Journal ArticleDOI
A graphical method of determining the michaelis-menten constant free of external mass transfer resistance for immobilized enzymes in a packed bed reactor
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In this paper, a graphical method of determining the Michaelis-Menten constant free of the external mass transfer resistance for a packed bed immobilized enzyme system was illustrated with examples from three different enzyme reactions.Abstract:
A graphical method of determining the Michaelis-Menten constant free of the external mass transfer resistance for a packed bed immobilized enzyme system was illustrated with examples from 3 different enzyme reactions. The intercept at the ordinate obtained by the straight line extrapolation of data points in the plot of apparent Km value vs. the reciprocal of superficial velocity in column allowed an easy calculation of Km free of external mass transfer resistance. An asymptotic value of apparent Km value at infinite zero superficial velocity was ascribed to the fact that the mass transfer coefficient kL, approached a definite value at this condition.read more
Citations
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Optimal immobilization of β-galactosidase from Pea (PsBGAL) onto Sephadex and chitosan beads using response surface methodology and its applications
Alka Dwevedi,Arvind M. Kayastha +1 more
TL;DR: Response surface methodology (RSM) and centre composite design (CCD) were used to optimize immobilization of beta-galactosidase (BGAL) from Pisum sativum onto two matrices: Sephadex G-75 and chitosan beads.
Journal ArticleDOI
Acetylcholinesterase voltammetric biosensors based on carbon nanostructure-chitosan composite material for organophosphate pesticides
Alina Catrinel Ion,Ion Ion,Alina Culetu,Dragos Gherase,Carmen Moldovan,Rodica Iosub,Adrian Dinescu +6 more
TL;DR: In this paper, a sensitive biosensor for chloropyrifos (CPF), an organophosphorus pesticide, was developed by immobilizing acetylcholinesterase (AChE) through covalent bonding to an oxidized exfoliated graphite nanoplatelet (xGnPs)-chitosan cross-linked composite.
Journal ArticleDOI
Stabilization of beta-galactosidase (from peas) by immobilization onto amberlite MB-150 beads and its application in lactose hydrolysis.
Alka Dwevedi,Arvind M. Kayastha +1 more
TL;DR: The easy accessibility of enzyme source, ease of its immobilization on Amberlite, lower cost of Amber Lite, enhanced stability of Amberlite-PsBGAL, and comparable lactose hydrolysis in milk and milk whey described here make it a suitable product for future applications at laboratory and industrial scale.
Journal ArticleDOI
Mass transfer limitations in immobilized cells.
TL;DR: Article de synthese sur les transferts de masse interphase et intraphase; performance de reacteurs a cellules immobilisees
Journal ArticleDOI
Effect of mass transfer in a recirculation batch reactor system for immobilized penicillin amidase.
TL;DR: The effect of external mass transfer resistance on the overall reaction rate of the immobilized whole cell penicillin amidase of E. coli in a recirculation batch reactor was investigated and the correlation proposed by Chilton and Colburn represented adequately the experimental data.
References
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Journal ArticleDOI
The isolation and kinetics of penicillin amidase from Escherichia coli
TL;DR: The enzyme was shown to be inhibited by excess substrate, benzylpenicillin, and by both of the products of hydrolysis, which was found to be competitive and by 6-aminopenicillanic acid to be non-competitive.
Journal ArticleDOI
The kinetics of carboxymethylcellulose-ficin in packed beds
Lilly,WE Hornby,EM Crook +2 more
TL;DR: The kinetics of the hydrolysis of benzoylarginine ethyl ester in packed columns of CM-cellulose-70-ficin and CM-cells-90- ficin were studied and the apparent Michaelis constant was calculated and shown to be dependent on the flow rate at low rates of perfusion through the columns.
Journal ArticleDOI
Mass and heat transfer to single spheres and cylinders at low Reynolds numbers
TL;DR: In this article, the velocity distributions which are assumed to exist are those derived from the linearized Navier-Stokes equations by Tomotika and Aoi, and experimental data for heat and mass transfer to single cylinders at large NPe check the theory.
Journal ArticleDOI
Some changes in the reactivity of enzymes resulting from their chemical attachment to water-insoluble derivatives of cellulose
TL;DR: The apparent K(m) with respect to ATP and Mg(2+) of ATP-creatine phosphotransferase was observed to increase about tenfold on attachment of the enzyme to CM-cellulose, and to increase by only 23% on its attachment to p-aminobenzyl cellulose.
Journal ArticleDOI
Effects of transpiration and changing diameter on heat and mass transfer to spheres
P. L. T. Brian,H. B. Hales +1 more
TL;DR: In this paper, the effects of a transpiration velocity at the surface of a sphere and of a continuously changing sphere diameter are included, and theoretical corrections for transpiring and shrinking spheres are applied to the mass transfer data for ice spheres that are melting in an agitated brine bath.
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