A simple procedure for gel electrophoresis and Northern blotting of RNA
Sayed K. Goda,Nigel P. Minton +1 more
Reads0
Chats0
About:
This article is published in Nucleic Acids Research.The article was published on 1995-08-25 and is currently open access. It has received 132 citations till now. The article focuses on the topics: Far-western blotting & Southwestern blot.read more
Citations
More filters
Journal ArticleDOI
In vitro selection and evolution of functional proteins by using ribosome display.
Jozef Hanes,Andreas Plückthun +1 more
TL;DR: Libraries of native folded proteins can now be screened and made to evolve in a cell-free system without any transformation or constraints imposed by the host cell.
Journal ArticleDOI
Bleach Gel: A Simple Agarose Gel for Analyzing RNA Quality
TL;DR: The ‘bleach gel’ is a functional approach that addresses the need for an inexpensive and safe way to evaluate RNA integrity and will improve the ability of researchers to rapidly analyze RNA quality.
Journal ArticleDOI
Rifins: a second family of clonally variant proteins expressed on the surface of red cells infected with Plasmodium falciparum.
TL;DR: It is shown that a second gene family, rif, which is associated with var at subtelomeric sites in the genome, encodes clonally variant proteins (rifins) that are expressed on the infected red cell surface, indicating an important role for rifins in malaria host-parasite interaction.
Journal ArticleDOI
Use of the red fluorescent protein as a marker of Kaposi's sarcoma-associated herpesvirus lytic gene expression
TL;DR: RKSHV.219 efficiently established latent infections from which the virus could be reactivated to productive lytic replication and the production of infectious virus, and demonstrated strong synergy between KSHV/RTA and butyrate for the activation of KSHv lytic replicate and theproduction of infectious viruses.
References
More filters
Journal ArticleDOI
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
TL;DR: A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.
Journal ArticleDOI
RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
TL;DR: RNA molecular weight measurements were carried out by gel electrophoresis under four different denaturing conditions including 99% formamide, 10 mM methyl mercury, 2.2 M formaldehyde, and 6 M urea at pH 3.8 to demonstrate reliable molecular weight determinations of denatured RNAs, especially those obtained by extrapolation.
Journal ArticleDOI
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange
TL;DR: A simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis, using the metachromatic stain acridine orange for visualization ofucleic acids in gels and which can be analyzed on the same slab gel.
Journal ArticleDOI
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
James M. Bailey,Norman Davidson +1 more
TL;DR: It appears that complete denaturation of any base-paired secondary structural feature of a nucleic aicd can be achieved at practical concentrations of CH3HgOH.
Journal ArticleDOI
Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels
TL;DR: Poly A containing RNA isolated from embryonic chick calvaria was transferred from 6% formaldehyde 0.75% agarose gels to diazobenzyloxymethyl paper and the paper was hybridized to either nick translated pro alpha 1 collagen cDNA clones, pCg1 or pCG54, or to the nick translation pro alpha 2 collagen c DNA clone,pCg45.