Journal ArticleDOI
A sensitive and specific DNA probe to identify enteroaggregative Escherichia coli, a recently discovered diarrheal pathogen.
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TLDR
The isolation and cloning of a 1-kilobase fragment from the plasmid ofEAggEC strain 17-2 is described, which should greatly facilitate epidemiologic studies assessing the importance of EAggEC as a diarrheal pathogen.Abstract:
The epidemiologic significance of enteroaggregative Escherichia coli (EAggEC) as a diarrheal pathogen has only recently come under study. Although EAggEC has been associated with persistent diarrhea in infants in some developing countries, additional studies are clearly needed. Until now, the only means of identifying EAggEC strains has been the cumbersome HEp-2 cell adhesion assay. The isolation and cloning of a 1-kilobase fragment from the plasmid of EAggEC strain 17-2 is described. This probe is 89% sensitive and 99% specific for EAggEC identification. Thus, this probe should greatly facilitate epidemiologic studies assessing the importance of EAggEC as a diarrheal pathogen.read more
Citations
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Diarrheagenic Escherichia coli
James P. Nataro,James B. Kaper +1 more
TL;DR: The current level of understanding of the pathogenesis of the diarrheagenic E. coli strains is discussed and how their pathogenic schemes underlie the clinical manifestations, diagnostic approach, and epidemiologic investigation of these important pathogens are described.
Journal ArticleDOI
Recent Advances in Understanding Enteric Pathogenic Escherichia coli
Matthew A. Croxen,Robyn J. Law,Roland Scholz,Kristie M. Keeney,Marta Wlodarska,B. Brett Finlay +5 more
TL;DR: A comprehensive review highlights recent advances in understanding of the intestinal pathotypes of E. coli, which carry an enormous potential to cause disease and continue to present challenges to human health.
Journal ArticleDOI
Diarrheagenic Escherichia coli.
Tânia A. T. Gomes,Waldir P. Elias,Isabel C. A. Scaletsky,Beatriz E. C. Guth,Juliana Rodrigues,Roxane M. F. Piazza,Luís Carlos de Souza Ferreira,Marina Baquerizo Martinez +7 more
TL;DR: This review has gathered information on current definitions, serotypes, lineages, virulence mechanisms, epidemiology, and diagnosis of the major diarrheagenic E. coli pathotypes.
Journal ArticleDOI
Presence of adherent Escherichia coli strains in ileal mucosa of patients with Crohn's disease
Arlette Darfeuille-Michaud,Christel Neut,Nicolas Barnich,E Lederman,Patrick Di Martino,Pierre Desreumaux,Luc Gambiez,B Joly,Antoine Cortot,Jean-Frederic Colombel +9 more
TL;DR: E. coli strains isolated from the ileal mucosa of patients with Crohn's disease adhere to differentiated intestinal cells and may disrupt the intestinal barrier by synthesizing an alpha-hemolysin.
Journal ArticleDOI
Pathogenomics of the Virulence Plasmids of Escherichia coli
Timothy J. Johnson,Lisa K. Nolan +1 more
TL;DR: The evolution of these virulence plasmids and the implications of their acquisition by E. coli are now better understood and appreciated, with the available plasmid genomic sequences for several E. Escherichia coli pathotypes being compared in an effort to understand the evolution and define their core and accessory components.
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