Journal ArticleDOI
A two-dimensional fractionation procedure for radioactive nucleotides
TLDR
High-voltage ionophoresis is used in both dimensions for the two-dimensional fractionation of ribonuclease digests of 32P-labelled RNA and the determination of the sequence of a nucleotide by partial digestion with spleen phosphodiesterase.About:
This article is published in Journal of Molecular Biology.The article was published on 1965-09-01. It has received 993 citations till now. The article focuses on the topics: Pancreatic ribonuclease & Ribonuclease T1.read more
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The 3′-Terminal Sequence of Escherichia coli 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites
John Shine,Lynn Dalgarno +1 more
TL;DR: It is suggested that this region of the RNA is able to interact with mRNA and that the 3'-terminal U-U-A(OH) is involved in the termination of protein synthesis through base-pairing with terminator codons.
Journal ArticleDOI
Methanogens: reevaluation of a unique biological group.
TL;DR: The present study focuses on the development and outline of a new treatment based on 16-year-old ribonucleic acid, as well as evidence in support of the new taxonomic treatment.
Journal ArticleDOI
The Phylogeny of Prokaryotes
George E. Fox,Erko Stackebrandt,R.B. Hespell,Jane Gibson,J Maniloff,TA Dyer,Ralph S. Wolfe,William E. Balch,Ralph S. Tanner,Linda J. Magrum,L. Zablen,R Blakemore,Ramesh Gupta,L. Bonen,B. J. Lewis,David A. Stahl,Kenneth R. Luehrsen,KN Chen,Carl R. Woese +18 more
TL;DR: For the first time, a single experimental approach, 16S ribosomal RNA sequence characterization, has been used to develop an overview of phylogenetic relationships in the bacterial world as mentioned in this paper.
Journal ArticleDOI
Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes
TL;DR: A strong positive correlation between the tRNA abundance and the choice of codons, among both synonymous codons and those corresponding to different amino acids, was found for all E. coli protein genes that had been sequenced completely; the correlation was less significant for the phage genes.
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The sequence of sequencers: The history of sequencing DNA.
TL;DR: This article traverses those years, iterating through the different generations of sequencing technology, highlighting some of the key discoveries, researchers, and sequences along the way.
References
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A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. Coli I. Purification and characterization of alkaline phosphatase
A. Garen,C. Levinthal +1 more
TL;DR: A procedure for purification of alkaline phosphatase from E. coli is described, and several physical-chemical propetiies of the purified enzyme are reported.
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The structure of ribonucleic acids. 1. Cyclic nucleotides produced by ribonuclease and by alkaline hydrolysis
R. Markham,J. D. Smith +1 more
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The amino-acid sequence in the phenylalanyl chain of insulin. 1. The identification of lower peptides from partial hydrolysates
Frederick Sanger,H. Tuppy +1 more
TL;DR: It was concluded that all the N-terminal phenylalanyl residues of insulin and all the lysine residues were present in the above two sequences respectively, and hence that there was only one type of phenyl alanyl polypeptide chain in insulin.