Journal ArticleDOI
A restriction enzyme from Hemophilus influenzae: II. Base sequence of the recognition site
TLDR
In this paper, the authors have explored the nucleotide sequences at the 5′-ends of the limit product by labeling the 5-phoryl groups (using polynucleotide kinase) and characterizing the labeled fragments released by various nucleases.About:
This article is published in Journal of Molecular Biology.The article was published on 1970-07-28. It has received 518 citations till now. The article focuses on the topics: Restriction enzyme & Endonuclease.read more
Citations
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis
Journal ArticleDOI
The CRISPR tool kit for genome editing and beyond.
TL;DR: A brief history of gene-editing tools is presented and the wide range of CRISPR-based genome-targeting tools are described, to conclude with future directions and the broader impact ofCRISPR technologies.
Journal ArticleDOI
Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
Mamie Z. Li,Stephen J. Elledge +1 more
TL;DR: A new cloning method, sequence and ligation–independent cloning (SLIC), which allows the assembly of multiple DNA fragments in a single reaction using in vitro homologous recombination and single-strand annealing, which allows much greater versatility in the generation of recombinant DNA for the purposes of synthetic biology.
Journal ArticleDOI
A Restriction enzyme from Hemophilus influenzae: I. Purification and general properties
H O Smith,K W Wilcox +1 more
TL;DR: Extracts of Hemophilus influenzae strain Rd contain an endonuclease activity which produces a rapid decrease in the specific viscosity of a variety of foreign native DNA's; this enzyme was purified approximately 200-fold.
References
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Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
On the Nature of Allosteric Transitions: A Plausible Model
TL;DR: "It is certain that all bodies whatsoever, though they have no sense, yet they have perception, and whether the body be alterant or alterec, evermore a perception precedeth operation; for else all bodies would be like one to another."
Journal ArticleDOI
Sedimentation studies of the size and shape of dna.
TL;DR: Analytical zone sedimentation ( Vinograd, Bruner, Kent & Weigle, 1963) is shown to be a sensitive and reliable method for detecting differences in conformation and for determining the molecular weight of homogeneous phage DNA's.
Journal ArticleDOI
A two-dimensional fractionation procedure for radioactive nucleotides
TL;DR: High-voltage ionophoresis is used in both dimensions for the two-dimensional fractionation of ribonuclease digests of 32P-labelled RNA and the determination of the sequence of a nucleotide by partial digestion with spleen phosphodiesterase.
Journal ArticleDOI
DNA Restriction Enzyme from E. coli
Matthew Meselson,Robert Yuan +1 more
TL;DR: An endonuclease which degrades foreign DNA has been isolated and requires S-adenosylmethionine, ATP and Mg++.
Related Papers (5)
A Restriction enzyme from Hemophilus influenzae: I. Purification and general properties
H O Smith,K W Wilcox +1 more