Showing papers in "The Journal of Steroid Biochemistry and Molecular Biology in 2003"

TL;DR: The importance of this unique aspect of the tissue-specific regulation of aromatase expression lies in the fact that the low circulating levels of estrogens which are observed in postmenopausal women have little bearing on the concentrations of estrogen in, for example, a breast tumor, which can reach levels at least one order of magnitude greater than those present in the circulation, due to local synthesis within the breast.

TL;DR: Electrophoretic mobility shift assays revealed that the Vitamin D receptor (VDR) specifically recognized this sequence, since a VDR-DNA complex was able to compete with the unlabeled probe and was cleared by the specific anti-VDR antibody 9A7.

TL;DR: It appears that the prototype estrogen-dependent malignancy breast cancer takes advantage of four promoters that markedly increase total P450arom mRNA levels in breast cancer tissue and may upregulate aromatase expression in vascular endothelial cells.

TL;DR: Peroxisome proliferator-activated receptors are ligand-activated transcription factors belonging to the nuclear receptor superfamily that influence inflammatory cytokine production and cell recruitment to the inflammatory sites through negative interference with different inflammatory pathways.

TL;DR: The results support the concept that metabolites of estradiol may act in concert with ER mediated mechanisms to induce breast cancer and are consistent with the hypothesis that mammary tumor development is primarily initiated by metabolism of estrogens to 4-CE and, then, to CE-3,4-quinones, which may react with DNA to induce oncogenic mutations.

TL;DR: Observations of the direct effect of 4-OH-E(2) support the concept that metabolic activation of estrogens mediated by various cytochrome P450 complexes, generating through this pathway reactive intermediates that elicit direct genotoxic effects leading to transformation.

TL;DR: It is demonstrated that the mitochondria- and possibly also ER-dependent pathways are the key apoptotic pathways for heat induced germ cell death in the testis.

TL;DR: In vitro assays and chemical analysis showed that theoretical estrogenic activity expressed as equivalent E2 concentration is in the same range as recommended for synthetic estrogens.

TL;DR: The molecular analyses of five familial and three sporadic cases of the familial/sporadic glucocorticoid resistance syndrome are reviewed and the possible contribution of newly identified molecules, such as HIV-1 accessory proteins Vpr and Tat, FLICE-associated huge protein (FLASH) and chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII), on the molecular regulation of GR activity are discussed.

TL;DR: The metabolic pathways of daidzein, genistein and glycitein are presented on the basis of the identification of the metabolites in human urine after soy supplementation.

TL;DR: Data support the hypothesis that ginsenoside-Rh1 acts as a weak phytoestrogen, presumably by binding and activating the estrogen receptor, in CV-1 cells transiently transfected with corresponding steroid hormone receptors and hormone responsive reporter plasmids.

TL;DR: The hypothesis that transcripts for steroidogenic enzymes are expressed in fetal tissues other than the classical steroidogenic organs are tested and real-time reverse transcription polymerase chain reaction (RT-RTPCR) assays were developed suggest that RT- RTPCR is a powerful tool for the examination of steroidogenic enzyme mRNA expressions.

TL;DR: Letrozoles appears to have a small effect on adrenal steroidogenesis such that a small number of patients exhibit an abnormal response to synthetic ACTH during letrozole therapy, which is unlikely to have any clinical significance.

TL;DR: Preliminary evidence suggests a role for treatment in pre-menopausal women with symptoms and lowered testosterone levels, and debate exists as per importance of measurement of free versus total testosterone, with the "free androgen index" offering an alternative method of assessment of testosterone availability.

TL;DR: Preliminary results from this study suggest that anti-oestrogen therapy may cause a specific deficit in verbal memory that corroborates the links between oestrogen levels and verbal memory often reported in studies of the cognitive benefits of hormone replacement therapy.

TL;DR: Postmenopausal breast tissue has the ability to maintain concentrations of estrone and estradiol that are 2-10- and 10-20-fold higher than the corresponding plasma estrogen levels, which may be explained by uptake of estrogens from the circulation and/or local estrogen production.

TL;DR: In vivo and in vitro studies indicate that the oxidation of LDL isolated from postmenopausal women was inhibited differently by various estrogens and other antioxidants, and the unique ring B unsaturated estrogens were the most potent while the red wine component t-resveratrol was the least potent.

TL;DR: A protein, CCCP, is identified, which binds to the C-terminal region of CARM1 and cooperates synergistically withCARM1 to enhance NR function and binds a novel coactivator called coiled-coil coActivator (CoCoA), which acts synergistic with p160 coactivators to enhanceNR function.

TL;DR: Zebrafish cyp19 encodes P450 aromatase, the key enzyme catalyzing the conversion of androgens into estrogens, and sequences for recognition of chicken ovalbumin upstream promoter-transcription factor (COUP) and Ptx-1 were detected in the promoter.

TL;DR: Using in vitro models, numerous regulatory links between signal transduction pathways elicited by peptide growth factors and PR are established, suggesting a mechanism for steroid hormone receptor "hypersensitivity" induced by growth factors.

TL;DR: Evidence is provided for a varying expression of ERα, ERβ and PR in bovine oviducts at different cycle stages in vivo, respectively under steroid supplementation in vitro, which point towards a functional importance of the three steroid receptors in the boviduct, the site of fertilization and early embryonic development.

TL;DR: Based on its superiority on the human enzyme, Sa 40 in its 3beta-acetate form (Sa 41) could be a promising candidate for clinical evaluation.

TL;DR: A new molecular mechanism whereby phospho-GATA factors contribute to increased transcription of steroidogenic genes in response to hormonal stimulation is defined.

TL;DR: Using a mammalian cell-based transient transactivationassay, it is demonstrated that 8-PN is the strongest plant-derived ER agonist identified so far, being about 10times more potent than coumestrol and approximately 100times more powerful than genistein.

TL;DR: Analysis of AR-mediated transcription suggest that the orchestrated interaction of AR with the transcription factors IIF and IIH, and positive transcription elongation factor b, may increase efficiency of transcriptional elongation from the androgen target genes, such as prostate specific antigen (PSA).

TL;DR: The present study shows that TPT acts as an unspecific, but significant inhibitor of human sex steroid hormone metabolism and suggests that the inhibitory effects are mediated by the interaction of TPT with critical cysteine residues of the enzymes.

TL;DR: The RT-PCR method concludes that the effects of silymarin in this part of the bone cannot be exerted via the ERalpha because it does not bind to this receptor subtype.

TL;DR: Phenotypic analysis has provided definitive experimental findings for estrogen receptor mediated physiological actions, involving ERalpha in uterine, mammary gland and neuroendocrine sites and ERbeta in ovary as is ERalpha.

TL;DR: Fast changes in the local availability of estrogens in the brain can be caused by aromatase phosphorylation so that estrogen could rapidly regulate neuronal physiology and behavior.

TL;DR: The alterations in cell counts, HDL-cholesterol, liver function and most hormones of the pituitary-testicular axis induced by a long-term abuse of AAS were reversible after stopping the medication for over 1 year.