Showing papers in "Biosensors and Bioelectronics in 1999"

TL;DR: An overview of different physicochemical instrumental techniques for direct and indirect identification of bacteria such as: infrared and fluorescence spectroscopy, flow cytometry, chromatography and chemiluminescence techniques as a basis for biosensor construction is presented.

TL;DR: The construction of controlled architectures based on spatially segregated multilayers, exhibiting complementary biological activities is described, and the attachment of biomolecules to biotinylated polymers through affinity interactions based on avidin-biotin bridge is presented.

TL;DR: A tutorial review article on the quartz crystal microbalance (QCM) is presented in this paper, followed by a summary of recent and important prior research in each of the different areas of analytical interest in the QCM.

TL;DR: In this article, a qualitative evaluation of eight chemical methods commonly used to clean glass microscope slides in preparation for silanization was presented, and the mean contact angle of deionized water was measured before covalent attachment of (3-mercaptopropyl)triethoxysilane to assess the efficacy of each procedure.

TL;DR: Systematic evolution of ligands by exponential enrichment was used to select and PCR amplify DNA sequences (aptamers) capable of binding to and detecting nonpathogenic Sterne strain Bacillus anthracis spores.

TL;DR: In this article, a 3D microelectrode system consisting of two layers of electrode structures separated by a 40 μm thick polymer spacer forming a flow channel was designed to focus, trap and separate eukaryotic cells (Jurkat) or latex particles using negative dielectrophoresis (nDEP).

TL;DR: In this article, a disposable electrochemical sensor for the detection of short DNA sequences is described, where synthetic single-stranded oligonucleotides have been immobilized onto graphite screen printed electrodes with two procedures, the first involving the binding of avidin-biotinylated oligonotide and the second adsorption at a controlled potential.

TL;DR: A potentiometric enzyme electrode for the direct measurement of organophosphate (OP) nerve agents was developed and the best sensitivity and response time were obtained using a sensor constructed with 500 IU of OPH and operating in pH 8.5, 1 mM HEPES buffer.

TL;DR: A flow-through amperometric immunofiltration assay system based on disposable porous filter-membranes for rapid detection of Escherichia coli O157:H7 has been developed and may be a basis for a new class of highly sensitive bioanalytical devices for rapid quantitative detection of bacteria.

TL;DR: The experiments demonstrate the possibility to assess the activity of integral membrane proteins incorporated in peptide tBLMs using electrochemical techniques and could be promising for screening as well as biosensor applications.

TL;DR: A sol-gel process was used to create highly porous and stable silica matrices to create microsensors with increased surface area for enzyme immobilization and significantly improved long term stability of enzyme activity.

TL;DR: A novel ISFET-based measurement concept for the determination of the zeta potential, which is known to be an efficient method for the detection of protein accumulations onto surfaces, and the feasibility of an immunosensor based on the measurement of the streaming potential is demonstrated.

TL;DR: The phersomone oriented behavior of male silkworm moths was shown by a pheromone-guided mobile robot which had male moth antennae that can detect sex pherOMones.

TL;DR: This paper describes a generally applicable method using a synthetic chelator thioalkane that can be self-assembled on gold surfaces that opens possibilities for the development of biosensors.

TL;DR: An array of six sensors consisting of five fractionated ORPs and one referenced phospholipid probe is able to respond to different odorants and form a typical fingerprint for each odorant.

TL;DR: The combination of an integrated Mach-Zehnder-interferometer (iMZI) at the bottom of a fluidic microchannel system with supramolecular interfacial binding layers optimized for biosensing purposes is described.

TL;DR: The detailed design and characterisation of a regenerable integrated optical surface plasmon resonance immunoprobe as a detector for the triazine herbicide simazine is presented and its fabrication described.

TL;DR: A potentiometric urea-sensitive biosensor using a NH4(+)-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer and a good correlation was found with the data obtained by the spectrophotometric routine method.

TL;DR: The DNA detection system, the combination of BIAcore and the probe PNA, could detect the target DNA with good reproducibility and is shown to be a powerful tool for the diagnosis of pathologically significant DNA.

TL;DR: 1-NP possesses the advantages over 4-APP of being inexpensive, easy to obtain and soluble (1-naphthol cf. 4-aminophenol) at high pH.

TL;DR: A bienzyme amperometric graphite-Teflon composite biosensor, in which lactate oxidase and peroxidase are incorporated into the electrode matrix, was developed for the determination of L-lactate in food samples such as wine and yogurt by using both batch- and flow-injection modes.

TL;DR: The Man-portable Analyte Identification System (MANTIS), the first fully automated, self-contained, portable fiber optic biosensor, was utilized for the detection of Staphylococcal Enterotoxin B (SEB), a bacterial toxin produced by Staphylon aureus that commonly causes food poisoning.

TL;DR: The determination of organophosphate (paraoxon, chlorpyrifos, diazinon) and carbamate (carbaryl, carbofuran) pesticides in spiked drinking water and fruit juices was carried out using a photothermal biosensor using a cartridge containing immobilised enzyme acetylcholinesterase placed in a flow-injection analysis (FIA) manifold and aPhotothermal detector based on thermal lens spectrometry.

TL;DR: A biosensor on the basis of intact insect antennae of the Colorado potato beetle presents a solution to the problem of damageless screening of large numbers of seed potatoes for fungal infestation.

TL;DR: Glucose-sensitive enzyme field effect transistors modified by an additional Nafion membrane have been developed and used for diluted blood samples analysis and good correlation between these methods was revealed.

TL;DR: In this paper, a tethered mediator was used to build an electronic interface between a redox enzyme and an electrode, where the mediator molecule was immobilized onto an electrode surface via a long flexible chain.

TL;DR: P pH-sensitive field effect transistors (FET) were modified with organophosphate hydrolase (OPH) and used for direct detection of organoph phosphate compounds and successful reuse of sensor chips was demonstrated.

TL;DR: The sensor sensitivity to B2M increased with increasing flexibility of the antibody networks and with increasing number of anti-B2M molecular layers, indicating that B 2M can penetrate inside the antibody network.

TL;DR: A microbial biosensor based on the yeast Arxula adeninivorans LS3 has been developed for measurement of biodegradable substances and has a very high stability which can last for 40 day without any decrease in signal.

TL;DR: Application to many different areas, including an early warning system of wastewater biotreatment plant upsets and the monitoring and tracking of accidental spills, discharges or failures in plant operation are plausible.