Showing papers in "Experimental Parasitology in 2013"

TL;DR: A quantitative qPCR assay using minor groove binder-probes targeting a unique Cryptosporidium specific protein-coding gene, that directly detects, quantitates and identifies C. hominis and C. parvum in environmental and faecal samples is developed and validated.

TL;DR: The toxicity of mosquito larvicidal activity of leaf essential oil and their major chemical constituents from Ocimum basilicum were evaluated against Culex tritaeniorhynchus, Aedes albopictus and Anopheles subpictus and the results could be useful in search for newer, safer, and more effective natural larvicide agents.

TL;DR: In the in vitro assays and in vivo studies, the SEM observations revealed structural alterations in the worms after contact with TR plants when compared to the control worms, which concerned the cuticle and the buccal area.

TL;DR: A novel multiplex PCR for Theileria annulata, Babesia bovis and Anaplasma marginale which has been validated using field samples and can be applied to epidemiological studies.

TL;DR: The dry format UCP-LF assay was shown to provide a robust and easy to use format for rapid testing of CAA antigen in serum and was found to be superior with respect to speed and simplicity of use.

TL;DR: Ethnopharmacological data indicate Annona muricata as a promising alternative for the control of gastrointestinal nematodes because of its general anthelmintic properties and validate the traditional use of A. muricATA as a natural anthel Mint as well as the pharmacological potential of its compounds for future in vivo investigations.

TL;DR: The results showed that the recombinant TspSP-1.2 protein induced a partial protective immunity in mice and could be considered as a potential vaccine candidate against T. spiralis infection.

TL;DR: The aim of this work was to assess the monoamine systems activity in specified brain regions involved in the natural defense behaviors, emotion evaluation, and motor and sensory stimuli integration in experimentally T. gondii infected mice compared to uninfected controls and to discuss the results in terms of their possible contribution to T. Gondii-induced intermediate host behavior alterations and parasite transmission.

TL;DR: The results show that RLNs are clearly amenable to gene silencing, and that in planta delivery of dsRNA to target genes in these nematodes should confer host resistance.

TL;DR: It was determined that non-visible light-exposed TiAg-Nps were more effective against L. tropica and L. infantum parasites while visible light-Exposed Ti Ag-NPS exhibited nearly the same antileishmanial effect against both species.

TL;DR: Results indicate that miltefosine induced apoptosis like death in a time and dose dependent manner in wild-type cells, but not in MIL-resistant cell line, and hypothesize that oxidative stress mediated apoptosis as an alternative mechanism of milteFosine resistance.

TL;DR: The finding of the same two dominant IIa and IId C. parvum subtypes recently found in humans in Egypt suggests calves can be potential reservoirs of zoonotic cryptosporidiosis.

TL;DR: The nano-drug AMB delivery system appeared more effective than free D-AMB therapy to reduce the dose frequency required to achieve the same therapeutic level, and thus favors a longer interval between doses, as expected with development of a new nano drug delivery system, and may be useful in the treatment of many different pathologies.

TL;DR: Real-time PCR, conventional PCR, and the direct agglutination test (DAT) were used to diagnose Leishmania infantum infection in blood samples from 100 domestic dogs and 100 humans to diagnose Zoonotic visceral leishmaniasis in northwestern Iran.

TL;DR: The similar efficacy of EncEs and EsEO demonstrates that there was no optimization of anthelmintic action following the encapsulation process, and the use of new encapsulation matrices with controlled release in the pH of the abomasum should be investigated.

TL;DR: The findings support the idea that distinct biomolecules might be involved in antimony resistance in L. tropica field isolates and are the first report of identification of PTP and PGK genes potentially implicated in resistance to antimonials.

TL;DR: A protocol for interlaboratory comparison of conventional and real-time PCR methods involving four participant laboratories from four different endemic regions in four continents is proposed; the protocol includes a quality control step and reduces the variability among the samples tested by each participant.

TL;DR: The results suggested that chicken IL-17 might mediate E. tenella - induced immunopathology during the infection, and resulted in reduced fecal oocyst shedding and cecal lesion scores, but enhanced body weight gains were observed in IL- 17 neutralized chickens.

TL;DR: Findings indicate, for the first time, that oils of andiroba and aroeira (in their conventional and nanoemulsion forms) have high activity against T. evansi in vitro, leading to the suggestion that these oils may be applied as an alternative treatment for this disease.

TL;DR: The parasitic castration in the B. glabrata-S.

TL;DR: The higher microsatellite diversity found in P. vivax isolates, compared to sympatric P. falciparum isolate, does not necessarily result from local differences in transmission level and may reflect differences in population history between species or increased mutation rates in P Vivax.

TL;DR: The leishmanicidal activity demonstrated by the coumarin and acetogenins indicate these compounds for further studies aiming the development of new leish manicidal agents.

TL;DR: The LAMP-Tube scanner method is found to be simple and allowed real-time detection of DNA amplification and is an alternative to conventional PCR-based diagnostic methods for field use.

TL;DR: This is the first description of new Hepatozoon species from snakes, based on molecular characterization and morphological data, in South America, and based on the molecular and morphologic differences, three new species were proposed.

TL;DR: Data is presented on sporogonic development of 2 widespread mitochondrial cytochrome b lineages (cyt b) of P. relictum (pSGS1 and pGRW11) in mosquito Culex pipiens pipiens indicating that phylogenetic trees based on the cyt b gene likely can be used for predicting sporogony development of genetically similar avian malaria lineages in mosquito vectors.

TL;DR: Although the cysts showed reduced size and weight in the treated animals but these reductions were not statistically significant, these ultrastructural changes should be supported by further biochemical and molecular studies before introducing it as an efficient therapeutic regimen for treatment of human and animal hydatid disease.

TL;DR: It is demonstrated that the PI3K-PKB/Akt pathway may be one of the major route for T. gondii in the prevention of host cell apoptosis and T.gondii phosphorylates the pro-apoptotic Bad protein to prevent apoptosis.

TL;DR: The ability of antimicrobial peptides derived from human cathelicidin to damage Entamoeba trophozoites is demonstrated, and it was shown that the integrity of the peptides is altered in the presence of an ameba soluble fraction with cysteine protease activity.

TL;DR: These results demonstrate the existence of complex patterns of shared larval glycan constitutively expressed on hemocytes and their proteins, as well as the ability of hemocytes to acquire shared glycans by the selective binding of parasite-released LTP.

TL;DR: The results suggest that Ch TLR4 and ChTLR15 are involved in response to E. tenella infection, and may operate in a MyD88-dependent manner for host defense.