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Showing papers in "Veterinary Microbiology in 1999"


Journal ArticleDOI
TL;DR: Estimates of economic losses due to BVDV infection vary depending on the immune status of the population and the pathogenicity of the infecting virus strains, and cost-benefit analyses of control programs are highly dependent on the risks of new infections under different circumstances and on the strains of the virus involved.

666 citations


Journal ArticleDOI
TL;DR: Important risk factors for spreading BVDV are discussed, where livestock trade is perceived as the most central to control, and the importance of making farmers aware of herd biosecurity and their own responsibility for it is stressed.

363 citations


Journal ArticleDOI
TL;DR: A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates, which were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease, but distinct from the isolate from continuous PK/15 cell cultures.

198 citations


Journal ArticleDOI
TL;DR: R reverse transcription, coupled with the polymerase chain reaction (RT/PCR) and nucleic acid sequencing, has been used to characterise the morbilliviruses and has given insights into the evolution of this virus genus.

161 citations


Journal ArticleDOI
TL;DR: The data point to the high prevalence of Shiga toxin-producing Escherichia coli (STEC) strains in the authors' environment and suggest the need for good control strategies for the prevention of contamination of animal products.

155 citations


Journal ArticleDOI
TL;DR: There is ample room for improvement of both the efficacy and safety of BVDV vaccines, and it may be expected that better vaccines, among which marker vaccines, will be launched in the future.

150 citations


Journal ArticleDOI
TL;DR: A monoclonal antibody that reacted specifically with a 16 kDa big liver and spleen disease virus (BLSV) protein was used to identify the protein in western immunoblots of infected liver extracts and enable partial amino acid sequence analysis of the protein.

145 citations


Journal ArticleDOI
TL;DR: Findings indicate that the E2 marker vaccine merits further evaluation for suitability for use in a control program during an outbreak of CSF.

144 citations


Journal ArticleDOI
TL;DR: Five porcine finishing units, previously identified as contaminated by Salmonella, were sampled to identify possible sources of contamination and to study the distribution ofSalmonella within the herds to suggest a vertical contamination throughout the different production steps.

144 citations


Journal ArticleDOI
TL;DR: Automated liquid feeding of by-products, and membership of an Integrated Quality Control (IQC) production group were associated with a decreased risk of infection, while use of trough feeding was associated with an increasedrisk of infection.

141 citations


Journal ArticleDOI
TL;DR: In this paper, the authors investigated whether bovine lymphocytes undergo DNA fragmentation during LKT-induced apoptosis and showed that LKT at low concentrations induced apoptotic cell death.

Journal ArticleDOI
TL;DR: An important finding of this study was that while five of 24 C. pecorum-infected koalas had clinical signs of the disease (both ocular and urogenital sites), none out of seven C. pneumoniae-infecting koALas had signs of clinical disease, suggesting that C.pecorum may be the more pathogenic of the two chlamydial species infecting this host.

Journal ArticleDOI
TL;DR: Screening of sera from other herds demonstrates that swine HEV is present in Australia, with reactivity observed in 30% of random samples from two piggeries, and 92-95% of pigs by the age of 16 weeks in two other piggeries and 17% of wild-caught pigs.

Journal ArticleDOI
TL;DR: Results indicate that PRRSV GP5 in principle is a major target for neutralizing antibodies, as is found for other arteriviruses, but that in nature 'ORF 5 escape mutants' may develop as easily as in vitro.

Journal ArticleDOI
TL;DR: A reverse-transcription polymerase chain reaction (RT-PCR) was developed to differentiate the bovine diarrhea virus (BVDV) from other pestiviruses, and to determine the genotype of the BVDV isolates.

Journal ArticleDOI
TL;DR: Five different molecular typing methods were used to analyse isolates of Staphylococcus aureus obtained from milk samples of dairy cows suffering from subclinical mastitis in southern Brazil for the determination of their in-vitro susceptibility to 14 different antibiotics.

Journal ArticleDOI
TL;DR: Of the strains isolated from diseased pigs, 264 (71.0%) were negative for the fimbrial antigens (genes) examined in this study, and fimbria-negative isolates frequently possessed genetic determinants for LTI or LTI and STI or for STII enterotoxins.

Journal ArticleDOI
TL;DR: Within ORF1, there was complete conservation of the putative nucleotide binding site and marked conservation of 2 other motifs previously identified as essential components of the replication-associated proteins of other circoviruses and geminiviruses.

Journal ArticleDOI
TL;DR: The anti-colonization effect of porcine plasma powder against experimentally induced postweaning diarrhoea and oedema disease in just weaned piglets was examined and protection against clinical symptoms was obtained.

Journal ArticleDOI
TL;DR: Cattle arriving for slaughter at a large abattoir in northern Italy between April 1997 and January 1998 were examined for intestinal carriage of Verocytotoxin-producing Escherichia coli (VTEC) O157 using an immunomagnetic separation technique, observing typical biochemical features in some VTEC O157 strains.

Journal ArticleDOI
TL;DR: Airborne transmission of bovine herpesvirus 1, BRSV and BVDV showed that all the three viruses were transmitted by air, and these findings will have consequences for disease prevention and regulations in control programmes.

Journal ArticleDOI
TL;DR: Sequence analysis has revealed that CPV strains have undergone a series of evolutionary selections in nature which have resulted in the global distribution of new virus variants.

Journal ArticleDOI
TL;DR: The results indicated that the aesculin-hydrolyzing cocci associated with this type of infection are a very heterogenic group.

Journal ArticleDOI
TL;DR: The NADH oxidase genes of 18 strains of intestinal spirochaetes were partially sequenced and a phenogram produced based on sequence dissimilarities was in good agreement with the current classification of species in the genus Serpulina, although an atypical strongly beta-haemolytic porcine strain appeared distinct from other members of this species.

Journal ArticleDOI
TL;DR: The epidemiological significance of subclinical infection and the extended BT virus viraemias in Lesbos sheep and goats, in relation to the maintenance of the virus and to overwintering is discussed.

Journal ArticleDOI
TL;DR: Findings show, that single, widely distributed clones seemed to be responsible for cases of bovine subclinical mastitis found in one region of Germany.

Journal ArticleDOI
TL;DR: The results of these studies support the long standing management practices of separating pregnant mares from other groups of horses to reduce the incidence of EHV-1 abortion and have important implications for currently recommended vaccination regimens.

Journal ArticleDOI
TL;DR: Strain I2 virus at seed and vaccine level induced adequate levels of antibody in chickens vaccinated by eye drop and usually in their contacts, and is suitable for local production of thermostable vaccine in regional laboratories in developing countries.

Journal ArticleDOI
TL;DR: The results indicate that BVDV infections can reliably be diagnosed by the Ceditest ELISA and that the test is suitable for use in large scale screening and eradication programmes.

Journal ArticleDOI
TL;DR: These calves did not exhibit persistent low-level shedding, and did not appear to be persistently colonized with E. coli O157:H7.