Showing papers in "Journal of Steroid Biochemistry in 1988"

TL;DR: In MCF-7 and ZR-75-1 breast cancer cells in tissue culture ICI 164384 was a more potent inhibitor of cell growth than tamoxifen and growth inhibition was reversed by oestradiol.

TL;DR: In vivo studies demonstrated that injection of luteolin or quercetin blocked estradiol stimulation of nuclear type II sites in the immature rat uterus and this correlated with an inhibition of uterine growth (wet and dry weight).

TL;DR: The athymic mouse model can be used in the future to determine the efficacy of novel antiestrogens and the development of antiestrogen drug resistance and to support the view that therapy must be given indefinitely to patients to control tumor recurrence.

TL;DR: The immunosuppressive actions of glucocorticoids form the basis for their use in treatment of autoimmune diseases and prevention of allograft rejection and, in some cases, may enhance certain functions of immune effector cells.

TL;DR: It is demonstrated that ICI 164,384 has many of the characteristics of a pure antioestrogen.

TL;DR: FCE 24304 (6-methylenandrosta-1,4-diene-3,17-dione), a new irreversible aromatase inhibitor, has been identified and characterized in vitro and in vivo and little binding affinity for the androgen receptor was observed.

TL;DR: It is found that Phenol Red, a pH indicator dye commonly used in tissue culture media, had weak estrogenic activity, demonstrable by competitive binding to the estrogen receptor, stimulation of the growth rate of human breast cancer cells, and elevation of progesterone receptor levels in these cells.

TL;DR: Mixed substrate analyses showed that the dehydrogenase and isomerase reactions use the appropriate pregnene and androstene steroids as alternative, competitive substrates for product inhibition and competitive inhibition of the oxidation of pregnenolone and dehydroepiandrosterone by both product steroids, progesterone andandrostenedione.

TL;DR: The dramatic increase in E1 levels associated with the decrease in T suggests an adrenal-testicular relationship with possible potentiation of aromatization of adrenal or testicular androgens in men in septic shock.

TL;DR: Five 16-androstenes have been simultaneously quantified in extracts of the axillary hair of men using combined capillary gas chromatography-mass spectrometry, with specific ion monitoring, to indicate the existence of a pathway of metabolism in axillary bacteria in which 4,16- androstadien-3-one is reduced to 5 alpha-andstrost-16-en- 3-one and thence to the 3 alpha- and 3 beta-alcohols.

TL;DR: Only hydroxy-Flutamide was devoid of any androgenic activity and thus meets the criteria of a pure antiandrogen and was added simultaneously with each "progestin", indicating that the mitogenic effect on cell growth of all compounds is mediated by the androgen receptor.

TL;DR: Evidence in the arcuate nucleus of estrogen-induced synaptic remodelling, estrogen- induced postsynaptic membrane phenotypes, changes in intracellular membranes and rapid estrogen actions on neuronal endo-exocytosis indicates that cellular estrogen actions may underlie the neuronal control of reproduction.

TL;DR: The biological response to anti-estrogens is very variable and depends on the animal species considered, the target organ, the parameter studied, and the experimental conditions, which could explain some of the different biological effects.

TL;DR: It seems likely that the increase in plasma testosterone in male mice which reaches its peak at 2 h after birth is involved in an essential way in the development of well-documented sex differences in gonadotropin secretion and behavior.

TL;DR: TGF beta functions as a negative autocrine growth regulator and is probably responsible for some of the growth inhibitory effects of antiestrogens.

TL;DR: It is concluded that glucocorticoids most likely suppress the inflammatory response by a glucoc Corticoid receptor-mediated mechanism and under basal conditions exert tonic antiinflammatory effects.

TL;DR: A theoretical model is proposed which could explain how oestrogen and progesterone trigger the simultaneous surge of LH and prolactin.

TL;DR: The hydroxylases involved in these reactions, in the major biosynthetic tissue of ecdysone, i.e. the prothoracic glands are characterized and it is shown that C-2 hydroxyase is a mitochondrial oxygenase which differs from conventional cytochrome P-450-dependent monooxygenases by its relative insensitivity to CO.

TL;DR: The results suggest that anabolic steroids can act via muscle glucocorticoid receptors, thereby antagonizing the catabolic activity of endogenous glucOCorticoids, rather than via muscle androgen receptors.

TL;DR: The maltase activity increased significantly when undifferentiated cells were exposed to 1,25(OH)2D3 for 5-6 days, indicating that the hormone can promote differentiation of HT-29 cells.

TL;DR: The ligand binding specificity was as expected for an estrogen receptor and the sedimentation coefficient was 3.2 S when analyzed by ultracentrifugation on sucrose density gradient in presence of 0.4 M KCl and 8.1 S in low salt conditions.

TL;DR: Tamoxifen added to suspension of human endometrial adenocarcinoma cells at concentrations greater than 10 microM both increased phosphoinositide hydrolysis and inhibited the stimulatory effect of carbachol on this system, indicating nonspecific actions of the antiestrogens, shared with the structurally related phenothiazines, on the plasma membrane.

TL;DR: The use of partially purified preparations of the human uterine oestrogen receptor has enabled, for the first time, a study of the binding of the steroidal, pure antioestrogen ICI 164,384 to the oestrogens receptor.

TL;DR: The results obtained confirm that, in the rat brain, the enzymes involved in testosterone 5 alpha-reduction are preferentially localized in the white matter, but clearcut differences in the metabolic activity exist between the different structures examined so far.

TL;DR: The effects of EGF, TGF alpha and 5 alpha-dihydrotestosterone on the growth of a prostatic epithelial cell line have been evaluated in clonal growth assays and there were no significant correlations between EGF receptor concentration and tumour grade or androgen receptor content in carcinoma samples.

TL;DR: Evidence is presented utilizing eucaryotic expression of cDNA encoding the hVDR C1 domain, followed by a DNA cellulose chromatography assay, which confirms that the DNA binding activity resides in this region of the receptor for vitamin D3.

TL;DR: Exogenous and exogenous steroids may have significant effects in modulating the local formation of estrogens from androgen precursors in cultured breast carcinoma cells, demonstrating that estrogen formation may be a factor in the biological response of breast tissue.

TL;DR: The comparison of the 3D-structures of these antagonists with those of potent hormones can help to map the interaction sites with PR and GR and highlights the potential use of these molecules as labelling agents and molecular probes.

TL;DR: Investigation of the effectiveness of several imidazole drugs to inhibit human placental aromatase compared with the known inhibitors of arom atase, 4-hydroxyandrostenedione (4-OHA) and aminoglutethimide (AG) found inhibition was similar with both androstensione and testosterone as substrates.

TL;DR: The results indicate a multihormonal control of the estrogen binding protein in the kidney similar to that seen in the liver and suggest that estradiol down-regulate its own receptor.