Showing papers in "Journal of Immunological Methods in 1988"

TL;DR: This LDH release assay combines the advantages of reliability and simple evaluation characteristic of radioisotope release assays with the convenience of speed and avoidance of radioactivity and suggests that LDH releases are an appropriate and possibly preferable means of measuring cellular cytotoxic reactions.

TL;DR: A reverse modification of ELISPOT assay using nitrocellulose membranes and epitope-specific monoclonal antibodies is described for the detection of single lymphokine-secreting cells.

TL;DR: It is demonstrated that mitotic cells had a very low PCNA signal and monoclonal PCNA-specific antibodies offer a convenient tool for the detection of human cell proliferation by immunofluorescence microscopy and by flow cytometry.

TL;DR: A variation of the standard ELISA assay was used to determine the relative affinities of six murine monoclonal anti-dinitrophenol (D NP) antibodies for DNP-bovine serum albumin (DNP-BSA), demonstrating the applicability of the elution technique for the measurement of relative antibody affinity.

TL;DR: Hundreds of hybridomas secreting antibodies that bound to phosphotyrosine were detected by ELISA and should be useful for the identification and purification of proteins phosphorylated on tyrosine residues in transformed and growth factor-treated cells.

TL;DR: It is found that the intrinsic forward reaction rate in the bimolecular antigen-antibody reaction is normally not limited by diffusion either in solution or at the solid-liquid interface, however, reactions at theSolid- liquid interface can be diffusion limited due to depletion of reactants close to the surface.

TL;DR: A dual wavelength spectrophotometric assay that permitted beta-lactamase and beta-galactosidase activities to be measured concurrently in a single sample was developed and examples of its use to monitor and dissect the action of biological agents on a gram-negative bacterium are provided.

TL;DR: A novel assay system has been developed which is based on the ELISPOT methodology and employs a combination of two immunoenzyme visualization systems yielding distinct colour products that permits the simultaneous enumeration of two different types of cell secreting antigenically distinct products.

TL;DR: Bromodeoxyuridine is used in place of thymidine and measurement of incorporation of this molecule is performed by an immunoenzymatic assay (using monoclonal anti-BrdUrd), which avoids the expense, time and hazards associated with scintillation counting, and is simpler to perform.

TL;DR: Observations suggest that the two types of PI-PLC may have distinct substrate specificities or sensitivities to environmental conditions which account for the difference in their ability to act on PI-anchored proteins in particular cell types.

TL;DR: Using human CTL and natural killer cells as effectors, with a variety of lymphoid cells and fibroblasts as targets in 4 h assays, the BCECF retention technique was found to give cytotoxicity values comparable to the 51Cr release assay.

TL;DR: A method to measure the relative affinity of antibodies using an inhibition enzyme immunoassay is described and is validated using monoclonal antibodies of defined affinity characteristics and by comparison with conventional methods of affinity measurement.

TL;DR: In an attempt to improve the diagnostic value of measuring antibodies to islet cell cytoplasmic antigen, coded sera were distributed to 38 laboratories and results were returned for analysis and a substantial improvement was obtained.

TL;DR: A new method for immobilization of antibodies to solid supports by covalent bonds between aldehydes generated on the carbohydrate side chains of the antibody and hydrazide groups on the solid support is described.

TL;DR: The construction of a new heteromyeloma cell line derived from xenogeneic somatic cell hybridization between normal human B lymphocytes and the murine HAT-sensitive P3X63Ag8/653 cell line and the generation of hybrids secreting human monoclonal antibodies to human immunodeficiency virus is described.

TL;DR: Specific antisera and calcium-independent antibodies were used to develop and characterise solution and solid-phase immunoassays specific for free trypsinogen activation peptides (TAP assay), which may contribute to the recognition of disease states such as pancreatitis.

TL;DR: A simple and efficient non-chromatographic method for the purification of murine IgG3 and IgM monoclonal antibodies (MAbs) which takes advantage of their euglobulin properties, and the purified immunoglobulin was essentially free of albumin, transferrin, and other mouse ascites proteins.

TL;DR: IgG4 antibodies to TT are of lower functional affinity than IgG1 antibodies and that, in addition, IgG4 responses to TT display a more restricted affinity heterogeneity.

TL;DR: An ELISA for determining and quantifying ACPA using an affinity-purified antigen preparation that provides precise ACPA quantitation and should prove valuable for monitoring disease activity in WG.

TL;DR: To determine, whether a sporozoite is outside the hepatocyte membrane or internalized, a double staining test was carried out using, successively, antibody labeled with peroxidase and fluorescein.

TL;DR: With these methods it is now possible to characterize the phenotype of dividing cell populations such as precursors in central lymphoid tissues and germinal centre blasts in peripheral lymphoid organs.

TL;DR: Chicken antibodies should therefore be useful in assays were RF could interfere and give false positive reactions (e.g., nephelometry, latex agglutination or ELISA).

TL;DR: A two-step chromatographic procedure was developed for the isolation and purification of hen IgY antibodies from egg yolk as mentioned in this paper, where the antibodies were completely separated from vitellin and lipids by hydrophobic interaction chromatography followed by gel filtration.

TL;DR: The histamine content of the cell pellets was found to be strongly correlated with the mast cell count and was estimated to about 10 pg/cell, which is higher than previously reported for mast cells obtained from human lung tissue dispersed by an enzymatic method.

TL;DR: The discontinuous system (MPRM-HP) was capable of resolving leukocyte fractions from blood volumes as small as 1 ml with excellent purity and yield and was compatible with a wider range of anticoagulants and permitted fractionation of specimens resistant to MPRM.

TL;DR: A microassay for the detection of cellular adherence to monolayers of cultured human vascular endothelium has been developed that utilises the uptake by cells of the vital stain, Rose Bengal, with special reference to neutrophils using a range of stimulators that may regulate their attachment to endothelia.

TL;DR: Using these methods an antigen-specific secretory IgA anti-cholera toxin B sub unit response in the secretions of volunteers given an oral B subunit vaccine was readily demonstrated.

TL;DR: Four sensitive sandwhich-enzyme-linked immunosorbent assays (ELISAs) have been developed for the detection of native C3 or C5 in samples with low concentrations of these proteins, e.g., in cell culture supernatants or synovial fluids and cerebrospinal fluids (CSF).

TL;DR: The results show that some peptides needed stringent pH conditions while others could be coated in a broad pH range, and the addition of 0.6 M NaCl had a favorable effect on peptide coating.

TL;DR: This is the first report of a single, well-defined T cell product measurement by the spot-ELISA, a highly sensitive, easy to perform and rapid assay suitable to detect production of other lymphokines at the single-cell level.