Shivaji University

About: Shivaji University is a education organization based out in Kolhāpur, Maharashtra, India. It is known for research contribution in the topics: Thin film & Scanning electron microscope. The organization has 3078 authors who have published 5295 publications receiving 115397 citations.

Biodegradation and detoxification of reactive textile dye by isolated Pseudomonas sp. SUK1.

Abstract: An isolated bacterium from a textile disposal site, Pseudomonas sp SUK1, has the ability to decolorize the reactive textile dyes and methyl orange This bacterium showed the potential to decolorize the textile dye Reactive Blue 59 at a high concentration (5 g/L(-1)), which is frequently used in the textile industry of Solapur, India Induction in the activities of lignin peroxidase, azoreductase, and dichlorophenol indophenol reductase was observed during the decolorization of Methyl Orange and Reactive Blue 59 Methyl Orange (as model azo dye) was used to understand the mechanism of biodegradation by Pseudomonas sp SUK1 The final product was identified as 1,4-benzenediamine, N, N-dimethyl by gas chromatography-mass spectroscopy Microbial and phytotoxicity studies revealed the nontoxic nature of the products of Reactive Blue 59

Mössbauer, Raman, and Magnetoresistance Study of Aluminum-Based Iron Oxide Thin Films

Abstract: Thin films of Al-based hematite iron oxide were synthesized by spray pyrolysis in aqueous medium onto the glass microslides. The compact and homogeneous distribution of grains (spindle-shaped hematite nanostructures) with varying sizes has been observed in surface morphological studies. The room temperature Mossbauer study has been carried out to monitor the local environment around Fe cations and valence state of Fe ions. Mossbauer and micro-Raman (low temperature) results suggest that oxygen vacancies cause cation redistribution between the interstitial sites resulting in magnetic ordering. The variation of magnetoresistance in low magnetic field (<3 kOe) is also reported.

Establishment of embryogenic cell suspension cultures and Agrobacterium-mediated transformation in an important Cavendish banana cv. Robusta (AAA)

Abstract: Establishment of an efficient protocol for regeneration and genetic transformation is required in banana for the incorporation of useful traits. Therefore an efficient method has been developed for somatic embryogenesis, plant regeneration and transformation of Cavendish banana cultivar Robusta (AAA). Embryogenic cell suspension culture (ECS) was established using immature male flowers. Percentage appearance of embryogenic callus and distinct globular embryos was 10.3 and 11.1, respectively. ECS obtained was cocultivated under different cocultivation conditions with Agrobacterium tumefaciens strain EHA105 harboring pCAMBIA 1301 plant expression vector. Up to 30 transgenic plants/50 mg settled cell volume (SCV) was obtained with cocultivation in semisolid medium whereas no transgenics could be obtained with parallel experiments carried out in liquid medium. Histochemical GUS assay in different tissues of putatively transformed plants demonstrated expression of uidA gene. Among the putatively transformed plants obtained, a set of 4 were confirmed by PCR analysis and stable integration of the transgene by Southern analysis. GUS specific activity measured by a MUG (4-methylumbelliferyl-β-d-glucuronide) based flourometric assay revealed increase in transient GUS expression in semisolid as well as liquid cocultivation with centrifugation. This is the first report showing somatic embryogenesis and Agrobacterium tumefaciens mediated transformation using embryogenic cell suspension cultures in an important Cavendish banana cultivar Robusta. The present protocol will make possible agronomic improvement of this important commercially grown cultivar by introduction of disease resistance characteristics and antisense-mediated delayed fruit ripening strategies. Further, it will also assist in functional characterization of new gene or promoter elements isolated from this or other cultivars of banana.