Journal ArticleDOI
A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochrome-labelled RNA
TLDR
A new method has been developed for the detection of in situ hybridization by fluorescence microscopy based on the covalent binding of commercially available fluorochromes to the 3′-terminus of RNA.About:
This article is published in Experimental Cell Research.The article was published on 1980-08-01. It has received 311 citations till now. The article focuses on the topics: Hybridization probe & In situ hybridization.read more
Citations
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Journal ArticleDOI
Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.
TL;DR: The use of fluorescence in situ hybridization for chromosome classification and detection of chromosome aberrations is described and chromosomes in human-hamster hybrid cell lines were intensely and uniformly stained in metaphase spreads and interphase nuclei when human genomic DNA was used as a probe.
Journal ArticleDOI
Immunological method for mapping genes on Drosophila polytene chromosomes
TL;DR: This immunological approach offers four advantages over conventional autoradiographic procedures for detecting in situ hybrids: the time required to determine the site of hybridization is decreased markedly, the biotin-labeled probes are chemically stable and give reproducible results for many months, and the resolving power is equal to and often greater than that achievedautoradiographically.
Journal ArticleDOI
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes
David J. Brigati,David Myerson,David Myerson,Jeffry J. Leary,Barbara Spalholz,Susan Z. Travis,Caroline K.Y. Fong,Caroline K.Y. Fong,G. D. Hsiung,G. D. Hsiung,David C. Ward +10 more
TL;DR: The visualization of parvov virus, polyomavirus, herpes simplex virus, adenovirus, and retrovirus genetic material in infected cell cultures and herpessimplex and adenvirus DNA in paraffin-embedded autopsy tissues is reported.
Patent
Modified nucleotides and methods of preparing and using same
TL;DR: In this paper, the structure of polynucleotide sequences in chromosomes, fixed cells, tissue sections, and cell extracts has been studied for detection and localization of DNA-RNA sequences.
Journal ArticleDOI
Fluorescence in situ hybridization: past, present and future.
TL;DR: Key methodological advances have allowed facile preparation of low-noise hybridization probes, and technological breakthroughs now permit multi-target visualization and quantitative analysis - both factors that have made FISH accessible to all and applicable to any investigation of nucleic acids.
References
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Journal ArticleDOI
A new technique for the assay of infectivity of human adenovirus 5 DNA.
Frank L. Graham,A. J. Van Der Eb +1 more
TL;DR: A new technique for assaying infectivity of adenovirus 5 DNA has been developed and a reproducible relationship between amounts of DNA inoculated per culture and numbers of plaques produced was demonstrated.
Journal ArticleDOI
RNA-DNA Hybrids at the Cytological Level
TL;DR: Radioactive RNA introduced into “target” cells can be induced to form hybrids with nuclear DNA and the location of these hybrids can be detected by autoradiography.
Journal ArticleDOI
Quantitative single cell analysis and sorting.
Paul Karl Horan,Leon L. Wheeless +1 more
Journal ArticleDOI
High resolution detection of DNA-RNA hybrids in situ by indirect immunofluorescence.
George T. Rudkin,B D Stollar +1 more
TL;DR: A new method for the detection of RNA–DNA hybrids in cytological preparations is described with significant advantages over the autoradiographic procedures used so far, and the locations of hybrid molecules on polytene chromosomes are revealed.
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Formation and detection of rna-dna hybrid molecules in cytological preparations
Joseph G. Gall,Mary Lou Pardue +1 more