Xuzhou Medical College

About: Xuzhou Medical College is a education organization based out in Xuzhou, China. It is known for research contribution in the topics: Cancer & Cell growth. The organization has 12721 authors who have published 7802 publications receiving 102970 citations.

The circular RNA circSPARC enhances the migration and proliferation of colorectal cancer by regulating the JAK/STAT pathway

Abstract: Noncoding RNAs such as circular RNAs (circRNAs) are abundant in the human body and influence the occurrence and development of various diseases However, the biological functions of circRNAs in colorectal cancer (CRC) are largely unknown RT-qPCR was used to detect the expression of circRNAs and mRNA in CRC cells and tissues Fluorescence in situ hybridization (FISH) was used to analyze the location of circSPARC Function-based experiments were performed using circSPARC knockdown and overexpression cell lines in vitro and in vivo, including CCK8, colony formation, transwell and metastasis models Mechanistically, luciferase reporter assay, western blots, RNA immunoprecipitation (RIP), Chromatin isolation by RNA purification (ChIRP) and immunohistochemical stainings were performed CircSPARC was upregulated in both the tissues and plasma of CRC patients High expression of circSPARC was associated with advanced TNM stage, lymph node metastases, and poor survival Silencing circSPARC inhibited CRC cell migration and proliferation in vitro and vivo Mechanistically, circSPARC sponged miR-485-3p to upregulate JAK2 expression and ultimately contribute to the accumulation of phosphorylated (p)-STAT3 Besides, circSPARC recruited FUS, which facilitated the nuclear translocation of p-STAT3 These findings suggest that circSPARC might serve as a potential diagnostic and prognostic biomarker and a therapeutic target for CRC treatment by regulating JAK2/STAT3 pathway

A Method for Evaluating the Level of Soluble β-Amyloid (1--40/1--42) in Alzheimer’s Disease Based on the Binding of Gelsolin to β-Amyloid Peptides

Abstract: In the present work, a new electrochemical strategy for the sensitive and specific detection of soluble β-amyloid Aβ(1-40/1-42) peptides in a rat model of Alzheimer's disease (AD) is described In contrast to previous antibody-based methods, β-amyloid(1-40/1-42) was quantified based on its binding to gelsolin, a secretory protein present in the cerebrospinal fluid (CSF) and plasma The level of soluble β-amyloid peptides in the CSF and various brain regions were found with this method to be lower in rats with AD than in normal rats

Electrospun Core–Shell Fibrous 2D Scaffold with Biocompatible Poly(Glycerol Sebacate) and Poly-l-Lactic Acid for Wound Healing

Abstract: Biomimetic scaffolds made by synthetic materials are usually used to replace the natural tissues aimed at speeding up the skin regeneration. In this study, a flexible and cytocompatible poly(glycerol sebacate)@poly-l-lactic acid (PGS@PLLA) fibrous scaffold with a core–shell structure was fabricated by coaxial electrospinning, where the shell PLLA was used to be a skeleton with pores on the fibrous surface. The fibrous morphology with pores on the surface of the prepared fibers was observed by SEM. The core–shell microstructure of PGS@PLLA fibers was confirmed by TEM and Laser Scanning Confocal Microscopy (LSCM). In addition, the prepared fibers exhibited a strong ability to repair tissues of the skin wound, where the stability of cell security and proliferation, and the lower inflammatory response were all superior to those of pure PLLA scaffold. It’s worth noting that the percentage of skin tissue was regenerated by 95% within 14 days, which suggests the potential application for electrospun-based synthetic fibrous scaffolds on wound healing.

A preliminary analysis of microRNA as potential clinical biomarker for schizophrenia

Abstract: MicroRNAs (miRNA, miR) have been implicated as promising blood-based biomarkers for schizophrenia patients This study aimed to clinically validate miRNA as potential schizophrenia biomarkers Plasma levels of 10 miRNAs were analyzed using qPCR in a cohort of 61 schizophrenia patients and 62 normal controls, as well as 25 patients particularly selected for a six-week antipsychotic treatment course Positive And Negative Syndrome Scale (PANSS), Global Assessment Scale (GAS) and Clinical Global Impression (CGI) were administered to assess the clinical symptoms The results demonstrated that a panel of miRNAs consisting of miR-30e, miR-181b, miR-34a, miR-346 and miR-7 had significantly increased expression levels with significant combined diagnostic value (AUC:0713; sensitivity:355%; specificity:902%) In response to pharmacological treatment, expression levels of miR-132, miR-181b, miR-432 and miR-30e were significantly decreased In addition, the improvement of clinical symptomatology was significantly correlated with the changes of miR-132, miR-181b, miR-212 and miR-30e expression levels Furthermore, the decreases of plasma levels of miR-132 and miR-432 were significantly greater in high-effect subgroup than those in low-effect subgroup after six-week treatment course We conclude that miR-30e, miR-181b, miR-34a, miR-346 and miR-7 combined as a panel are potentially useful non-invasive biomarkers for schizophrenia diagnosis Markers miR-132, miR-181b, miR-30e and miR-432 are potential indicators for symptomatology improvements, treatment responses and prognosis for schizophrenia patients