Institution
Xuzhou Medical College
Education•Xuzhou, China•
About: Xuzhou Medical College is a education organization based out in Xuzhou, China. It is known for research contribution in the topics: Cancer & Cell growth. The organization has 12721 authors who have published 7802 publications receiving 102970 citations.
Topics: Cancer, Cell growth, Apoptosis, Medicine, Protein kinase B
Papers published on a yearly basis
Papers
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TL;DR: A new multifunctional probe comprising a cell-specific internalization aptamer, fluorescent silver nanoclusters (Ag NCs), and therapeutic siRNA was developed in one system for the specific delivery of siRNA into a target cell and for simultaneous noninvasive imaging.
Abstract: The use of small interfering RNA (siRNA) to silence target genes involved in disease has generated much excitement in the scientific community. While promising, the clinical application of RNA interference (RNAi) is still challenging in achieving effective delivery and tracking of siRNA to target cells. A new multifunctional probe comprising a cell-specific internalization aptamer, fluorescent silver nanoclusters (Ag NCs), and therapeutic siRNA was developed in one system for the specific delivery of siRNA into a target cell and for simultaneous noninvasive imaging. Different from described nanocarrier-based delivery methods which have to suffer from complicated conjugation, Ag NCs could be synthesized directly from the aptamer chimera. Sgc8c aptamer-functionalized Ag NCs as a cell-type specific siRNA delivery and imaging probe complements recent advances in PSMA aptamer-based siRNA delivery and nanomaterial-based molecular imaging. Besides, siRNA in the Ag NCs–streptavidin–siRNA complex displayed outstanding stability in both binding buffer and cell culture medium. The fluorescent intensity of biotinylated aptamer-functionalized Ag NCs was enhanced in acidic environment and no observable quenching of fluorescence occurred even after incubation for 48 h, which could benefit their usage in the intracellular environment. The facile synthetic process, good biocompatibility, excellent stability and comparable gene silencing effect with commercial reagent make it more promising for in vivo applications.
44 citations
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TL;DR: Tumor targeting in vivo studies clearly demonstrated DOX@MSNs-CAIX accumulated in tumors and induced more tumor cells apoptosis in 4T1 tumor-bearing mice.
Abstract: In this work, we developed a new antibody-targeted and redox-responsive drug delivery system "MSNs-CAIX" by binding the anti-carbonic anhydrase IX antibody (A-CAIX Ab) on the surface of mesoporous silica nanoparticles (MSNs) via disulfide linkages. The design of the composite particles "MSNs-CAIX" involved the synthesis and surface functionalization with thiol groups, 2,2'-dipyridyl disulfide and CAIX antibody. In vitro, CAIX capping the doxorubicin hydrochloric (DOX)-loaded nanoparticles (DOX@MSNs-CAIX) exhibited effectively redox-responsive release in the presence of glutathione (GSH) owing to the cleavage of the disulfide bond. Compared with CAIX negative Mef cells (mouse embryo fibroblast), remarkably more DOX@MSNs-CAIX was internalized into CAIX positive 4T1 cells (mouse breast cancer cells) by receptor-mediation. Tumor targeting in vivo studies clearly demonstrated DOX@MSNs-CAIX accumulated in tumors and induced more tumor cells apoptosis in 4T1 tumor-bearing mice. With great potential, this drug delivery system is a promising candidate for targeted and redox-responsive cancer therapy.
44 citations
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TL;DR: It is revealed that utilizing PEI-GA/DOX/shAkt1 complexes results in effective autophagy and apoptosis, which are useful to cause cell death, which is meaningful for oncotherapy.
Abstract: Combination therapy has been developed as a promising therapeutic approach for hepatocellular carcinoma therapy. Here we report a low toxicity and high performance nanoparticle system that was self-assembled from a poly(ethylenimine)-glycyrrhetinic acid (PEI-GA) amphiphilic copolymer as a versatile gene/drug dual delivery nanoplatform. PEI-GA was synthesized by chemical conjugation of hydrophobic GA moieties to the hydrophilic PEI backbone via an acylation reaction. The PEI-GA nanocarrier could encapsulate doxorubicin (DOX) efficiently with loading level about 12% and further condense DNA to form PEI-GA/DOX/DNA complexes to codeliver drug and gene. The diameter of the complexes is 102 ± 19 nm with zeta potential of 19.6 ± 0.2 mV. Furthermore, the complexes possess liver cancer targeting ability and could promote liver cancer HepG2 cell internalization. Apoptosis of cells could be induced by chemotherapy of DOX, and PI3K/Akt/mTOR signaling pathway acts a beneficial effect on the modulation of autophagy. Here, it is revealed that utilizing PEI-GA/DOX/shAkt1 complexes results in effective autophagy and apoptosis, which are useful to cause cell death. The induction of superfluous autophagy is reported to induce type-II cell death and also could increase the sensity of chemotherapy to tumor cells. In this case, combining autophagy and apoptosis is meaningful for oncotherapy. In this study, PEI-GA/DOX/shAkt1 has demonstrated favorable tumor target ability, little side effects, and ideal antitumor efficacy.
43 citations
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TL;DR: It is concluded that the dopamine receptor 1-dependent disruption of FS GABAergic inhibitory input plays a critical role in Aβ-induced excitation/inhibition imbalance in anterior cingulate cortex.
Abstract: Alzheimer’s disease (AD) is the most common cause of dementia in the elderly. At the early stages of AD development, the soluble β-amyloid (Aβ) induces synaptic dysfunction, perturbs the excitation/inhibition balance of neural circuitries, and in turn alters the normal neural network activity leading to cognitive decline, but the underlying mechanisms are not well established. Here by using whole-cell recordings in acute mouse brain slices, we found that 50 nM Aβ induces hyperexcitability of excitatory pyramidal cells in the cingulate cortex, one of the most vulnerable areas in AD, via depressing inhibitory synaptic transmission. Furthermore, by simultaneously recording multiple cells, we discovered that the inhibitory innervation of pyramidal cells from fast-spiking (FS) interneurons instead of non-FS interneurons is dramatically disrupted by Aβ, and perturbation of the presynaptic inhibitory neurotransmitter gamma-aminobutyric acid (GABA) release underlies this inhibitory input disruption. Finally, we identified the increased dopamine action on dopamine D1 receptor of FS interneurons as a key pathological factor that contributes to GABAergic input perturbation and excitation/inhibition imbalance caused by Aβ. Thus, we conclude that the dopamine receptor 1-dependent disruption of FS GABAergic inhibitory input plays a critical role in Aβ-induced excitation/inhibition imbalance in anterior cingulate cortex.
43 citations
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TL;DR: As results, SPUP can significantly restrain breast abnormal enlargement, prolong tumor latency and reduced tumor incidence and the strongly against breast cancer activity of S PUP was confirmed by DMBA-induced breast cancer rats model.
43 citations
Authors
Showing all 12775 results
Name | H-index | Papers | Citations |
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Liang Wang | 98 | 1718 | 45600 |
Chang Liu | 97 | 1099 | 39573 |
Wei Wang | 95 | 3544 | 59660 |
Yu Liu | 66 | 1262 | 20577 |
Deling Kong | 65 | 388 | 16515 |
Zhimou Yang | 61 | 222 | 12522 |
Xu-Feng Huang | 61 | 332 | 13074 |
Guangming Lu | 60 | 476 | 13218 |
Dan Ding | 59 | 212 | 12494 |
Jian Cao | 58 | 486 | 11074 |
Yuanjin Zhao | 57 | 328 | 12076 |
Jie Yang | 56 | 488 | 11382 |
Lei Wang | 54 | 1076 | 15189 |
Xiaodong Shi | 52 | 323 | 8910 |
Wei Pan | 50 | 408 | 9037 |