A Rice Brassinosteroid-Deficient Mutant, ebisu dwarf (d2), Is Caused by a Loss of Function of a New Member of Cytochrome P450
Zhi Hong,Miyako Ueguchi-Tanaka,Kazuto Umemura,Sakurako Uozu,Shozo Fujioka,Suguru Takatsuto,Shigeo Yoshida,Motoyuki Ashikari,Hidemi Kitano,Makoto Matsuoka +9 more
TLDR
It is concluded that D2/CYP90D2 catalyzes the steps from 6-deoxoteasterone to 3-dehydro-6- deoxoteastersone and from teasteroneto 3- dehydroteasterone in the late BR biosynthesis pathway.Abstract:
We characterized a rice dwarf mutant, ebisu dwarf (d2). It showed the pleiotropic abnormal phenotype similar to that of the rice brassinosteroid (BR)-insensitive mutant, d61. The dwarf phenotype of d2 was rescued by exogenous brassinolide treatment. The accumulation profile of BR intermediates in the d2 mutants confirmed that these plants are deficient in late BR biosynthesis. We cloned the D2 gene by map-based cloning. The D2 gene encoded a novel cytochrome P450 classified in CYP90D that is highly similar to the reported BR synthesis enzymes. Introduction of the wild D2 gene into d2-1 rescued the abnormal phenotype of the mutants. In feeding experiments, 3-dehydro-6-deoxoteasterone, 3-dehydroteasterone, and brassinolide effectively caused the lamina joints of the d2 plants to bend, whereas more upstream compounds did not cause bending. Based on these results, we conclude that D2/CYP90D2 catalyzes the steps from 6-deoxoteasterone to 3-dehydro-6-deoxoteasterone and from teasterone to 3-dehydroteasterone in the late BR biosynthesis pathway.read more
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Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice.
Tomoaki Sakamoto,Yoichi Morinaka,Toshiyuki Ohnishi,Hidehiko Sunohara,Shozo Fujioka,Miyako Ueguchi-Tanaka,Masaharu Mizutani,Kanzo Sakata,Suguru Takatsuto,Shigeo Yoshida,Hiroshi Tanaka,Hidemi Kitano,Makoto Matsuoka +12 more
TL;DR: It is shown that the erect leaf phenotype of a rice brassinosteroid–deficient mutant, osdwarf4-1, is associated with enhanced grain yields under conditions of dense planting, even without extra fertilizer, suggesting that regulated genetic modulation of brassinosterone biosynthesis can improve crops without the negative environmental effects of fertilizers.
Journal ArticleDOI
Molecular Basis of Plant Architecture
Yonghong Wang,Jiayang Li +1 more
TL;DR: The identification of mutants that are defective in plant architecture and characterization of the corresponding and related genes will eventually enable us to elucidate the molecular mechanisms underlying plant architecture, one of the hottest areas in plant developmental biology.
Journal ArticleDOI
A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length.
Sumiyo Tanabe,Motoyuki Ashikari,Shozo Fujioka,Suguru Takatsuto,D. Shigeo Yoshida,Masahiro Yano,Atsushi Yoshimura,Hidemi Kitano,Makoto Matsuoka,Yukiko Fujisawa,Hisaharu Kato,Yukimoto Iwasaki +11 more
TL;DR: Multiple lines of evidence together suggest that the D11/CYP724B1 gene plays a role in BR synthesis and may be involved in the supply of 6-DeoxoTY and TY in the BR biosynthesis network in rice.
Journal ArticleDOI
Brassinosteroids: Multidimensional Regulators of Plant Growth, Development, and Stress Responses.
Trevor M. Nolan,Nemanja Vukašinović,Nemanja Vukašinović,Derui Liu,Derui Liu,Eugenia Russinova,Eugenia Russinova,Yanhai Yin +7 more
TL;DR: Recent progress toward understanding theBR pathway is summarized, including BR perception and the molecular mechanisms of BR signaling, and how knowledge of the BR pathway is being applied to manipulate the growth and stress responses of crops is shown.
Journal ArticleDOI
DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously
Xiang-Jin Wei,Jun-Feng Xu,Hongnian Guo,Ling Jiang,Saihua Chen,Chuanyuan Yu,Zhen-ling Zhou,Peisong Hu,Huqu Zhai,Jianmin Wan +9 more
TL;DR: Qualitative real-time PCR assay data indicate that DTH8 probably plays an important role in the signal network of photoperiodic flowering as a novel suppressor as well as in the regulation of plant height and yield potential.
References
More filters
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
P450 superfamily: Update on new sequences, gene mapping, accession numbers and nomenclature
David R. Nelson,Luc Koymans,Tetsuya Kamataki,John J. Stegeman,Ren Feyereisen,David J. Waxman,Michael R. Waterman,Osamu Gotoh,Minor J. Coon,Ron W. Estabrook,Irwin C. Gunsalus,Daniel W. Nebert +11 more
TL;DR: This revision supersedes the four previous updates in which a nomenclature system, based on divergent evolution of the P450 superfamily has been described and is similar to that proposed in the previous updates.
Journal ArticleDOI
‘Green revolution’ genes encode mutant gibberellin response modulators
Jinrong Peng,Donald Ernest Richards,Nigel M. Hartley,George Murphy,Katrien M. Devos,John E. Flintham,James Beales,L. J. Fish,A. J. Worland,Fatima Pelica,Duraialagaraja Sudhakar,Paul Christou,John W. Snape,Michael D. Gale,Nicholas P. Harberd +14 more
TL;DR: It is shown that Rht-B1/Rht-D1 and maize dwarf-8 (d8), are orthologues of the Arabidopsis Gibberellin Insensitive (GAI) gene, which encode proteins that resemble nuclear transcription factors and contain an SH2-like domain, indicating that phosphotyrosine may participate in gibberelli signalling.
Journal ArticleDOI
A Putative Leucine-Rich Repeat Receptor Kinase Involved in Brassinosteroid Signal Transduction
Jianming Li,Joanne Chory +1 more
TL;DR: The identification of 18 Arabidopsis dwarf mutants that are unable to respond to exogenously added brassinosteroid, a phenotype that might be expected for brass inosteroid signaling mutants.
Journal ArticleDOI
BRASSINOSTEROIDS: Essential Regulators of Plant Growth and Development
TL;DR: This review examines the microchemical and molecular genetic analyses that have provided convincing evidence for an essential role of BRs in diverse developmental programs, including cell expansion, vascular differentiation, etiolation, and reproductive development.