Showing papers in "Cell Biology International in 2011"

TL;DR: The results demonstrated molecular mechanism of eugenol‐induced apoptosis in human colon cancer cells and will further enhanceeugenol as a potential chemopreventive agent against colon cancer.

TL;DR: A new and efficient approach in generating MSC from UC is demonstrated, hence serving as ideal alternative source of mesenchymal stem cell for clinical and research use.

TL;DR: ‘neotic’ ETC resulting from genotoxically damaged p53 function‐deficient tumour cells develop through a heteronuclear system differentiating the polyploid genome into rejuvenated ‘viable’ subcells and subnuclei, which become degraded and eliminated by autophagy.

TL;DR: The results suggest that hypoxia negatively affects MSC migration by regulating activation of GTPases, and highlight the importance of oxygen in regulating the recruitment of progenitor cells to areas of ischaemic tissue damage.

TL;DR: In this paper, a new approach to differentiate the MSCs towards the myogenic lineage, co-cultivation with primary myoblasts has been developed, and myogenic potential of GFP (green fluorescent protein)-transduced rat mesenchymal stem cells (MSC) was assessed by ICC (immunocytochemistry) and FACS (quantitative PCR).

TL;DR: It is reported that osteosarcoma‐derived MICA results from proteolytic cleavage of MICA α3 ectodomain, which facilitates tumour immune escape and using a specific MMP inhibitor might represent a double‐edged sword, where it can inhibit tumour invasion and restore antitumour immune response.

TL;DR: It is concluded that chimeric gene reporter approaches, though powerful, should be applied cautiously, due principally to GFP (or other reporter tag) interference with protein organelle targeting or incorporation into macromolecular assemblies, both of which cause spuriously high degradation rates.

TL;DR: It is demonstrated that BMSCs can be successfully differentiated into type II alveolar epithelial cells in vitro under mimic pulmonary microenvironment and showed that the SPC gene expression level in differentiated cells was related to the ratio of B MSCs to MRC‐5 cells and the components of modified SAGM.

TL;DR: HPL offers a promising alternative to FCS for MSC expansion for clinical application, especially in bone injury diseases and showed an increase in osteogenic differentiation and a decrease in adipogenic differentiation compared with FCS group.

TL;DR: It is important that differences between satellite cell‐derived myoblasts and cell line C2C12 grown in monolayer and 3D cultures are taken into account when studying processes of myoblast differentiation in vitro.

TL;DR: The cells have the characteristics of cancer stem‐like cells, and identification and cloning of such human SP cells can help in improving therapeutic approaches to ovarian cancer in patients.

TL;DR: TQ in combination with single dose of ionizing radiation was found to exert supra‐additive cytotoxic effects on both the carcinomas as measured by cell proliferation and colony‐formation assays, supporting TQ as an adjuvant for preclinical testing in cancer chemo‐radiotherapy.

TL;DR: A role for hypoxia and the induction of HIF‐1α (hypoxia‐inducible factor 1α) in increasing renal primary cilium length following renal injury is suggested.

TL;DR: The effects of NSAIDs on MSCs are largely dependent on the concentrations used, and for some differentiation lineages, also the choice of NSAID is critical.

TL;DR: It is confirmed that CXCR4 silencing via subcutaneous injection could reduce tumour growth as well as inhibit metastasis, particularly bone metastasis; and the expression of VEGF and MMP‐9 were reduced by the knockdown of CX CR4 in the primary tumours of mice.

TL;DR: Results indicate that KA can modulate macrophage activation through cytoskeleton rearrangement, increase cell surface exposure, enhance the phagocytic process and ROS production, and demonstrates a new role for KA as a macrophages activator.

TL;DR: The results strongly suggest that the proliferation and migration of EPCs may depend on bFGF and/or PDGF‐BB by PDGFRβ/PLC‐γ signalling pathway, and bF GF and/ or PD GF‐BB stimulate VEGF release at a point downstream from PDG FRβ/C gamma in EPC's.

TL;DR: It is demonstrated that NK‐1 receptor and SP‐containing nerves existed in human gastric cancer; SP positive nerves may play an important role in human Gastric cancer progression, and calcium is critically significant among SP‐induced biological effects.

TL;DR: It is concluded that the critical determinants of EC network formation and cell survival are not universal, but have to be specifically optimized for each culture model.

TL;DR: Very high expression of microRNAs and their target genes are very likely to play important regulatory roles in the development of pancreas and/or differentiation of islet cells, and they may be manipulated to increase the proportion of beta cells and insulin synthesis in the differentiated T3pi for cell therapy of type I diabetics.

TL;DR: Results indicate MSC rescue neutrophils from nutrient‐ or serum‐deprived cell death, however, whether this effect is exerted through a specific signalling pathway or confining neutrophil in resting state by MSC requires further investigation.

TL;DR: An increase in ovarian apoptosis through an imbalance among the Bcl‐2 family members may be involved in the transformation of growing follicle in cystic follicles in the ovaries from DHEA‐induced PCOS rats.

TL;DR: The hypothesis that combining the inhibition of melanogenesis with PDT should be explored as a valid therapeutic target for the management of advanced melanoma is supported.

TL;DR: This work has found that the recombinant bacteria, whose extracts limitedly cleaved actin were internalized within the eukaryotic cells residing both in vacuoles and free in cytoplasm, consistent with the direct participation of ECP32/grimelysin and protealysin in entry of bacteria into the host cells.

TL;DR: By employing optimum culture conditions, it is possible to enhance the production of secondary compounds from the cells and the factors optimized for in vitro production of shikonin derivatives can successfully be employed for their large‐scale production in bioreactors.

TL;DR: It is suggested that myostatin suppressed 3T3‐L1 preadipocyte differentiation and regulated lipid metabolism of mature adipocyte, in part, via activation of ERK1/2 signalling pathway.

TL;DR: The results suggest that the disruption of actin skeleton is positive in protecting cells from killing by electric pulses, and the skeleton is not involved in the electroporation directly.

TL;DR: Common conclusion concerns compatibility of ND with living neutrophils from inflammatory site and their normal functioning for infection safeguard and the various mechanisms that could account for penetration of ND particles into the cell are discussed.

TL;DR: It is suggested that MWCNT‐coated nanofibrous scaffolds may be an attractive platform for cell transplantation application in neural tissue engineering.

TL;DR: Current theories accounting for the disease variations are discussed, an epigenetic hypothesis involving microRNAs is put forth and large variation in disease phenotypes and severity is observed among the patients.