Showing papers in "Journal of Lipid Research in 2022"

TL;DR: The field of lipid metabolism was recognized but difficult to mechanistically understand due to the lack of sensitive and robust technologies for identification and quantification of lipid molecular species as mentioned in this paper , and the enabling technological breakthroughs emerged in the 1980s with the development of soft ionization methods (Electrospray Ionization and Matrix Assisted Laser Desorption/Ionization) that could identify and quantify intact individual lipid species.

TL;DR: In this paper , the effect of the SGLT2 inhibitor dapagliflozin (1 mg/kg/day, formulated in a water, PEG400, ethanol, propylene glycol solution, 4 weeks) on lipid metabolism in obese Zucker rats was examined.

TL;DR: In this article , the authors conducted LC/MS/MS analyses to detect individual ceramide species differing in both the LCB and FA chain lengths and quantified 1,327 unbound and 254 protein-bound ceramides.

TL;DR: In this paper , the authors discuss recent advancements in tandem mass spectrometry (MS/MS) methods for identification of complex lipids beyond the species (known headgroup information) and molecular species(known chain composition) levels.

TL;DR: The lipid envelope of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an essential component of the virus; however, its molecular composition is undetermined as discussed by the authors .

TL;DR: The lipid envelope of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an essential component of the virus; however, its molecular composition is undetermined as mentioned in this paper .

TL;DR: In this article , the impact of truncated perilipin 5 (PLIN5) in comparison to disrupted LDMC on cellular TG homeostasis, FA oxidation, mitochondrial respiration, and protein interaction was investigated.

TL;DR: In this paper , differences between the Friedewald, Martin-Hopkins, and NIH equation 2 methods of calculating LDL-C and the "gold standard" BQ method using pooled phase 3 data with alirocumab were assessed.

TL;DR: Oxysterols have interested chemists, biologists, and physicians for many decades, but their exact biological relevance in vivo, other than as intermediates in bile acid biosynthesis, has long been debated as mentioned in this paper .

TL;DR: In this article , an anti-ANGPTL3/8 antibody targeting the same leucine zipper-containing epitope recognized by LPL and ApoA5 was developed.

TL;DR: In this paper , the 3-ketosphinganine reductase activity of several candidate proteins in Bacteroides thetaiotaomicron was analyzed based on sequence similarity to the yeast 3-kkRNAs.

TL;DR: In this article , the role of neutral ceramidase (nCDase) in the development of acute kidney injury (AKI) was further characterized by demonstrating that nCDase−/− mice are resistant to cisplatin-induced AKI.

TL;DR: In this paper , the authors used a genetic dissection approach in mice to examine the contributions of these proteins in select tissues, and found that increased storage of retinoids in peripheral tissues of ISX-deficient mice was dependent on STRA6 and induced by retinoid signaling.

TL;DR: A review of the role sortilin plays in cardiovascular and metabolic diseases and describe in detail the large and often contradictory literature on the role of Sortilin in the regulation of LDL-C levels is presented in this paper .

TL;DR: In this article , the role of excessive GlcSph formation during glucocerebrosidase deficiency was studied in zebrafish that have two orthologs of acid ceramidase, Asah1a and Asah 1b.

TL;DR: In this article , the authors identified transgelin (TAGLN), an actin-binding protein, as a potentially new gene involved in LDL endocytosis and showed that TAGLN-KO cells exhibited a reduction in total and free cholesterol content, activation of SREBP2, and a compensatory increase in cholesterol biosynthesis.

TL;DR: In this paper , the authors used cold exposure as an acute stress, and performed global lipidomics on plasma and in nine tissues that may contribute to the circulating lipid pool and found that numerous species of plasma acylcarnitines and ceramides were significantly altered upon cold exposure.

TL;DR: In this paper , 1-deoxysphinganine (dSA) was identified as a proximal mediator of SG deprivation in SK1 loss and Sph level elevation upon SG deprivation.

TL;DR: In this article , the ability of VLDL from 15 normolipidemic donors to act as substrates for human LPL was tested using a novel assay designed to mimic human plasma conditions in vitro, and they found a 10-fold difference in hydrolysis rates across individuals when the particles were compared on a protein or a TG basis.

TL;DR: In this article , the role of linoleate moiety in ω-O-acylceramides in the assembly of the skin lipid barrier was explored, and it was shown that linoleic acid esterified to the terminal hydroxyl of the Nacyl, the formation of which requires the transacylase activity of patatin-like phospholipase domain containing 1 (PNPLA1).

TL;DR: In this article , a systematic review of available evidence on the natural history and biomarkers of LCAT deficiency, in order to guide the development of novel therapeutics is presented, which provides the first step toward the identification of disease biomarkers to be used in clinical trials and suggests that restoring LCAT activity to subnormal levels may be sufficient to prevent renal disease progression.

TL;DR: In this paper , the authors compared all-atom molecular dynamics simulations on bovine AAC1 in lipid bilayers with and without CLs and found that CL binding does not significantly affect overall stability of the carrier or structural symmetry at the matrix-gate level.

TL;DR: In this paper , a new isoform independent enzyme-linked immunoassay (ELISA) was developed for the measurement of lipoprotein (a) [Lp(a)], validate its performance characteristics, and demonstrate its accuracy by comparison with the gold-standard ELISA method and an LC-MS/MS candidate reference method, both developed at the University of Washington.

TL;DR: In this article , the authors investigated the role of lipid asymmetry in the formation of ordered lipid domains in a rat leukemia RBL-2H3 cell. And they concluded that loss of PMV lipid asymmetric can induce ordered domain formation.

TL;DR: In this article , the authors used patient-derived fibroblast cells as a surrogate tissue for metabolic and lipidomic profiling by liquid chromatography-high resolution mass spectrometry.

TL;DR: In this article , the authors investigated the metabolic partitioning of cellular extracellular FAs and found that labeled FAs from uptake were largely incorporated to canonical (sn-) positions on the glycerol backbone.

TL;DR: In this paper , the authors showed that decreasing C1P activity via inhibitors or genetic ablation of the anabolic enzyme ceramide kinase (CERK) significantly enhanced wound healing phenotypes, and postwounding inhibition of CERK enhanced the closure rate of acute wounds, improved the quality of healing, and increased fibroblast migration via a class switch.

TL;DR: In this paper , the authors analyzed human serum with a commonly applied high-resolution LC-MS untargeted metabolomics platform and found that many chemical features are putatively assigned to the FAHFA lipid class based on exact mass and fragmentation patterns matching spectral databases.

TL;DR: In this paper , the authors explored the signaling crosstalk between cholesterol and the actin cytoskeleton by using a high-resolution confocal microscopic approach to quantitatively measure changes in F-actin content upon cholesterol depletion.

TL;DR: In this paper , the authors used Cox proportional regression analysis and a case-cohort design to test associations of HDL proteins with incident CVD (myocardial infarction, coronary artery bypass grafting, angioplasty, or death from coronary heart disease).