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Showing papers in "Electronic Journal of Biotechnology in 2011"


Journal ArticleDOI
TL;DR: In this article, the authors highlight the impact of biocatalysis within a platform of whey upgrading, with a special focus on newly developed enzymatic processes of organic synthesis where the added value is high, being then a powerful driving force for industrial implementation.

85 citations


Journal ArticleDOI
TL;DR: An optimized and modified total RNA isolation method was developed for recalcitrant mangrove species and resulted in high quantity and quality RNA, suitable for cDNA synthesis, RT-PCR experiments and the phytochelatin synthase 1 gene amplification.

79 citations


Journal ArticleDOI
TL;DR: The evaluation of genetic similarity and cluster analysis together with salt tolerance ability provides some useful guides for assisting plant breeders in selecting suitable genetically diverse parents for the crossing program.

69 citations


Journal ArticleDOI
TL;DR: The data suggest that combinations including seedling resistance genes like Lr16, Lr47, LR19, L r41, Ll21, Lm21, lr41, and Lr21, with adult plant resistance genes such as Lr34, SV2,Lr46 will probably provide durable and effective resistance to leaf rust in the region.

67 citations


Journal ArticleDOI
TL;DR: Two purple nonsulfur bacteria strains, Rhodobium marinum NW16 and Rhodobacter sphaeroides KMS24, were investigated for their potential to remove heavy metals from contaminated shrimp pond water and altered the cellular morphology of both strains and accumulated HMs were found in their cells.

59 citations


Journal ArticleDOI
TL;DR: In this paper, the optimal conditions for producing functional fermented red seaweed beverage (FSB) were established using Central Composite Design by varying the amounts of monosodium glutamate (MSG), sucrose and the initial pH in MRS medium.

56 citations


Journal ArticleDOI
TL;DR: Lipase production was the result of a synergistic combination of effective parameters interactions and these parameters were in equilibrium, according to a modeling assessment of strain AFNA and its correlation with bacterial growth.

48 citations


Journal ArticleDOI
TL;DR: In this paper, the authors determined the methanogenic toxicity effect of free ammonia contained in swine wastewater comparing raw swine waste (RW) and the liquid fraction of SWW (TW).

43 citations



Journal ArticleDOI
TL;DR: Phylogenetic analysis of the mixed culture revealed that members involved hydrogen producers in both batch and continuous reactors were phylogenetically related to the Thermoanaerobacterium thermosaccharolyticum .

41 citations


Journal ArticleDOI
TL;DR: Significant QTLs detected in this study can be employed in MAS for molecular breeding programs aiming at developing drought tolerant cotton cultivars through molecular marker-assisted breeding.

Journal ArticleDOI
TL;DR: The results suggest that the high degree of EST-SSRs transferability to Linum species can be a useful enhancement of the current database of SSR markers for future genetic and evolutionary studies.

Journal ArticleDOI
TL;DR: The saccharification of lignocellulosic substrates by enzyme produced by A. oryzae ITCC 4857.01 indicates the enzymes specificity towards the substrates and will lead to efficient conversion of cellulose materials to other important products.

Journal ArticleDOI
TL;DR: In this article, the authors used DSY as a low-cost nitrogen supplement for ethanol fermentation from sweet sorghum juice under very high gravity (VHG) conditions by Saccharomyces cerevisiae NP 01.

Journal ArticleDOI
TL;DR: An automated colony picker, Clone Pix FL system was used to replace limiting dilution method and was able to screen 1 x 10 5 clones secreting humanized monoclonal antibodies and high producer clones were selected in just 7 days.

Journal ArticleDOI
TL;DR: The effects of fermentation temperatures and yeast strain type on beer quality and spent yeast density produced after wort fermentation by two commercial yeast strains were investigated in this paper, and the results showed that there was no significant difference in colour intensity between the beers produced at different fermentation temperatures.

Journal ArticleDOI
TL;DR: In this paper, the efficiency of sugar cane bagasse (SCB) and vegetable sponge (VS), two natural adsorbents commonly found in Brazil, for retaining paracetamol molecules dispersed in aqueous solutions was evaluated.

Journal ArticleDOI
TL;DR: In this article, the effect of pH and temperature on dye uptake was also investigated and kinetic parameters were determined, including optimal initial pH (3.0), equilibrium time (2 hrs), initial dye concentration ( 100 mg l -1 ) and biomass concentration (1.2 mg l −1 ) were determined at 26oC.

Journal ArticleDOI
TL;DR: In this article, the aerobic degradability of nonylphenol ethoxylates (NPEOs) by the acclimated microorganisms in active sludge was examined using shaking-flask tests, and the degradation of benzene rings in NPEOs was determined using UV spectroscopy and high performance liquid chromatography (HPLC).

Journal ArticleDOI
TL;DR: In this paper, the effect of feeding rate (0.10-0.15 L h -1 ) on 1,3-propanediol production was studied in fed-batch fermentation, where the optimal condition was 60 g l -1 crude glycerol, pH control at 6.5 and cultivation temperature at 37oC.

Journal ArticleDOI
TL;DR: AFLP is an efficient tool for determination of genetic diversity of cabbage gene bank accessions and ten selective primer pairs were found to be most informative because each of these uniquely identified all of the accessions used.

Journal ArticleDOI
TL;DR: Investigation of the knowledge and acceptance of genetically modified organisms among prospective teachers in Slovenia and Turkey revealed that knowledge of genetics and biotechnology barely influenced the acceptability of genetic modification, and correlations are low.

Journal ArticleDOI
TL;DR: It has been found that the best condition for CLEAs preparation are to use glutaraldehyde as cross-linking reagent at pH 9.5, at a concentration of 3.5 g/l, and an enzyme/albumin ratio of 15.2%.

Journal ArticleDOI
TL;DR: In this paper, a low-cost media for the production of LAB plantarum biomass was proposed, where distillery waste is combined with a byproduct from a sugar production, molasses, to form a medium for the growth of Lactobacillus plantarrum with yields and biomass densities comparable with conventional industrial media.

Journal ArticleDOI
TL;DR: In this article, an inertizing and cooling process for grapes cryomaceration has been set up, which works with a grape flow of approximately 2-3 tons/h, with a maximum thermal gradient of 20 K between the grape inlet and outlet temperature.

Journal ArticleDOI
TL;DR: The filamentous fungus A. phoenicis produced high levels of β-D-fructofuranosidase (FFase) when grown for 72 hrs under Solid-State Fermentation (SSF), using soy bran moistened with tap water (1:0.5 w/v) as substrate/carbon source.

Journal ArticleDOI
TL;DR: The objective of this study was to investigate the effect of vermicompost, worm-bed leachate (WBL) and Glomus mosseae, an arbuscular mycorrhizal fungi (AMF) on growth of lemongrass and its composition.

Journal ArticleDOI
TL;DR: The objectives of this study were to investigate whether 17β-estradiol could be measured from individual cow milk samples using a commercially available non-radiolabelled enzyme immunoassay kit (EIA) with no previously reported milk application, and whether those detections could precisely illustrate 17 β-mostradiol pre-ovulation peak.

Journal ArticleDOI
TL;DR: It is concluded that the use of adaptive digital learning material is a possible way to solve the problem of differences in prior education of students entering a course.

Journal ArticleDOI
TL;DR: The data suggest the possibility of using a medium of non-animal origin and employing pH control for the cultivation of pneumococcus to produce a polysaccharide vaccine.