A Mutant of Bacteriophage T7 Deficient in Polynucleotide Ligase
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TLDR
Small fragments of DNA accumulate after T7amHA13 infection of N1318, suggesting that the T7 ligase plays a role in DNA replication, but that the wild type host can supply sufficient ligase activity for growth of phage T7.About:Â
This article is published in Journal of Biological Chemistry.The article was published on 1971-11-25 and is currently open access. It has received 88 citations till now. The article focuses on the topics: Ligase activity & DNA ligase.read more
Citations
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Bacteriophage T7: Genetic and biochemical analysis of this simple phage gives information about basic genetic processes.
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Refactoring bacteriophage T7
TL;DR: The viability of the initial design suggests that the genomes encoding natural biological systems can be systematically redesigned and built anew in service of scientific understanding or human intention.
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Gene 0.3 of bacteriophage T7 acts to overcome the DNA restriction system of the host
TL;DR: In the course of searching for T7 mutants that are unable to overcome restriction, it was discovered that mutants defective in gene 2 are able to plate on E. coli C with essentially normal efficiency, and most gene 7 mutants are ableto plate on both C and K strains.
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DNA ligases in the repair and replication of DNA.
TL;DR: Sequence and structural analysis of DNA ligases has shown that these enzymes are built around a common catalytic core, which is likely to be similar in three-dimensional structure to that of T7-bacteriophage ligase.
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Genetic analysis of non-essential bacteriophage T7 genes.
TL;DR: Isolation and genetic characterization of a series of deletions and point mutants affecting two non-essential genes of bacteriophage T7 is described and host strains were found in which one or both of these T7 genes is required for growth.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Isolation of an E. coli strain with a mutation affecting DNA polymerase.
Paula De Lucia,John Cairns +1 more
TL;DR: By testing indiscriminately several thousand colonies of mutagenized E. coli, a mutant has been isolated that on extraction proves to have less than I per cent of the normal level of DNA polymerase.
Journal ArticleDOI
New RNA polymerase from Escherichia coli infected with bacteriophage T7.
TL;DR: A new T7-specific RNA polymerase is found in T7 phage-infected cells and provides a direct explanation for the pleiotropic control of late T7 functions exerted by gene I.
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The genetics and physiology of bacteriophage T7
TL;DR: It appears that in T7 (as in T4 and lambda) related functions tend to cluster along the genetic map, which makes this system a very attractive one in which to study a number of biological problems at the molecular level.
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Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage.
TL;DR: The purification and some properties of an enzyme system, polynucleotide ligase, which catalyzes the repair of single-strand breaks by the formation of phosphodiester bonds are described.