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Nigel P. Minton
Researcher at University of Nottingham
Publications - 301
Citations - 15820
Nigel P. Minton is an academic researcher from University of Nottingham. The author has contributed to research in topics: Gene & Clostridium difficile. The author has an hindex of 62, co-authored 279 publications receiving 14005 citations. Previous affiliations of Nigel P. Minton include Salisbury University & Biotechnology and Biological Sciences Research Council.
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Journal ArticleDOI
The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome
Mohammed Sebaihia,Brendan W. Wren,Peter Mullany,Neil F. Fairweather,Nigel P. Minton,Richard A. Stabler,Nicholas R. Thomson,Adam P. Roberts,Ana Cerdeño-Tárraga,Hongmei Wang,Matthew T. G. Holden,Anne Wright,Carol Churcher,Michael A. Quail,Stephen Baker,Nathalie Bason,Karen Brooks,Tracey Chillingworth,Ann Cronin,Paul Davis,Linda Dowd,Audrey Fraser,Theresa Feltwell,Zahra Hance,S. Holroyd,Kay Jagels,Sharon Moule,Karen Mungall,Claire Price,Ester Rabbinowitsch,Sarah Sharp,Mark Simmonds,K. Stevens,Louise Unwin,Sally Whithead,Bruno Dupuy,Gordon Dougan,Bart Barrell,Julian Parkhill +38 more
TL;DR: The complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain, is determined; it indicates that a large proportion (11%) of the genome consists of mobile genetic elements, mainly in the form of conjugative transposons.
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The role of toxin A and toxin B in Clostridium difficile infection
Sarah A. Kuehne,Stephen T. Cartman,John T. Heap,Michelle L. Kelly,Alan Cockayne,Nigel P. Minton +5 more
TL;DR: It is shown that isogenic mutants of C. difficile producing either toxin A or toxin B alone can cause fulminant disease in the hamster model of infection, re-establish the importance of both toxin A and toxin B and highlight the need to continue to consider both toxins in the development of diagnostic tests and effective countermeasures against C. diffuse.
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The ClosTron: a universal gene knock-out system for the genus Clostridium.
TL;DR: A mutagenesis system based on the mobile group II intron from the ltrB gene of Lactococcus lactis to function in clostridial hosts is adapted, which is highly efficient and reproducible, and should revolutionize functional genomic studies inClostridia.
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A modular system for Clostridium shuttle plasmids.
TL;DR: The specification, design and construction of a standardized modular system for Clostridium-Escherichia coli shuttle plasmids was described, and existing replicons and selectable markers were incorporated, along with a novel clostridial replicon.
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The pMTL nic− cloning vectors. I: Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing
TL;DR: During the construction of these new vectors data were obtained which demonstrated that the pUC and pMTL plasmids contain a previously unreported single base pair difference within the RNA I/RNA II region responsible for a three-fold increase in plasmid copy number.