Journal ArticleDOI
A rapid and efficient procedure for the purification of DNA from agarose gels.
TLDR
DNA fragments electrophoresed through a horizontal agarose slab gel can be recovered by inserting strips of filter paper backed by dialysis membrane into slits cut in the gel in front of the DNA bands and continuing Electrophoresis until the DNA is collected in the paper.About:
This article is published in Analytical Biochemistry.The article was published on 1980-08-01. It has received 154 citations till now. The article focuses on the topics: Gel electrophoresis of nucleic acids & Agarose.read more
Citations
More filters
Journal ArticleDOI
Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
TL;DR: The LiAc/SS‐DNA/PEG method was shown to be more effective than other treatments known to make cells transformable and caused tighter binding of 32P‐labelled plasmid DNA than did double‐stranded (DS) carrier.
Journal ArticleDOI
A reliable method for the recovery of DNA fragments from agarose and acrylamide gels
TL;DR: The method is useful for the recovery of biologically active DNA from both agarose and high-percentage polyacrylamide gels, thereby enabling purification of DNA fragments up to 20 kb in size with a yield of 60–80%.
Journal ArticleDOI
Pichia pastoris as a host system for transformations.
TL;DR: A methylotrophic yeast, Pichia pastoris, is developed as a host for DNA transformations based on an auxotrophic mutant host of P. pastoris which is defective in histidinol dehydrogenase.
Journal ArticleDOI
Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier.
TL;DR: Co‐transformation showed that 30–40% of the transformation‐competent cells take up more than one DNA molecule which can be used to enrich for integration and delection events 30‐ to 60‐fold.
Journal ArticleDOI
Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris
TL;DR: Two DNA fragments containing putative control regions regulating the expression of the alcohol oxidase and dihydroxy-acetone synthase genes from the methylotrophic yeast Pichia pastoris were used in the construction of vectors for theexpression of the Escherichia coli lacZ gene.
References
More filters
Journal ArticleDOI
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
Journal ArticleDOI
A new technique for the assay of infectivity of human adenovirus 5 DNA.
Frank L. Graham,A. J. Van Der Eb +1 more
TL;DR: A new technique for assaying infectivity of adenovirus 5 DNA has been developed and a reproducible relationship between amounts of DNA inoculated per culture and numbers of plaques produced was demonstrated.
Journal ArticleDOI
Size and location of the transforming region in human adenovirus type 5 DNA
TL;DR: Fragments of human adenovirus type 5 DNA as small as one million daltons can transform cells in vitro.
Journal ArticleDOI
A method for the recovery of DNA from agarose gels
H.F. Tabak,R.A. Flavell +1 more
TL;DR: A quick and versatile method for the isolation of DNA from agarose gels by putting the hydroxyapatite on a small column of Sephadex G50, elution and subsequent removal of phosphate can be performed in one step.
Journal ArticleDOI
A rapid method for extracting DNA from agarose gels.
Mark Finkelstein,Robert H. Rownd +1 more
TL;DR: The DNA obtained with an agarase extraction procedure is physically intact and biologically active and should be useful for a wide range of applications.
Related Papers (5)
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more