used as the luminescence unit, and CoOOH nanoflakes served as the quencher. When CoOOH was modified on the surface of the PLNPs, the luminescence of the PLNPs was efficiently quenched by the CoOOH. In the presence of AA, CoOOH was reduced to Co(2+) and the luminescence of PLNPs was restored. The nanoprobe showed high selectivity and an instantaneous response. The luminescence property permits detection and imaging without external excitation, which could effectively avoid background noise and scattering of light from biological matrixes produced by in situ excitation. The current strategy provides an effective platform for monitoring and imaging reactive species in living cells and in vivo.
190 citations
TL;DR: A porous Cu(I)-MOF was constructed from CuI and 1-benzimidazolyl-3,5-bis(4-pyridyl)benzene to be a highly sensitive naked-eye colorimetric sensor to successively detect water and formaldehyde species in a single-crystal-to-single-Crystal fashion.
185 citations
TL;DR: This review of recent advances in using functional nanomaterials for imaging, primarily cellular imaging, is summarized.
Abstract: Nanomaterials with unique optical properties have shown great promise as probes for cellular imaging Based on these properties, a wide range of plasmonic, fluorescent and Raman probes have been designed and prepared Nanomaterials of different sizes and shapes have also been functionalized with various types of biomolecules, such as antibodies, DNA or RNA, which are actively exploited to realize targeted imaging In this review, we will summarize recent advances in using functional nanomaterials for imaging, primarily cellular imaging These nanomaterials are categorized based on their conducting properties, ie conductors, semiconductors and insulators
179 citations
TL;DR: The metal-organic framework can be regarded as a novel molecular sieve-like material with a chiral separation function based on the relative sizes of the chiral channels and the resolved molecules.
Abstract: Homochiral metal–organic frameworks with fine-tuned pore sizes/walls and large surface areas are promising porous materials for enantioseparation considering the traditional zeolite molecular sieves have no chirality. Using enantiopure pyridyl-functionalized salen [(N-(4-Pyridylmethyl)-l-leucine·HBr)] as a starting material, we have prepared a noninterpenetrated three-dimensional homochiral metal organic framework {[ZnLBr]·H2O}n, which was further used as a chiral stationary phase for high-performance liquid chromatography to enantioseparate racemic drugs, showing excellent performances in enantioseparation of drugs. The metal–organic framework can be regarded as a novel molecular sieve-like material with a chiral separation function based on the relative sizes of the chiral channels and the resolved molecules.
159 citations
TL;DR: A simple, rapid, isothermal, and ultrasensitive homogeneous electrochemical DNA biosensing platform for target DNA and protein detection has been developed on the basis of an exonuclease III (Exo III)-aided autocatalytic target recycling strategy.
Abstract: Homogenous electrochemical biosensor has attracted substantial attention owing to its simplicity, rapid response, and improved recognition efficiency compared with heterogeneous biosensor, but the relatively low detection sensitivity and the limited detection analytes prohibit its potential applications To address these issues, herein, a simple, rapid, isothermal, and ultrasensitive homogeneous electrochemical DNA biosensing platform for target DNA and protein detection has been developed on the basis of an exonuclease III (Exo III)-aided autocatalytic target recycling strategy A ferrocene-labeled hairpin probe (HP1) is ingeniously designed, which contains a protruding DNA fragment at 3′-termini as the recognition unit for target DNA Also, the DNA fragment that could be used as secondary target analogue was introduced, but it was caged in the stem region of HP1 In the presence of target DNA, its recognition with the protruding fragment of HP1 triggered the Exo III cleavage process, accompanied with th
149 citations
TL;DR: A novel fluorescence nanosensor based on a molecularly imprinted spatial structure and boronate affinity that is well-suited for monitoring glycoproteins selectively is described that was successfully applied to the detection of HRP in biological fluids.
Abstract: Glycoproteins are closely associated with the occurrence of diverse diseases, and they have been used as biomarkers and therapeutic targets in clinical diagnostics. Currently, mass spectrometry has proven to be a powerful tool for glycoprotein analysis, but it is almost impossible to directly identify glycoproteins without the preparation and pretreatment of samples. Furthermore, biological samples, especially proteins, are damaged by this process. Herein, we describe a novel fluorescence nanosensor based on a molecularly imprinted spatial structure and boronate affinity that is well-suited for monitoring glycoproteins selectively. Results showed that the recognition performance of the nanosensor for glycoproteins was regulated by controlling the pH value and temperature. Moreover, the nanosensor was successfully applied to the detection of HRP in biological fluids. This study provides a facile and efficient fluorescence tool for glycoprotein detection in clinical diagnostics.
141 citations
TL;DR: This biocompatible nano-MOF probe exhibits rapid response, excellent selectivity, and hypotoxicity in in situ detection of H2S and represents the most sensitive fluorescence probe for selective H 2S detection under physiological pH.
Abstract: Hydrogen sulfide (H2S) has been regarded as the third important gaseous signaling molecule involved in human physiological and pathological processes. Due to the high reactive and diffusible properties of H2S, real-time detection of H2S fluctuations in living biological specimens is crucial. Here, we present a Cu(II)-metalated 3D porous nanoscale metal–organic framework (nano-MOF) {CuL[AlOH]2}n (PAC; H6L = meso-tetrakis(4-carboxylphenyl)porphyrin) and successfully employ this nano-MOF as a novel heterogeneous fluorescence probe for H2S detection. As far as we know, nano-MOFs have never been used as selective fluorescence probes for H2S detection. On the basis of the advantages of nano-MOF materials, this biocompatible nano-MOF probe exhibits rapid response, excellent selectivity, and hypotoxicity in in situ detection of H2S and represents the most sensitive fluorescence probe for selective H2S detection under physiological pH. In addition, confocal imaging was achieved successfully in living cells.
138 citations
TL;DR: In this paper, the problem of global exponential stability for impulsive cellular neural networks with time-varying delays and supremums was studied and new stability criteria were proved using Young's inequality and Lyapunov-like functions.
Abstract: SUMMARY
In this paper, we study the problem of global exponential stability for impulsive cellular neural networks with time-varying delays and supremums. Using Young's inequality and Lyapunov-like functions, new stability criteria are proved. Because supremums and impulses are relevant in various contexts, including problems in the theory of automatic control, our results can be applied in the qualitative investigations of many practical problems of diverse interest. Copyright © 2013 John Wiley & Sons, Ltd.
121 citations
TL;DR: It is found that salt acclimated Arabidopsis young seedlings can survive subsequent 200 mM NaCl stress and MAPK6, a major component of the ethylene signaling pathway, was found to play a crucial role in SA.
Abstract: Plants can successfully improve their resistance to previously lethal salinity stress by a short exposure to low levels of salt stress, a process known as salt acclimation (SA). In spite of its fundamental significance in theoretical study and agricultural practice, the molecular mechanisms underlying plant SA remain elusive. In this study, we found that salt acclimated Arabidopsis young seedlings can survive subsequent 200 mM NaCl stress. RNA-seq was performed to analyze the genome-wide transcriptional response under SA conditions. Among 518 differentially expressed genes (DEGs) under SA, 366 up-regulated genes were enriched for cell wall biosynthesis, osmoregulation, oxidative stress, or transcription factors. Seven DEGs participate in the synthesis of lignin and 24 DEGs encode plant cell wall proteins, suggesting the importance of cell wall remodeling under SA. Furthermore, in comparison to non-acclimated salt stress, 228 of 245 DEGs were repressed by acclimated salt stress, including many genes related to ethylene biosynthesis and signaling pathway. In addition, MAPK6, a major component of the ethylene signaling pathway, was found to play a crucial role in SA. Our transcriptomic analysis has provided important insight on the roles of transcription factors, cell wall remodeling, and the ethylene biosynthesis and signaling pathways during SA in Arabidopsis.
120 citations
TL;DR: The study examined the prevalence, frequency, and coexistence of psychological aggression (PA), corporal punishment (CP), and severe physical abuse (SPA) in mainland China and highlighted the importance of studying these three types of parental harsh discipline simultaneously and intervening in harsh discipline by mothers and fathers within the same family.
TL;DR: Experimental results show that, RWO-Sampling statistically does much better than alternative methods on imbalanced data sets when implementing common baseline algorithms.
TL;DR: The results demonstrated that the La-AA was a promising adsorbent for effective removal of F(-) from water.
TL;DR: The synthesis and screening of Cy-3-NO2 showed simultaneous fluorescence sensing ability towards glutathione and cysteine under single excitation, and its application in living cell imaging is reported.
TL;DR: Considering the counterattack mechanism of the rumor spreading, two new models are introduced: Susceptible–Infective–Counterattack–Refractory (S ICR) model and adjusted-SICR model and the self-resistance parameter τ is introduced, and the influence of this parameter on rumor spreading is studied.
Abstract: Rumor is an important form of social interaction. However, spreading of harmful rumors could have a significant negative impact on the well-being of the society. In this paper, considering the counterattack mechanism of the rumor spreading, we introduce two new models: Susceptible–Infective–Counterattack–Refractory (SICR) model and adjusted-SICR model. We then derive mean-field equations to describe their dynamics in homogeneous networks and conduct the steady-state analysis. We also introduce the self-resistance parameter τ , and study the influence of this parameter on rumor spreading. Numerical simulations are performed to compare the SICR model with the SIR model and the adjusted-SICR model, respectively, and we investigate the spreading peak of the rumor and the final size of the rumor with various parameters. Simulation results are congruent exactly with the theoretical analysis. The experiment reveals some interesting patterns of rumor spreading involved with counterattack force.
TL;DR: A new small molecule fluorescent probe, possessing near-infrared (NIR) emission and an unusually large Stokes shift, is demonstrated, which can be readily taken up by live cells and mitochondria, and track subtle pH changes with effectively reduced biological background fluorescence and improved measurement accuracy.
TL;DR: The results suggest that Thellungiella under salt stress displays high resistance to photoinhibition and that increased concentrations of unsaturated fatty acids in membrane lipids enhances the tolerance of photosystem II to salt stress.
Abstract: The effect of salinity on plant growth, chlorophyll content, photosynthetic parameters, photochemical efficiency of PSII, membrane lipid content and fatty-acid composition of halophyte Thellungiella halophila and glycophyte Arabidopsis thaliana was investigated to examine the possible role of unsaturated fatty acids in photosynthesis under saline conditions. The growth of Arabidopsis was significantly decreased by the 100 and 200 mM NaCl treatments; however, there was no significant difference in the fresh and dry weight of Thellungiella at different concentrations of NaCl. Exposure of Arabidopsis to salt resulted in a progressive decline in chlorophyll content, while there was no significant change in that of Thellungiella. The net photosynthetic rate, maximal photochemical efficiency of PSII (F
v/F
m) and actual PSII efficiency were significantly reduced in Arabidopsis but remained unaffected in Thellungiella. Arabidopsis under NaCl treatment showed decreased PG levels and decreased values for the unsaturated fatty acid content and the double bond index (DBI) of monogalactosyldiacylglycerols and phosphatidylglycerols; these values significantly increased in Thellungiella under NaCl treatment, as did the DBI values of digalactosyldiacylglycerols and sulphoquinovosyldiacylglycerols. These results suggest that Thellungiella under salt stress displays high resistance to photoinhibition and that increased concentrations of unsaturated fatty acids in membrane lipids enhances the tolerance of photosystem II to salt stress.
TL;DR: A novel nanoprobe, based on the manganese dioxide-modified PLNPs, that can detect and image glutathione in living cells and in vivo and can allow detection and imaging without external excitation and avoid the background noise originating from the in situ excitation is demonstrated.
Abstract: Persistent luminescence nanoparticles (PLNPs) hold great promise for the detection and imaging of biomolecules. Herein, we have demonstrated a novel nanoprobe, based on the manganese dioxide (MnO2 )-modified PLNPs, that can detect and image glutathione in living cells and in vivo. The persistent luminescence of the PLNPs can be efficiently quenched by the MnO2 nanosheets. In the presence of glutathione (GSH), MnO2 was reduced to Mn(2+) and the luminescence of PLNPs can be restored. The persistent luminescence property can allow detection and imaging without external excitation and avoid the background noise originating from the in situ excitation. This strategy can offer a promising platform for detection and imaging of reactive species in living cells or in vivo.
TL;DR: The results suggest that overexpression of AtZFP1 enhanced salt tolerance by maintaining ionic balance and limiting oxidative and osmotic stress.
Abstract: The CCCH type zinc finger proteins are a super family involved in many aspects of plant growth and development. In this study, we investigated the response of one CCCH type zinc finger protein AtZFP1 (At2g25900) to salt stress in Arabidopsis. The expression of AtZFP1 was upregulated by salt stress. Compared to transgenic strains, the germination rate, emerging rate of cotyledons and root length of wild plants were significantly lower under NaCl treatments, while the inhibitory effect was significantly severe in T-DNA insertion mutant strains. At germination stage, it was mainly osmotic stress when treated with NaCl. Relative to wild plants, overexpression strains maintained a higher K+, K+/Na+, chlorophyll and proline content, and lower Na+ and MDA content. Quantitative real-time PCR analysis revealed that the expression of stress related marker genes KIN1, RD29B and RD22 increased more significantly in transgenic strains by salt stress. Overexpression of AtZFP1 also enhanced oxidative and osmotic stress tolerance which was determined by measuring the expression of a set of antioxidant genes, osmotic stress genes and ion transport protein genes such as SOS1, AtP5CS1 and AtGSTU5. Overall, our results suggest that overexpression of AtZFP1 enhanced salt tolerance by maintaining ionic balance and limiting oxidative and osmotic stress.
TL;DR: A highly sensitive and homogeneous detection of membrane protein on single living cells by aptamer and nicking enzyme assisted fluorescence signal amplification in microfluidic droplets is reported.
Abstract: Membrane proteins play vital roles in numerous physiological functions. Recently, they have been considered as candidate biomarkers for cancer and recognized as major drug targets. So, accurate, sensitive, and high-throughput quantitative detection of the membrane proteins is crucial for better understanding their roles in cancer cells and further validating their function in clinical research. Here, we report a highly sensitive and homogeneous detection of membrane protein on single living cells by aptamer and nicking enzyme assisted fluorescence signal amplification in microfluidic droplets. The homogeneous reaction based on the membrane protein-triggered conformation alteration of hairpin probe can improve the detection accuracy with elimination of several washing and separation steps. The microfluidic system provides a high-throughput platform for the detection of a single cell, and the highly monodisperse droplet can function as an independent microreactor for the aptamer and nicking enzyme assisted fluorescence signal amplification, coordinating with the small volume of the confined space (a droplet), increased reaction rate, and highly sensitive detection of membrane protein on single cell can be reached.
TL;DR: A ratiometric electrochemiluminescent biosensor for the detection of microRNAs based on cyclic enzyme amplification and distance dependent resonance energy transfer was reported for the first time.
TL;DR: A stable, sensitive, and specific miRNAs detection method on the basis of cooperative amplification combining with the graphene oxide (GO) fluorescence switch-based circular exponential amplification and the multimolecules labeling of SYBR Green I (SG) to offer a new approach for reliable quantification of miRNA in medical research and early clinical diagnostics.
Abstract: MicroRNAs (miRNAs) play significant roles in a diverse range of biological progress and have been regarded as biomarkers and therapeutic targets in cancer treatment. Sensitive and accurate detection of miRNAs is crucial for better understanding their roles in cancer cells and further validating their function in clinical diagnosis. Here, we developed a stable, sensitive, and specific miRNAs detection method on the basis of cooperative amplification combining with the graphene oxide (GO) fluorescence switch-based circular exponential amplification and the multimolecules labeling of SYBR Green I (SG). First, the target miRNA is adsorbed on the surface of GO, which can protect the miRNA from enzyme digest. Next, the miRNA hybridizes with a partial hairpin probe and then acts as a primer to initiate a strand displacement reaction to form a complete duplex. Finally, under the action of nicking enzyme, universal DNA fragments are released and used as triggers to initiate next reaction cycle, constituting a new circular exponential amplification. In the proposed strategy, a small amount of target miRNA can be converted to a large number of stable DNA triggers, leading to a remarkable amplification for the target. Moreover, compared with labeling with a 1:1 stoichiometric ratio, multimolecules binding of intercalating dye SG to double-stranded DNA (dsDNA) can induce significant enhancement of fluorescence signal and further improve the detection sensitivity. The extraordinary fluorescence quenching of GO used here guarantees the high signal-to-noise ratio. Due to the protection for target miRNA by GO, the cooperative amplification, and low fluorescence background, sensitive and accurate detection of miRNAs has been achieved. The strategy proposed here will offer a new approach for reliable quantification of miRNAs in medical research and early clinical diagnostics.
TL;DR: Using the impulsive delay differential inequality technique, several sufficient conditions ensuring the exponential synchronization of chaotic delayed networks are derived, which can be easily checked by LMI Control Toolbox in Matlab.
TL;DR: In this article, the effects of NaCl stress on the growth and photosynthetic characters of Ulmus pumila L. seedlings were investigated under sand culture condition, and it was shown that the accumulation of Na+ in already expanded leaves might reduce damage to the expanding leaves and help U. pumilla endure high salinity.
Abstract: The effects of NaCl stress on the growth and photosynthetic characters of Ulmus pumila L. seedlings were investigated under sand culture condition. With increasing NaCl concentration, main stem height, branch number, leaf number, and leaf area declined, while Na+ content and the Na+/K+ ratio in both expanded and expanding leaves increased. Na+ content was significantly higher in expanded leaves than in those just expanding. Chlorophyll (Chl) a and Chl b contents declined as NaCl concentration increased. The net photosynthetic rate, intercellular CO2 concentration, stomatal conductance, and transpiration rate also declined, but stomatal limitation value increased as NaCl concentration increased. Both the maximal quantum yield of PSII photochemistry and the effective quantum yield of PSII photochemistry declined as NaCl concentration rose. These results suggest that the accumulation of Na+ in already expanded leaves might reduce damage to the expanding leaves and help U. pumila endure high salinity. The reduced photosynthesis in response to salt stress was mainly caused by stomatal limitation.
TL;DR: Theoretical analysis and experimental demonstration prove that the system in combination with a double-pass computer-generated hologram and a black-and-white pattern can generate vector beams with arbitrary polarization and complex amplitude by respectively controlling the complex amplitudes of two orthogonal polarization components of the beams.
Abstract: A flexible approach is presented to generate vector beams with arbitrary polarization and complex amplitude by means of two cascaded transmissive liquid crystal spatial light modulators (LCSLMs). The configuration of the cascaded LCSLM system and its modulation characteristic are introduced. Theoretical analysis and experimental demonstration prove that the system in combination with a double-pass computer-generated hologram and a black-and-white pattern can generate vector beams with arbitrary polarization and complex amplitude by respectively controlling the complex amplitudes of two orthogonal polarization components of the beams. Using this system, we successfully generate radially polarized vector beams with helical phase distributions and vector Bessel beams with inhomogeneous amplitude distributions in experiments.
TL;DR: Both molecular and physiological studies provide strong in vivo evidence that SsHKT1;1 mainly acts as a potassium transporter in heterologous expression systems and S. salsa is involved in salt tolerance by taking part in the maintenance of cytosolic cation homeostasis, particularly, in the Maintenance of K+ nutrition under salinity.
Abstract: SsHKT1;1, a HKT1 homologue, was isolated from the C3 halophyte Suaeda salsa L. and its ion transport properties were investigated in heterologous systems. The expression of SsHKT1;1 suppressed a K+ transport-defective phenotype of the yeast strain CY162 (Δtrk1Δtrk2), suggesting the enhancement of K+ uptake with SsHKT1;1. However, it did not suppress the salt-sensitive phenotype of the yeast strain G19 (Δena1–4), which lacks a major component of Na+ efflux. Transgenic Arabidopsis thaliana (L.) Heynh. plants overexpressing SsHKT1;1 showed enhanced salt tolerance and increased shoot K+ concentration, whereas no significant changes in shoot Na+ concentration were observed. S. salsa was also used to investigate K+ uptake properties under salinity. The K+ transporters in the roots selectively mediated K+ uptake irrespective of external Na+ and their inhibitor did not affect Na+ uptake at low K+. Thus, both molecular and physiological studies provide strong in vivo evidence that SsHKT1;1 mainly acts as a potassium transporter in heterologous expression systems and S. salsa, and that it is involved in salt tolerance by taking part in the maintenance of cytosolic cation homeostasis, particularly, in the maintenance of K+ nutrition under salinity.
TL;DR: Three-dimensional-excitation emission matrix fluorescence showed that ZVI enhanced organic material diversity and Fe(2+) dissolution from ZVI helped reduce the start-up time of aerobic granulation and increased the extracellular polymeric substance content.
TL;DR: In this article, the authors investigated the mutual interactions between three differently substituted B-ring hydroxyl groups (Pelargonidin-3-O-glucoside, P3G, C3G and D3G) and human serum albumin (HSA) under physiological pH conditions.
TL;DR: It is found that DHA shows a dose-dependent inhibition of proliferation and migration of in HUVECs and down-regulates the mRNA and protein expression of VEGfr2 in endothelial cells, suggesting defective NF-κB signaling may underlie the observed effects of DHA on VEGFR2 expression.
Abstract: The anti-malarial agent dihydroartemisinin (DHA) has strong anti-angiogenic activity. This study aimed to investigate the molecular mechanism underlying this effect of DHA on angiogenesis. We found that DHA shows a dose-dependent inhibition of proliferation and migration of in HUVECs. DHA specifically down-regulates the mRNA and protein expression of VEGFR2 in endothelial cells. Treatment with DHA increases IκB-α protein and blocks nuclear translocation of NF-κB p65. In addition, DHA directly regulates VEGFR2 promoter activity through p65 binding motif, and decreases the binding activity of p65 and VEGFR2 promoter, suggesting defective NF-κB signaling may underlie the observed effects of DHA on VEGFR2 expression. In the presence of the NF-κB inhibitor PDTC, DHA could not further repress VEGFR2. Co-treatment with PDTC and DHA produced minimal changes compared to the effects of either drug alone in in vitro angiogenesis assays. Similar findings were found in vivo through a mouse retinal neovascularization model examining the effects of PDTC and DHA. Our data suggested that DHA inhibits angiogenesis largely through repression of the NF-κB pathway. DHA is well tolerated, and therefore may be an ideal candidate to use clinically as an angiogenesis inhibitor for cancer treatment.
TL;DR: It is revealed that the NLRP3 inflammasome exerts a critical role in host innate immune responses against M. canis infection, and the data suggest that diseases that result from M.Canis infection might be controlled by regulating the activation of inflammaomes.
Abstract: Microsporum canis is a pathogenic fungus with worldwide distribution that causes tinea capitis in animals and humans. M. canis also causes invasive infection in immunocompromised patients. To defy pathogenic fungal infection, the host innate immune system is the first line of defense. As an important arm of innate immunity, the inflammasomes are intracellular multiprotein complexes that control the activation of caspase-1, which cleaves proinflammatory cytokine pro-interleukin-1β (IL-1β) into its mature form. To determine whether the inflammasome is involved in the host defense against M. canis infection, we challenged human monocytic THP-1 cells and mouse dendritic cells with a clinical strain of M. canis isolated from patients with tinea capitis. We found that M. canis infection triggered rapid secretion of IL-1β from both THP-1 cells and mouse dendritic cells. Moreover, by using gene-specific shRNA and competitive inhibitors, we determined that M. canis-induced IL-1β secretion was dependent on NLRP3. The pathways proposed for NLRP3 inflammasome activation, namely, cathepsin B activity, K(+) efflux, and reactive oxygen species production, were all required for the inflammasome activation triggered by M. canis. Meanwhile, Syk, Dectin-1, and Card9 were found to be involved in M. canis-induced IL-1β secretion via regulation of pro-IL-1β transcription. More importantly, our data revealed that M. canis-induced production of IL-1β was dependent on the NLRP3 inflammasome in vivo. Together, this study unveils that the NLRP3 inflammasome exerts a critical role in host innate immune responses against M. canis infection, and our data suggest that diseases that result from M. canis infection might be controlled by regulating the activation of inflammasomes.