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Showing papers by "Wroclaw University of Environmental and Life Sciences published in 2020"


Journal ArticleDOI
TL;DR: A significantly decreased growth rate and increased doubling time of cases was observed, which is most likely due to Chinese lockdown measures, which seem to have a potential to slow down the spread of COVID-19.
Abstract: BACKGROUND With its epicenter in Wuhan, China, the COVID-19 outbreak was declared a Public Health Emergency of International Concern by the World Health Organization (WHO). Consequently, many countries have implemented flight restrictions to China. China itself has imposed a lockdown of the population of Wuhan as well as the entire Hubei province. However, whether these two enormous measures have led to significant changes in the spread of COVID-19 cases remains unclear. METHODS We analyzed the available data on the development of confirmed domestic and international COVID-19 cases before and after lockdown measures. We evaluated the correlation of domestic air traffic to the number of confirmed COVID-19 cases and determined the growth curves of COVID-19 cases within China before and after lockdown as well as after changes in COVID-19 diagnostic criteria. RESULTS Our findings indicate a significant increase in doubling time from 2 days (95% CI: 1.9-2.6) to 4 days (95% CI: 3.5-4.3), after imposing lockdown. A further increase is detected after changing diagnostic and testing methodology to 19.3 (95% CI: 15.1-26.3), respectively. Moreover, the correlation between domestic air traffic and COVID-19 spread became weaker following lockdown (before lockdown: r = 0.98, P < 0.05 vs after lockdown: r = 0.91, P = NS). CONCLUSIONS A significantly decreased growth rate and increased doubling time of cases was observed, which is most likely due to Chinese lockdown measures. A more stringent confinement of people in high risk areas seems to have a potential to slow down the spread of COVID-19.

982 citations


Journal ArticleDOI
TL;DR: The number of flight routes as well as total passenger volume are highly relevant risk factors for the spread of current COVID-19 and limited but not terminated air traffic can be a feasible option to prevent a long-term crisis.
Abstract: Background The World Health Organization (WHO) has declared the current outbreak of the novel coronavirus (COVID-19) a global pandemic. Many countries are facing increasing numbers of COVID-19 cases, which are, in their origin mostly attributed to regular international flight connections with China. This study aims to investigate this relation by analyzing available data on air traffic volume and the spread of COVID-19 cases. Methods and findings: We analyzed available data on current domestic and international passenger volume and flight routes and compared these to the distribution of domestic and international COVID-19 cases. Results Our data indicate a strong linear correlation between domestic COVID-19 cases and passenger volume for regions within China (r2 = 0.92, p = 0.19) and a significant correlation between international COVID-19 cases and passenger volume (r2 = 0.98, p Conclusions The number of flight routes as well as total passenger volume are highly relevant risk factors for the spread of current COVID-19. Multiple regions within Asia, as well as some in North America and Europe are at serious risk of constant exposure to COVID-19 from China and other highly infected countries. Risk for COVID-19 exposure remains relatively low in South America and Africa. If adequate measures are taken, including on-site disease detection and temporary passenger quarantine, limited but not terminated air traffic can be a feasible option to prevent a long-term crisis. Reasonable risk calculations and case evaluations per passenger volume are crucial aspects which must be considered when reducing international flights.

145 citations


Journal ArticleDOI
01 Jan 2020
Abstract: Nature-based solutions (NBS) can protect, manage and restore natural or modified ecosystems. They are a multidisciplinary, integrated approach to address societal challenges and some natural hazards effectively and adaptively, simultaneously providing human well-being and biodiversity benefits. NBS applications can be easily noticed in circular cities, establishing an urban system that is regenerative and accessible. This paper aims to offer a review on NBS for urban water management from the literature and some relevant projects running within the COST Action ‘Implementing nature-based solutions for creating a resourceful circular city’. The method used in the study is based on a detailed tracking of specific keywords in the literature using Google Scholar, ResearchGate, Academia.edu, ScienceDirect and Scopus. Based on this review, three main applications were identified: (i) flood and drought protection; (ii) the water-food-energy nexus; and (iii) water purification. The paper shows that NBS provide additional benefits, such as improving water quality, increasing biodiversity, obtaining social co-benefits, improving urban microclimate, and the reduction of energy consumption by improving indoor climate. The paper concludes that a systemic change to NBS should be given a higher priority and be preferred over conventional water infrastructure.

142 citations


Journal ArticleDOI
TL;DR: The data indicate that countries with lower HAQ-index may either underreport COVID-19 cases or are unable to adequately detect them, and the number of non-travel related COVID -19 cases seem to continuously increase in the HAZ-cohort of countries with higher medical standards.
Abstract: Background With its epicenter in Wuhan, China, the COVID-19 outbreak was declared a pandemic by the World Health Organization (WHO). While many countries have implemented flight restrictions to China, an increasing number of cases with or without travel background to China are confirmed daily. These developments support concerns on possible unidentified and unreported international COVID-19 cases, which could lead to new local disease epicenters. Methods We have analyzed all available data on the development of international COVID-19 cases from January 20th, 2020 until February 18th, 2020. COVID-19 cases with and without travel history to China were divided into cohorts according to the Healthcare Access and Quality Index (HAQ-Index) of each country. Chi-square and Post-hoc testing were performed. Results While COVID-19 cases with travel history to China seem to peak for each HAQ-cohort, the number of non-travel related COVID-19 cases seem to continuously increase in the HAQ-cohort of countries with higher medical standards. Further analyses demonstrate a significantly lower proportion of reported COVID-19 cases without travel history to China in countries with lower HAQ (HAQ I vs. HAQ II, posthoc p Conclusions Our data indicate that countries with lower HAQ-index may either underreport COVID-19 cases or are unable to adequately detect them. Although our data may be incomplete and must be interpreted with caution, inconsistencies in reporting COVID-19 cases is a serious problem which might sabotage efforts to contain the virus.

133 citations


Journal ArticleDOI
09 Sep 2020-Foods
TL;DR: This review reports the weaknesses of common drying methods applied for fruits, vegetables, and aromatic herbs and the possible options to improve the quality of dried products using different drying techniques or their combination.
Abstract: Drying is known as the best method to preserve fruits, vegetables, and herbs, decreasing not only the raw material volume but also its weight. This results in cheaper transportation and increments the product shelf life, limiting the food waste. Drying involves the application of energy in order to vaporize and mobilize the moisture content within the porous products. During this process, the heat and mass transfer occurs simultaneously. The quality of dehydrated fruits, vegetables, and aromatic herbs is a key problem closely related to the development and optimization of novel drying techniques. This review reports the weaknesses of common drying methods applied for fruits, vegetables, and aromatic herbs and the possible options to improve the quality of dried products using different drying techniques or their combination. The quality parameters under study include color, bulk density, porosity, shrinkage, phytochemicals, antioxidant capacity, sugars, proteins, volatile compounds, and sensory attributes. In general, drying leads to reduction in all studied parameters. However, the behavior of each plant material is different. On the whole, the optimal drying technique is different for each of the materials studied and specific conditions must be recommended after a proper evaluation of the drying protocols. However, a novel or combined technique must assure a high quality of dried products. Furthermore, the term quality must englobe the energy efficiency and the environmental impact leading to production of sustainable dried products.

110 citations


Journal ArticleDOI
TL;DR: An up-to-date overview on the main biochemical features and biotechnological applications of those reported enzymes which are able to degrade polyester-based plastics, including different microbial polyester depolymerases, esterases, cutinase-like enzymes and lipases.

89 citations


Journal ArticleDOI
TL;DR: This paper focuses on the cultivation methods of Haematococcus pluvialis, its chemical composition, extraction of astaxanthin and analysis of its antioxidant, anti-inflammatory, anti–diabetic and anticancer activities.
Abstract: Bioactive compounds of natural origin are gaining increasing popularity. High biological activity and bioavailability, beneficial effects on health and safety of use are some of their most desirable features. Low production and processing costs render them even more attractive. Microorganisms have been used in the food, medicinal, cosmetic and energy industries for years. Among them, microalgae have proved to be an invaluable source of beneficial compounds. Haematococcus pluvialis is known as the richest source of natural carotenoid called astaxanthin. In this paper, we focus on the cultivation methods of this green microalga, its chemical composition, extraction of astaxanthin and analysis of its antioxidant, anti-inflammatory, anti–diabetic and anticancer activities. H. pluvialis, as well as astaxanthin can be used not only for the treatment of human and animal diseases, but also as a valuable component of diet and feed.

77 citations


Journal ArticleDOI
TL;DR: In this article, the authors present the local approach for ecological footprint assessment and its potential to use for ECC assessment at local (i.e., city) level for the Polish city of Wroclaw in Central Eastern Europe for the year 2016.

75 citations


Journal ArticleDOI
TL;DR: It is demonstrated that higher lipid contents in the cells correlate with improved intracellular lycopene production, suggesting the importance of having a substantial hydrophobic environment to sequester isoprenoids.

60 citations


Journal ArticleDOI
TL;DR: A breeding investigation with various types of polystyrene was performed to follow the changes in the gut microbiome diversity, and the presence of a bacterial strain able to degrade bioplastic may suggest a potential niche for further investigations.

57 citations


Journal ArticleDOI
TL;DR: Based on available data on COVID‐19 cases of eight majorly affected countries, disease management in the 2 weeks following the first reported cases is of utmost importance and the opposite is true for European countries.
Abstract: With multiple virus epicenters, COVID-19 has been declared a pandemic by the World Health Organization. Consequently, many countries have implemented different policies to manage this crisis including curfew and lockdown. However, the efficacy of individual policies remains unclear with respect to COVID-19 case development. We analyzed available data on COVID-19 cases of eight majorly affected countries, including China, Italy, Iran, Germany, France, Spain, South Korea, and Japan. Growth rates and doubling time of cases were calculated for the first 6 weeks after the initial cases were declared for each respective country and put into context with implemented policies. Although the growth rate of total confirmed COVID-19 cases in China has decreased, those for Japan have remained constant. For European countries, the growth rate of COVID-19 cases considerably increased during the second time interval. Interestingly, the rates for Germany, Spain, and France are the highest measured in the second interval and even surpass the numbers in Italy. Although the initial data in Asian countries are encouraging with respect to case development at the initial stage, the opposite is true for European countries. Based on our data, disease management in the 2 weeks following the first reported cases is of utmost importance.

Journal ArticleDOI
TL;DR: The main functions of geotextiles are: separation, filtration, drainage, sealing, anti-erosion protection, and improving plant vegetation conditions as discussed by the authors, and they may be manufactured from synthetic or biodegradable materials.

Journal ArticleDOI
TL;DR: It has been stated that there is a positive correlation between caffeic acid derivatives and antioxidant activity (radical cation scavenging activity (ABTS) and radical scavenging activities (DPPH), especially in water-ethanolic extract of medicinal plants.
Abstract: Recently, there has been increasing interest in medicinal plants, due to their content of health-promoting compounds, e.g., caffeic acids derivatives. Hence, the aim of this work was to study the antioxidant activity of extracts obtained from the following medicinal plants: caraway (Carum carvi L.), coltsfoot (Tussilago farfara L.), dandelion (Taraxacum officinale F.H.Wigg.), lovage (Levisticum officinale L.), tarragon (Artemisia dracunculus L.) and white mulberry (Morus alba L.), characterized by their high content of caffeic acid derivatives. The water-ethanolic extracts were characterized on average by about 9 times higher contents of caffeic acid derivatives level than water extracts. Both in water and water-ethanolic extracts, the dominant phenolic acid was 5-CQA (5-O-caffeoylquinic acid) and 3,4-diCQA (3,4-dicaffeoylquinic acid), then CCA-1 (chicoric acid isomer 1), which appeared only in water-ethanolic extracts. Extracts from dandelion contained compounds such as CTA (caftaric acid), CCA-1 (chicoric acid isomer 1) and CCA-2 (chicoric acid isomer 2), which were not detected in other plant extracts examined in this work. The water-ethanolic extracts from coltsfoot and tarragon were characterized by a high content of di-caffeoylquinic acids, especially 3,4-diCQA and 3,5-diCQA, respectively. It has been stated that there is a positive correlation between caffeic acid derivatives and antioxidant activity (radical cation scavenging activity (ABTS) and radical scavenging activity (DPPH)), especially in water-ethanolic extract of medicinal plants.

Journal ArticleDOI
TL;DR: In this paper, the authors discuss the characteristics of peri-urban landscapes in the context of governance questions related to the management of ES trade-offs in the urban peripheries.

Journal ArticleDOI
TL;DR: In this article, the effect of the harvest date and rhubarb variety on the content of polyphenolic compounds was determined by liquid chromatography with quadrupole time-of-flight mass spectrometry.
Abstract: Rhubarb (Rheum spp.) is a plant characterized by very high antioxidant properties. It is very rich in many compounds that have a pro-health effect on the human body. Polyphenols are natural plant substances that can be a very important component in the prevention of various diseases. Therefore, the use of rhubarb as an interesting plant material in various industries has recently increased. The aim of the study was to determine the effect of the harvest date and rhubarb variety on the content of polyphenolic compounds. The quantitative determination of polyphenols was performed by liquid chromatography–mass spectrometry with quadrupole time-of-flight mass spectrometry. Rhubarb stalks of two Polish varieties Viktoria and Red Malinowy were used in the study. It was found that rhubarb is a source of flavan-3-ols (86.57–195.98 mg/100 gdm), flavonols (49.79–73.49 mg/100 gdm), anthocyanins (4.33–96.20 mg/100 gdm), and gallotannin (6.32–13.56 mg/100 gdm). Red Malinowy rhubarb from spring harvest was characterized by the highest and VA rhubarb was characterized by the lowest total antioxidant activity. It should be emphasized that the content of individual compounds depended on the variety and the date of harvest of rhubarb. Furthermore, the study revealed a high correlation between the polyphenol content and the total antioxidant capacity.

Journal ArticleDOI
TL;DR: In this article, a spatial analysis of ecosystem services (ES) in peri-urban areas is presented, where food and biomass production, water regulation, nutrient cycling, physical recreation, and aesthetic features of agricultural landscapes are analyzed.

Journal ArticleDOI
TL;DR: The results can be used to select conditions and strains in industrial-scale fermentation, to produce novel sea buckthorn products and increase their consumption.

Journal ArticleDOI
TL;DR: In this article, the authors proposed a methodology for estimating the PV potential in urban areas based on detailed Light Detection And Ranging (LiDAR) data and calculated the spatial variability of load and photovoltaics energy supply, and thus to distinguish zones with various levels of energy self-sufficiency.

Journal ArticleDOI
TL;DR: Several in vitro and in vivo studies have shown the activity of geraniol against prostate, bowel, liver, kidney and skin cancer, and the synergy of this with other drugs may further increase the range of chemotherapeutic agents.
Abstract: Geraniol is a monoterpenic alcohol with a pleasant rose-like aroma, known as an important ingredient in many essential oils, and is used commercially as a fragrance compound in cosmetic and household products. However, geraniol has a number of biological activities, such as antioxidant and anti-inflammatory properties. In addition, numerous in vitro and in vivo studies have shown the activity of geraniol against prostate, bowel, liver, kidney and skin cancer. It can induce apoptosis and increase the expression of proapoptotic proteins. The synergy of this with other drugs may further increase the range of chemotherapeutic agents. The antibacterial activity of this compound was also observed on respiratory pathogens, skin and food-derived strains. This review discusses some of the most important uses of geraniol.

Journal ArticleDOI
TL;DR: In this article, the replacement of synthetic colorants with natural ones has attracted increasing consumers' and market interest. Natural colorants include different groups of pigments, many of which are different types of colors.
Abstract: In recent years, the replacement of synthetic colorants with natural ones has attracted increasing consumers’ and market interest. Natural colorants include different groups of pigments, many of wh...

Journal ArticleDOI
13 Dec 2020
TL;DR: This review is to present possible ways to utilize common solid by-product from the brewing sector, which is a good material for sorption and processing into activated carbon and a waste rich in various substances that may be extracted for further utilization.
Abstract: The brewing sector is a significant part of the global food industry. Breweries produce large quantities of wastes, including wastewater and brewer's spent grains. Currently, upcycling of food industry by-products is one of the principles of the circular economy. The aim of this review is to present possible ways to utilize common solid by-product from the brewing sector. Brewer's spent grains (BSG) is a good material for sorption and processing into activated carbon. Another way to utilize spent grains is to use them as a fuel in raw form, after hydrothermal carbonization or as a feedstock for anaerobic digestion. The mentioned by-products may also be utilized in animal and human nutrition. Moreover, BSG is a waste rich in various substances that may be extracted for further utilization. It is likely that, in upcoming years, brewer's spent grains will not be considered as a by-product, but as a desirable raw material for various branches of industry.

Journal ArticleDOI
TL;DR: Results of this study show that sprouts are effective in antioxidant capacity as a result of a high content of polyphenols and L-ascorbic acid and are better sources of amino acids, pectins and sugars than microgreens.
Abstract: The aim of the study was to analyze potential health-promoting and nutritional components (polyphenols, L-ascorbic acid, carotenoids, chlorophylls, amino acids, organic acid, sugars, ash and pectins) of selected sprouts (radish, lentil, black medick, broccoli, sunflower, leek, beetroot, mung beans) and microgreens (kale, radish, beetroot, green peas, amaranth). Moreover, antioxidant capacity (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing ability of plasma (FRAP), and oxygen radical absorbance capacity (ORAC)), in vitro anti-diabetic potential (inhibition of α-amylase and α-glucosidase), and anti-obesity (pancreatic lipase) and anti-cholinergic (acetylcholinesterase and butyrylcholinesterase) activity were evaluated. The results of this study show that sprouts are effective in antioxidant capacity as a result of a high content of polyphenols and L-ascorbic acid. Additionally, sprouts are better sources of amino acids, pectins and sugars than microgreens. Microgreens were characterized by high content of carotenoids and chlorophylls, and organic acid, without any sugars, exhibiting higher anti-diabetic and anti-cholinergic activity than sprouts. Some selected sprouts (broccoli, radish, lentil) and microgreens (radish, amaranths, kale) should be used daily as superfoods or functional food.

Journal ArticleDOI
TL;DR: It is found that the most accurate orbits are currently available for GPS, however, Galileo utmost stable atomic clocks compensate for systematic errors in Galileo orbits, and the SISRE for Galileo is lower than that for GPS.
Abstract: High-quality satellite orbits and clocks are necessary for multi-GNSS precise point positioning and timing. In undifferenced GNSS solutions, the quality of orbit and clock products significantly influences the resulting position accuracy; therefore, for precise positioning in real time, the corrections for orbits and clocks are generated and distributed to users. In this research, we assess the quality and the availability of real-time CNES orbits and clocks for GPS, GLONASS, Galileo, and BeiDou-2 separated by satellite blocks and types, as well as the product quality changes over time. We calculate the signal-in-space ranging error (SISRE) as the main orbit and clock quality indicator. Moreover, we employ independent orbit validation based on satellite laser ranging. We found that the most accurate orbits are currently available for GPS. However, Galileo utmost stable atomic clocks compensate for systematic errors in Galileo orbits. As a result, the SISRE for Galileo is lower than that for GPS, equaling 1.6 and 2.3 cm for Galileo and GPS, respectively. The GLONASS satellites, despite the high quality of their orbits, are characterized by poor quality of clocks, and together with BeiDou-2 in medium and geosynchronous inclined orbits, are characterized by SISRE of 4–6 cm. BeiDou-2 in geostationary orbits is characterized by large orbital errors and the lowest availability of real-time orbit and clock corrections due to a large number of satellite maneuvers. The quality of GNSS orbit and clock corrections changes over time and depends on satellite type, block, orbit characteristics, onboard atomic clock, and the sun elevation above the orbital plane.

Journal ArticleDOI
TL;DR: The results obtained can be used to identify sea buckthorn cultivars, develop crops and production, and design functional products rich in flavonols and carotenoids with anticholinergic properties.

Journal ArticleDOI
TL;DR: Great potential benefits of this nutritious food are its ability to minimize the negative effects of UV radiation, diabetes mellitus and neurodegenerative diseases, and to exert hepato- and cardioprotective activity.
Abstract: Lonicera caerulea L., also known as haskap or honeysuckle berry, is a fruit commonly planted in eastern Europe, Canada and Asia. The fruit was registered as a traditional food from a third country under European Union regulations only on December 2018. It is resistant to cold, pests, various soil acidities and diseases. However, its attractiveness is associated mostly with its health properties. The fruit shows anticancer, anti-inflammatory, and antioxidant activity—important factors in improving health. These features result from the diverse content of phytochemicals in honeysuckle berries with high concentrations of phytocompounds, mainly hydroxycinnamic acids, hydroxybenzoic acids, flavanols, flavones, isoflavones, flavonols, flavanones and anthocyanins but also iridoids, present in the fruit in exceptional amounts. The content and health properties of the fruit were identified to be dependent on cultivar, genotype and the place of harvesting. Great potential benefits of this nutritious food are its ability to minimize the negative effects of UV radiation, diabetes mellitus and neurodegenerative diseases, and to exert hepato- and cardioprotective activity.

Journal ArticleDOI
TL;DR: In this paper, a qualitative photoproduction study based on the Checklist of Essential Features of Age-Friendly Cities was conducted in five neighbourhoods in the Dutch municipalities of The Hague and Zoetermeer.
Abstract: The World Health Organization (WHO) strives to assist and inspire cities to become more ‘age-friendly’ through the Global Age-Friendly Cities Guide. An age-friendly city offers a supportive environment that enables residents to grow older actively within their families, neighbourhoods and civil society, and offers extensive opportunities for their participation in the community. In the attempts to make cities age-friendly, ageism may interact with these developments. The goal of this study was to investigate the extent to which features of age-friendly cities, both facilitators and hindrances, are visible in the city scape of the Dutch municipalities of The Hague and Zoetermeer and whether or not ageism is manifested explicitly or implicitly. A qualitative photoproduction study based on the Checklist of Essential Features of Age-Friendly Cities was conducted in five neighbourhoods. Both municipalities have a large number of visual age-friendly features, which are manifested in five domains of the WHO model, namely Communication and information; Housing; Transportation; Community support and health services; and Outdoor spaces and buildings. Age-stereotypes, both positive and negative, can be observed in the domain of Communication and information, especially in the depiction of third agers as winners. At the same time, older people and age-friendly features are very visible in the cityscapes of both municipalities, and this is a positive expression of the changing demographics.

Journal ArticleDOI
TL;DR: Analysis of the volatile components in mango beer showed that beer without mango addition was characterized by the lowest content of volatile compounds, while beers with mango addition were characterized by a higher polyphenol content and antioxidant activity than control beer and were evaluated by a trained panel as having a better taste and aroma than beer without fruit addition.
Abstract: This study was performed to determine the possibility of using mango fruit (Mangifera indica) in brewing technology. The aim of using the SPME-HS-GC-MS technique was to assess what changes occurred in the volatile composition of mango beers brewed in this study. Mango fruit was added to the beer in five different forms to ascertain what kind of preparation should be used to improve beer aroma. Analysis of the volatile components in mango beer showed that beer without mango addition was characterized by the lowest content of volatile compounds (1787.84 µg/100 mL). The addition of mango fruit increased the concentration of compounds, such as α-pinene, β-myrcene, terpinolene, α-terpineol, cis-β-ocimene, caryophyllene, and humulene, in beer. Beer prepared with mango pulp addition was characterized by the highest concentration of volatile components from mango beers (2112.15 µg/100 mL). Furthermore, beers with mango addition were characterized by a higher polyphenol content (up to 44% higher than control beer) and antioxidant activity than control beer and were evaluated by a trained panel as having a better taste and aroma than beer without fruit addition.

Journal ArticleDOI
TL;DR: One important outcome of the workshop was the creation of the Equine Asthma Group that will facilitate communication between veterinary practice and research communities through published and easily accessible guidelines and foster research collaboration.
Abstract: The 2019 Havemeyer Workshop brought together researchers and clinicians to discuss the latest information on Equine Asthma and provide future research directions. Current clinical and molecular asthma phenotypes and endotypes in humans were discussed and compared to asthma phenotypes in horses. The role of infectious and non-infectious causes of equine asthma, genetic factors and proposed disease pathophysiology were reviewed. Diagnostic limitations were evident by the limited number of tests and biomarkers available to field practitioners. The participants emphasized the need for more accessible, standardized diagnostics that would help identify specific phenotypes and endotypes in order to create more targeted treatments or management strategies. One important outcome of the workshop was the creation of the Equine Asthma Group that will facilitate communication between veterinary practice and research communities through published and easily accessible guidelines and foster research collaboration.

Journal ArticleDOI
TL;DR: Recipes for traditional and sour non-alcoholic beers developed in this study employing a special yeast species Saccharomycodes ludwigii had several times higher antioxidative potential and significantly higher polyphenols concentration compared to the control ones.

Journal ArticleDOI
TL;DR: Calcium and copper ions were encapsulated in biopolymeric microparticles either as single agents or in combination with biocontrol agents, Trichoderma viride spores, a fungal plant growth mediator to stimulate the production of plant secondary metabolites increasing its nutritive value.
Abstract: Encapsulated bioactive agents applied to the Lactuca sativa L. present an innovative approach to stimulate the production of plant secondary metabolites increasing its nutritive value. Calcium and copper ions were encapsulated in biopolymeric microparticles (microspheres and microcapsules) either as single agents or in combination with biocontrol agents, Trichoderma viride spores, a fungal plant growth mediator. Both, calcium and copper ions are directly involved in the synthesis of plant secondary metabolites and alongside, Trichoderma viride can provide indirect stimulation and higher uptake of nutrients. All treatments with microparticles had a positive effect on the enhancement of plant secondary metabolites content in Lactuca sativa L. The highest increase of chlorophylls, antioxidant activity and phenolic was obtained by calcium-based microparticles in both, conventionally and hydroponically grown lettuces. Non-encapsulated fungus Trichoderma viride enhanced the synthesis of plant secondary metabolites only in hydroponics cultivation signifying the importance of its encapsulation. Encapsulation proved to be simple, sustainable and environmentally favorable for the production of lettuce with increased nutritional quality, which is lettuce fortified with important bioactive compounds.