Showing papers in "Molecular and Cellular Probes in 2000"

TL;DR: The method of bead-beating was identified as the most effective way for spore breakage and fungal DNA release and a fungus-specific PCR assay using only one primer set has been developed for detecting indoor fungi.

TL;DR: The fluorescent data analysis demonstrated that a significant higher level of fluorescent signal hence higher sensitivity of detection is obtainable using asymmetric PCR than symmetric PCR performed in presence of the molecular beacon probe.

TL;DR: Exogenous standard-based quantitative PCR was shown to be an accurate and reliable method for the quantitation of gene expression and the high internal lockup ratio of GAPDH vs VEGF copy numbers makes the use of an abundant endogenous standard an unreliable choice in quantitative or semi-quantitative PCR.

TL;DR: A multiplex PCR assay was developed which allowed the simultaneous detection of five trait genes or virulence markers in enterohemorrhagic Escherichia coli (EHEC) serotypes and confirmed the presence of these genes in a manner consistent with the known genotype of each respective strains.

TL;DR: A clear correlation is found between the intensity of gel bands and the measured probe fluorescence in solution, which suggests that the amount of PCR products can be quantified using light-up probes.

TL;DR: The present observation clearly suggests the use of PCR that amplifies the intergenic spacer region of multicopy rRNA gene of Giardia lamblia followed by nested PCR for routine, quick and reliable detection ofGiardia Lamblia in stool samples.

TL;DR: The AP-specific primers used in this study enabled a one-step identification of all isolates suitable for routine diagnosis and revealed three different subtypes named AP, AT-1 and AT-2, very closely related to apple proliferation phytoplasma.

TL;DR: GSTP1 DNA methylation status is more sensitive than telomerase analysis for the detection of malignant cells in seminal ejaculates from patients with prostate cancer, and Telomerase activity was detected in LNCaP cells recovered from normal seminal ejaculate samples.

TL;DR: The combined PCR and colorimetric reverse hybridization assay is easy to perform and faster than conventional methods for confirmation and identification of Campylobacter species.

TL;DR: PCR was shown to be the method of choice for sheep poxvirus diagnosis directly from clinical specimens and cross-reactions with orf virus were observed in three samples only in the AGIPT assay.

TL;DR: The 6.2 kb RFLP pattern, in both the poultry and human clinical isolates, indicates a common lineage for the ermA gene, which is consistent with multidrug-resistant Staphylococcus spp.

TL;DR: This method is not only rapid, simple and consistent, but also avoids a hazardous waste disposal issue associated with a previously described guanidine thiocyanate (GuSCN) extraction-PCR method.

TL;DR: Results indicate that this 'easy-to-use', rapid PCR method can accurately and reliably detect and differentiate closely related MAs avium and MAs paratuberculosis from each other and from other mycobacterial species.

TL;DR: Evaluating the use of PCR for the identification of ocular isolates of Pseudomonas aeruginosa by using primers specific to the exotoxin A gene of the bacteria found it potentially a rapid, specific, sensitive and relatively simple method.

TL;DR: Partial intragenic spacer region amplification followed by product sequence analysis offers a potentially sensitive and totally transferable means of inter- and intra-species differentiation of Bartonella strains, and its use has broadened the knowledge of the genotypic spectrum of bartonellae associated with natural infections among UK woodland rodents.

TL;DR: The presence of these two human pathogens, which are difficult to distinguish by standard biochemical methods, can now be identified using this PCR assay.

TL;DR: One pair of high-sensitive polymerase chain reaction (PCR) primers for Cryptosporidium parvum was constructed based on the sequence of random amplified polymorphic DNA, which had most advantageous in its sensitivity over the six pairs of primers reported elsewhere.

TL;DR: The serotonin receptor 2B gene (HTR2B; MIM 601122) is a pharmacological and positional candidate gene in early-onset obsessive-compulsive disorder as mentioned in this paper.

TL;DR: Three out of seven polymerases declared to possess 5'-exonuclease activity appeared to be completely unsuitable for this method while the remaining had significantly different reaction efficiencies suggesting that DNA polymerase testing is of special importance when this probe format is used.

TL;DR: The factor V status of 120 patients as determined by this method was in complete concordance with a standard PCR-based assay and clearly discriminated between healthy wild-type (+/+), factor V Leiden homozygote (−/−) and heterozygote (+/ −) carriers.

TL;DR: RP-enzyme linked immunosorbent (ELISA) assays to detect Staphylococcus aureus enterotoxins A and B genes showed overall superior detection limits and the sensitivity and specificity of the SEB PCR-ELISA was much greater than that of SEB toxin ELISA.

TL;DR: The pst probe used in this report was positive only for pesticinogenic isolates and did not show complementarity with Yersiniae nor with other bacteria targeted in this study suggesting, that the pst Probe is very specific for Y. pestis.

TL;DR: A rapid and specific method to detect filarial parasite DNAs in blood and mosquito samples using the polymerase chain reaction (PCR) technique and the restriction fragment length polymorphism (RFLP) technique.

TL;DR: The combination of PCR and non-radioactive microplate hybridization is a convenient method for the rapid detection of C. fetus.

TL;DR: The results indicate that the ESFY phytoplasma isolates affecting various Prunus species are genetically homogenous but can be distinguished from the AP phy toplasma, therefore, they are likely to represent different taxons.

TL;DR: The establishment of a multiplex PCR-based diagnostic assay applicable to identify the four species in vertebrate and invertebrate hosts and the identification of S. labiatopapillosa and F. furcata, of a canonical spliced leader 1 (SL1) sequence, so confirming that only D. repens, of the filarial parasites so far studied, shows a peculiar SL1 sequence.

TL;DR: A sudden upsurge in the isolation of Mycobacterium simiae from terminally ill AIDS patients was recently reported on the Caribbean island of Guadeloupe, and hsp65 sequence-based phylogenetic tree was created for 39 species including M. simiae and related mycobacterial species.

TL;DR: Modifications and optimization of HC-PCR are reported, particularly with respect to DNA purification from faeces, hybridization and capture steps, and procedurally sensitive critical points in the test during capture and washing of magnetic beads are identified.

TL;DR: Examination of the bacterial flora present in a chronic diabetic foot wound that failed antibiotic treatment indicates that rDNA sequencing approach can be an important tool in the identification of bacteria from wounds.

TL;DR: The construction and use of an internal control to monitor the presence of PCR inhibitors and the number of mycoplasma cells in the tracheobronchiolar washings is estimated.