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Showing papers in "Journal of Bioscience and Bioengineering in 2013"


Journal ArticleDOI
TL;DR: Application of biochar in soil has been shown to result in decreased efficacy of pesticides, which indicates a trade-off between the potentially promising effect of bio char on pesticide remediation and its negative effect on pesticide efficacy.

455 citations


Journal ArticleDOI
TL;DR: Recent applications of metabolomics in microbiology, plant, animal, food, and medical science are discussed and integrated applications with genomics, transcriptomics, and proteomics provide greater understanding of global system biology.

216 citations


Journal ArticleDOI
TL;DR: The latest development of analytical techniques and data analyses methods in metabolomics study are discussed and the most meaningful elements of the data are extracted by using various data analysis tool.

167 citations



Journal ArticleDOI
TL;DR: The history of SFC was of "the rise and fall," advances in chiral stationary phase took place in the early 1990s made packed-column SFC truly useful chiral separation method and SFC is now regarded as an inevitable separation tool both in analytical and preparative separation.

142 citations


Journal ArticleDOI
TL;DR: It was found that lactic fermentation reduced the content of saponins and phytates and enhanced the total phenolic content as well as antitumor cell proliferation effect of soymilk against HT-29 and Caco-2 cells.

135 citations


Journal ArticleDOI
TL;DR: This work presents a rapid transformation technique for cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator, which could be applied to transformation of other industrially useful algae by optimizing the electric conditions.

122 citations


Journal ArticleDOI
TL;DR: Results indicate that curcumin acts to reduce cisplatin-induced nephrotoxicity through its anti-inflammatory effects and may become a new therapeutic candidate for the treatment of cisPlatin- induced neph rotoxicity.

121 citations


Journal ArticleDOI
TL;DR: Stability and stabilization methodologies of enzymes in scCO(2) as well as reactions for organic synthesis are described and varieties of examples for lipase-catalyzed synthesis of chiral compounds using scCO (2) are given.

80 citations


Journal ArticleDOI
TL;DR: Recent advances in the field regarding PLD-catalyzed synthesis of bioactive PLs, deeper understanding of substrate recognition and binding mechanism, altering substrate specificity, and improving thermostability are focused on.

71 citations


Journal ArticleDOI
TL;DR: To develop a microbial production platform based on hydrogen and carbon dioxide, a genetic transformation system for the thermophilic acetogen Moorella thermoacetica ATCC39073 was developed and reintroduction of native pyrF into the mutant by homologous recombination ensured recovery from uracil auxotrophy.

Journal ArticleDOI
TL;DR: It is demonstrated that the light/electricity conversion occurs via syntrophic interactions between phototrophs and electricity-generating bacteria, and it is estimated that the excretion of organics by thePhototroph was the bottleneck step in the syntrophic light/Electricity conversion.

Journal ArticleDOI
TL;DR: Practical PUFA production through mutant breeding, functional analyses of the genes of the enzymes involved in PUFA biosynthesis, and recent advances in unique PUFAs production through molecular breeding are described.

Journal ArticleDOI
TL;DR: The coding region of the ccpAx gene (ccp gene from Acetobacter xylinum) is identified and the localization of the CcpAx in cells by generating fusion proteins tagged to an enhanced green fluorescent protein (EGFP).

Journal ArticleDOI
TL;DR: In this paper, a stepwise genome reduction approach was used to create an Escherichia coli strain with a smaller genome, which has a genome of 3.62 megabase pairs (Mbp).

Journal ArticleDOI
TL;DR: The presented heterogeneous hydrogel sheet could be useful, as it provides relatively large, but precisely-controlled, 3-dimensional microenvironments for the high-density coculture of multiple types of cells.

Journal ArticleDOI
TL;DR: Electrolysis of water in alginate solutions with calcium carbonate particles induced gel aggregation around Pt wire electrodes, forming tubularAlginate gel structures, which are a promising approach for construction of multi-layer tubular hydrogel structures for tissue engineering.

Journal ArticleDOI
TL;DR: Two L-asparaginase homologs have been found in the genome of Thermococcus kodakaraensis and unfolding studies suggested that urea could not induce complete unfolding and inactivation of TK1656 even at a concentration 8 M; however, in the presence of 4 M guanidine hydrochloride enzyme structure was unfolded with complete loss of enzyme activity.

Journal ArticleDOI
TL;DR: The utility of CE-MFC for wastewater treatment, in which Geobacter and Geothrix would be involved in the electricity generation, is demonstrated.

Journal ArticleDOI
TL;DR: Thin stillage (TS), wastewater from rice wine distillery, was used as a cost-free feedstock to replace the costly traditional Hestrin and Schramm (HS) medium for BC production by Gluconacetobacter xylinus.

Journal ArticleDOI
TL;DR: Results showed that cell-based patterns in a 3D matrix are highly dependent on both cell aggregation and migration over time.

Journal ArticleDOI
TL;DR: The gene encoding a β-glucosidase from the archaeon Thermofilum pendens was cloned and expressed in Escherichia coli and showed great potential in cellulose saccharification and the glucoside hydrolysis of natural compounds.

Journal ArticleDOI
TL;DR: It is shown that the cooperativeness was kept intact by at least two residues, Glu89 and His465, of which Glu 89 is newly identified to be involved in the cooperativity system of GAD, which might be utilized to convert a low-valued by-product of biodiesel production into value-added product, GABA.

Journal ArticleDOI
TL;DR: Twenty bacterial strains were examined on their ability to produce polyhydroxyalkanoates (PHA) from different carbon sources under rich and depleted nitrogen conditions and an atypical metabolic shift was observed.

Journal ArticleDOI
TL;DR: Improved of fermentation aeration and the application of fed-batch system significantly enhanced 1,3-butanediol production and the selection of heterologous genes from several organisms and activity level of their corresponding gene products were demonstrated to be the key element for the product formation.

Journal ArticleDOI
TL;DR: In this study, a series of CD derivatives were designed and synthesized to improve the QS inhibitory activity over that of native CDs, and the synthetic alkylamine-modified CD derivatives had higher equilibrium binding constants for binding with AHL than the native CDs did, consistent with the improved QS inhibition.

Journal ArticleDOI
TL;DR: This investigation was aimed at detoxifying the toxic and anti-nutritional compounds in J. curcas seed cake by fermentation with Bacillus spp.

Journal ArticleDOI
TL;DR: The present work is the first part in the direction of enzymatic membrane reactor studies for carrying out reaction followed by membrane based separation to purify galacto-oligosaccharides out of reaction mixture.

Journal ArticleDOI
TL;DR: The obtained results confirmed that nitrogen limitation is unnecessary for the stimulation of biopolymer synthesis and confirmed that the extracted mcl-PHAs are elastomers with useful physical and chemical properties.

Journal ArticleDOI
TL;DR: A large number of microorganisms are considered to interact and cooperate with each other in various natural environments, such as dropped cereal crops or ripe fruits, and human beings have taken advantage of these microbial interactions for producing various fermented foods.