Journal ArticleDOI
Acute Self-Suppression of Corticosteroidogenesis in Isolated Adrenocortical Cells
Rocco V. Carsia,Sasha Malamed +1 more
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TLDR
Self-suppression of adrenocortical cells suggests the existence of a mechanism for the find adjustment of steroidogenesis that operates in addition to the classical control exerted by the anterior pituitary.Abstract:
The relation between steroidogenesis induced by ACTH and that induced by exogenous concentrations of glucocorticoids was studied in isolated adrenocortical cells. Exogenous corticosterone and cortisol, in concentrations within the production capacity of the adrenal gland, suppressed steroidogenesis induced by ACTH in rat and beef cells, respectively. The precursors pregnenolone and progesterone enhanced steroidogenesis in both rat and beef cells. Aldosterone in rat cells and 17 beta-estradiol in rat and beef cells had little if any effect on steroidogenesis. Either suppression or stimulation by exogenous steroids was acute, that is, after 2-h incubation for rat cells and 1-h incubation for beef cells. A direct suppressive action of end product glucocorticoids is indicated. This observed self-suppression of adrenocortical cells suggests the existence of a mechanism for the find adjustment of steroidogenesis that operates in addition to the classical control exerted by the anterior pituitary.read more
Citations
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Maturation and activation of hypothalamic-pituitary-adrenal function in fetal sheep
John R. G. Challis,A. N. Brooks +1 more
TL;DR: It is suggested that the progressive rise in the concentration of cortisol in the plasma of fetal sheep during late pregnancy arises from the sequential maturation of the fetal HPA axis and is broken by the process of birth.
Journal ArticleDOI
Intracellular movement of cholesterol in rat adrenal cells. Kinetics and effects of inhibitors.
J F Crivello,C R Jefcoate +1 more
Journal ArticleDOI
Role of glucocorticoid negative feedback in the regulation of HPA axis pulsatility
TL;DR: The knowledge of the negative feedback mechanisms that regulate basal ultradian synthesis and secretion of glucocorticoids, including the role of glucOCorticoid and mineralocortioid receptors and their chaperone protein FKBP51 are discussed.
Book ChapterDOI
Neurochemical Responses in Stress: Relationships Between the Hypothalamic-Pituitary-Adrenal and Catecholamine Systems
Adrian J. Dunn,Neal R. Kramarcy +1 more
TL;DR: The purpose of this chapter is to review some of the neurochemical responses that occur during stress and to assess their potential significance for the organism.
Journal ArticleDOI
Demonstration of Glucocorticoid Receptors in the Adrenal Cortex: Evidence for a Direct Dexamethasone Suppressive Effect on the Rat Adrenal Gland
TL;DR: The data indicate that the adrenal cortex possesses a high affinity binding site that fulfills the criteria for a glucocorticoid receptor and contributes to the suppressive effect of glucOCorticoids on the pituitary-adrenal axis.
References
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Journal ArticleDOI
Practical Procedure for Estimation of Corticosterone or Hydrocortisone
TL;DR: A simplified fluorometric procedure for corticosterone or hydrocortisone has been described and has practical applications for the estimation of these steroids in samples that are small in volume or low in concentration, or in studies that are designed primarily to demonstrate increases or decreases in adrenocortical hormones in plasma or adrenals without requiring absolute specificity.
Journal ArticleDOI
An Improved Technique for the Preparation of Isolated Rat Adrenal Cells: A Sensitive, Accurate and Specific Method for the Assay of ACTH
TL;DR: The accuracy of the isolated adrenal cell method for assay of ACTH is excellent and the cells are highly selective; they do not respond to relatively large quantities of insulin, glucagon, oxytocin, vasopressin or angiotensin II.
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Dispersed rat parotid acinar cells. I. Morphological and functional characterization.
TL;DR: Morphologically and functionally intact acinar cells obtained from the rat parotid gland through enzymatic dispersion with pure collagenase, hyaluronidase, and trypsin as well as mild mechanical forces could be used as experimental tools for in vitro studies of receptor physiology and biochemistry, cell membrane function, cellular secretory mechanisms, and other parameters of exocrine gland cell physiology.
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Interaction between plasma corticosterone concentration and adrenocorticotropinreleasing stimuli in the rat: evidence for the reset of an endocrine feedback control12
TL;DR: The hypothesis that the rapid rises in plasma corticosteroid concentration which follow many varied stimuli are caused by a reset of the negative feedback control of plasma cortingosterone concentration to regulate at higher levels is tested.
Journal ArticleDOI
A Role for Intraluteal Estrogen in the Mediation of Luteinizing Hormone Action on the Rat Corpus Luteum during Pregnancy
TL;DR: Treatment with LH antiserum plus a large testosterone implant prevented the decline in serum progesterone and nuclear Estradiol receptor and caused a dramatic elevation in luteal estradiol content.