Showing papers in "Biomolecular Engineering in 2001"

TL;DR: In this article, the introduction of two transgenes into one animal is increasingly common as transgenic experiments become more sophisticated, and the authors examined two strategies for creating double transgenic founders from a single microinjection.

TL;DR: There are a number of methods that can be used for the preparation of enzyme-containing lipid vesicles (liposomes) which are lipid dispersions that contain water-soluble enzymes in the trapped aqueous space, and a review of these studies is given and some of the main results are summarized.

TL;DR: The discovery of more potent adjuvants may allow the development of vaccines against infectious agents such as HIV which do not naturally elicit protective immunity and may also allow vaccines to be delivered mucosally.

TL;DR: A number of cancer-targeting scFv diabodies that have undergone successful pre-clinical trials for in vivo stability and efficacy are highlighted.

TL;DR: It is demonstrated that the M13 phage coat is extremely malleable, and this property can be used to engineer the phage particle specifically for phage display, expanding the utility of phagedisplay as a powerful tool in modern biotechnology.

TL;DR: In this article, the four major methods for gas chromatography-olfactometry are described and their potentials and limitations discussed, including dilution analysis, detection frequency methods, posterior intensity methods and time-intensity methods.

TL;DR: In the wake of recent advances in mass spectrometry and related areas, areas where two-dimensional gels can best be utilized as the preferred separation method in proteomic strategies are outlined.

TL;DR: Clinical trials in humans of recombinant bispecific and bifunctional antibodies, as a new generation of biologicals, are likely to be the thrust in the next decade and beyond.

TL;DR: This overview emphasizes some important aspects for the control of lipase enantioselectivity and some examples where the enantiOSElectivity has been altered or reversed are highlighted.

TL;DR: The progressive improvement of expression vectors for plantibodies, and purification strategies, is expected to lead to almost limitless availability of inexpensive (even edible forms of) recombinant immunoglobulins free of human pathogens for human and animal therapy, and for novel industrial applications.

TL;DR: Biocatalytic processes were used to prepare chiral intermediates required for the synthesis of Omapatrilat 1 by three different routes and Phenylalanine dehydrogenase was cloned and overexpressed in Escherichia coli and Pichia pastoris.

TL;DR: First in vitro experiments to specifically down regulate protein expression by PNA have been followed by successful antisense and antigene application of PNA oligomers in vivo.

TL;DR: It is shown here that a further barrier to efficient and sustained expression exists for synthetic vectors: plasmid DNA methylation.

TL;DR: By circumventing inhibiting product concentrations and thus intensifying aroma production, total yield of aroma compounds produced is higher in an IBP compared with batch cultivation and permeates obtained from pervaporation consist of highly enriched mixtures of produced flavors and fragrances.

TL;DR: It was revealed that bacterial spores, sodium alginate and bacterial cellulose accelerated hardening of the cryogels and modified their porosity.

TL;DR: The biotransformation of morphine and codeine to the potent analgesic hydromorphone and the mild analgesic/antitussive hydrocodone, respectively, by recombinant Escherichia coli has been demonstrated and the problems encountered when engineering such a system will be discussed.

TL;DR: The recent developments and applications in proteomics are discussed including mass spectrometry data analysis and interpretation, analysis and storage of the gel images to databases, gel comparison, and advanced methods to study e.g. protein co-expression, protein-protein interactions, as well as metabolic and cellular pathways.

TL;DR: This approach can identify promoter modules responsible for the common regulation of promoters solely by the application of bioinformatics methods and provides a powerful alternative for elucidating functional features of genes with no detectable sequence similarity by linking them to other genes on the basis of their common promoter structures.

TL;DR: Novel mass spectrometric, affinity and display techniques are discussed for their use for the detection of posttranslational modifications, functional interactions and possible disease-associated abnormalities in proteins.

TL;DR: It is demonstrated that the catalysts can be utilized not only in batch but also in continuously driven reactors and that their performance can be improved by means of chemical reaction engineering.

TL;DR: It was found that the isolated CL:CH1 domain interaction was inefficient for secretion of heterodimers, however, when the complete Fab chains were used, secretion of a heterodimerized bispecific antibody was successful.

TL;DR: Annexin V was radiolabelled with iodine-123 in order to develop a SPECT-ligand for imaging atherosclerosis and apoptosis to demonstrate its biological activity.

TL;DR: Flavour release was investigated from pure gelatin, pure agarose and mixed gelatin-agarose gels, all containing 25% sucrose and flavoured with p-cymene, ethyl butyrate, pyrazine and ethanol suggesting both the properties of the volatile and the matrix determine volatile release in vivo.

TL;DR: The potential of molecular characterization of MRCC as a tool to improve prognostication, therapy selection and drug targeting as well as therapy monitoring is discussed.

TL;DR: The current MS methods used in proteome studies are introduced, and their advantages and disadvantages are discussed, and new instrumental MS developments are presented that are useful in these analyses.

TL;DR: The techniques of release cells and breath analysis must now be applied to provide data, which will allow flavour release to be modelled and determine the extent to which there are real differences in volatile profiles.

TL;DR: The most widely used bacterial carrier systems for either antigens or nucleic acid vaccines, and the strategies which have been successfully exploited to modulate the immune responses elicited are discussed.

TL;DR: Biological systems are comprised of protein components found at a wide variety of abundances from millions of molecules of a single species per cell to less than one copy per cell, and analytical procedures can be devised and applied to complex mixtures.

TL;DR: A species of facultative photo-organotrophic, purple, non-sulfur bacterium isolated from mixed-species microbial mats, characterized and examined for metal tolerance and bioremediation potential may be identified as a new strain of the genus Rhodopseudomonas.

TL;DR: It was found that the hydrogen bonding between the chemisorbed OH-group and the solvent was enhanced in UMF's and reduced in CMF's.