Showing papers in "Journal of Lipid Research in 1972"

TL;DR: Gel chromatography on 2% agarose has been shown to be an effective quantitative method for separating triglyceride-rich lipoproteins according to particle size and results obtained in subjects with dysbetalipoproteinemia were consistent with the presence of three populations of particles.

TL;DR: Thin-layer chromatography revealed important differences among the plasmas from the three species, but all possessed hematosides and hexosamine-containing gangliosides.

TL;DR: The bulk of the evidence suggests that, in all forms of hyperlipidemia except fat-induced hyperglyceridemia, the drug causes an increased output of cholesterol while simultaneously inhibiting any compensatory increase in cholesterol synthesis.

TL;DR: A classification for genetic obesity based primarily upon the cellularity characteristics of the adipose depots is proposed.

TL;DR: The possibility that triglyceride lipase in postheparin plasma may be heterogeneous and that levels of the enzyme in post heparin Plasma may not accurately reflect the capacity for clearance of triglyceride from the plasma is raised.

TL;DR: Experimental data are presented on the intracellular localization in rat liver of three enzymes which are involved in the biosynthesis of phosphatidylethanolamine and diphosphatidylglycerol, finding that the first two enzymes are primarily mitochondrial while the latter enzyme is primarily microsomal.

TL;DR: Results obtained in this study support the hypothesis that the major portion of the rise in HMG CoA reductase activity and the increase in overall rate of cholesterol synthesis in liver and intestine during the circadian rhythm are due to the ingestion of food.

TL;DR: The galactose oxidase-sodium borohydride method was used to specifically label the terminal N-acetylgalactosamine of three glycosphingolipids, Gm2-ganglioside, asialo-Gm2 -ganglioide, and globoside, which were well suited as substrates for the study of specific sphingolIPid N- acetylgalactsosaminidase.

TL;DR: The mean cell sizes of specimens of human adipose tissue were determined on sectioned slices according to the method described by Sjöström et al. and on adipocytes isolated after treatment of the tissue with collagenase and there was no indication of an increased rupture of isolated largehuman adipose cells.

TL;DR: The incorporations are inhibited by a variety of structurally related alcohols and are stimulated by isoserine (alpha-hydroxy,beta-aminopropionic acid), which appears to be energy-independent since Mg(2+), CTP, ATP, and NaF have no stimulatory action.

TL;DR: The enzymatic hydrolysis in vitro of the esters of methanol, ethylene glycol, glycerol, erythritol, pentaerythredol, adonitol, sorbitol, and sucrose in which all alcohol groups were esterified with oleic acid was studied.

TL;DR: The results suggest that the effects of PGE(2) in clearing the scales may be associated with its inhibitory effect on abnormal sterol esterification in the skin of the EFA-deficient rats.

TL;DR: The microsomal HMG CoA reductases of liver and intestinal mucosa had similar kinetic characteristics and pH optima, however, the activity of the hepatic enzyme differed with age and sex of the experimental animals while that of the intestinal crypt cells did not.

TL;DR: It was found that adipose tissue from areas where fat is deposited very readily (particularly the perirenal region) had higher enzyme activities, larger adipose cells, a lesser amount of stromal tissue, a greater amount of ether-extractable lipid, and a lower concentration of adipose Cells per gram of tissue.

TL;DR: Cholesterol accumulation produced by ethanol feeding is associated with both enhanced cholesterogenesis and decreased bile acid excretion, and the latter is probably predominant in these studies in which cholesterol accumulation was markedly enhanced by the addition of cholesterol to the ethanol-containing diet.

TL;DR: The recombination procedure was employed to examine the specificity of the binding of retinol to RBP, by determining whether compounds other than all-trans-retinol would effectively bind to apo-RBP.

TL;DR: The mechanism of control of plasma cholesterol levels is related to the rate of conversion of cholesterol to bile acids, and it is concluded that, in this species, hyporesponders responded more slowly and to a lesser degree.

TL;DR: The results demonstrate that careful use of the described method yields accurate measurement of unidirectional uptake rates of lipids across the brush border that are of critical importance in defining the characteristics of membrane penetration and the rate-limiting steps in fat and sterol absorption.

TL;DR: The results imply that the previously reported diminished responsiveness to insulin shown by large adipose cells is exerted only on the side of lipid accumulation, and it is suggested that the negative correlation between cell size and responsiveness to glucose in the medium may be one way to control adipose cell enlargement.

TL;DR: The results suggest that the rate-limiting and principal regulatory step in the biosynthesis of eicosa-5,8,11-trienoic acid is the 6-desaturation of oleic acid.

TL;DR: Hydrocarbons from three homologous series of branched alkanes from the eggs of the tobacco hornworm, Manduca sexta (L.), were identified by mass spectrometry.

TL;DR: A diet containing 20% corn oil was a much weaker inducer of the enzyme but caused a prolonged elevation that began late in the fasting part of the cycle, suggesting two different mechanisms of action.

TL;DR: Gel electrophoretic and immunochemical evidence indicates that a peptide appears in lipoproteins from cholesterol-fed, anemic guinea pigs that is undetectable in those of controls, similar to that reported in patients with obstructive jaundice, biliary cirrhosis, type III hyperlipoproteinemia, or familial lecithin:cholesterol acyltransferase deficiency.

TL;DR: An apparatus and procedure are described for investigating fat transport and lipoprotein biosynthesis in isolated, lymph-cannulated rat intestine perfused with blood under physiological conditions, indicating that d 1.006-1.006 lipoproteins are produced by gut and reach the blood via mesenteric lymph.

TL;DR: These fractions represent two species of lipoprotein lipase, each with a requirement for serum for substrate activation, inhibition by 1 m NaCl, and an alkaline pH optimum (pH 8.0).

TL;DR: In this review, emphasis has been placed on the in vitro studies with cell-free systems, since these represent the groundwork for further purification and characterization of the enzyme systems involved.

TL;DR: In the bile fistula rats, somewhat more of the lipoprotein cholesterol than of the particulate cholesterol appeared in bile early after injection, however, cholesterol turnover calculated from a twopool model was the same for rats injected with lipop Proteinbound or particulates cholesterol.

TL;DR: Carotene 15,15'-dioxygenase, which oxidizes carotenoids to retinal, has been purified up to 200-fold from rabbit intestine by ammonium sulfate fractionation, heat treatment, and acetone precipitation.

TL;DR: Chemical and enzymic evidence suggests the use of the term "lactosylceramidosis" for this disease.

TL;DR: Metabolic indices-levels of circulating free fatty acids, glycerol, and in some cases glucose and lipid-determined at various ages in fed "fatties" and controls, and at intervals during prolonged fasting, were consistent with a picture of excessive adipose tissue lipolysis, excessive reesterification in the adipose tissues, fat mobilization in excess of need, and return of the excess to the adiposes tissue via lipoproteins.