Adenoviral-mediated transfer of TGF-β1 but not IGF-1 induces chondrogenic differentiation of human mesenchymal stem cells in pellet cultures
Koichiro Kawamura,Constance R. Chu,Satoshi Sobajima,Paul D. Robbins,Freddie H. Fu,Nicholas J. Izzo,Christopher Niyibizi +6 more
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TLDR
It is suggested that sustained administration of TGF-beta1 may be more effective in suppressing terminal differentiation than intermittent dosing and thus effective for cartilage repair.About:
This article is published in Experimental Hematology.The article was published on 2005-08-01 and is currently open access. It has received 102 citations till now. The article focuses on the topics: Transforming growth factor, beta 3 & Cellular differentiation.read more
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Growth factor gradients via microsphere delivery in biopolymer scaffolds for osteochondral tissue engineering
Xiaoqin Wang,Esther Wenk,Xiaohui Zhang,Lorenz Meinel,Lorenz Meinel,Gordana Vunjak-Novakovic,David L. Kaplan +6 more
TL;DR: A novel silk microsphere/scaffold system offers a new option for the delivery of multiple growth factors with spatial control in a 3D culture environment for both understanding natural tissue growth process and in vitro engineering complex tissue constructs.
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Controlled release of bioactive TGF-β1 from microspheres embedded within biodegradable hydrogels
TL;DR: Based on in vitro observations, it is predicted that a microsphere-loaded hydrogel for controlled release of TGF-beta1 to a cartilage wound site can be developed.
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Adeno-associated viral gene transfer of transforming growth factor-beta1 to human mesenchymal stem cells improves cartilage repair.
TL;DR: Results show that AAV is a suitable vector for gene delivery to improve the cartilage repair potential of human mesenchymal stem cells.
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Co-culture induces mesenchymal stem cell differentiation and modulation of the degenerate human nucleus pulposus cell phenotype
TL;DR: It is suggested that cellular interactions between MSCs and degenerate NP cells may both stimulate MSC differentiation to an NP-like phenotype and also stimulate the endogenous NP cell population to regain a nondegenerate phenotype and consequently enhance matrix synthesis for self-repair.
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Synovium-derived stem cell-based chondrogenesis.
TL;DR: A two-step protocol for the derivation of chondrogenic SDSCs is proposed: a cocktail of TGF-beta1, IGF-I, and FGF-2 is applied first to induce cell growth followed by a cocktail Ofsted-like growth factor I and IGF-2 applied to induce chONDrogenesis.
References
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Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Human Adipose Tissue Is a Source of Multipotent Stem Cells
Patricia A. Zuk,Min Zhu,Peter Ashjian,Daniel A. De Ugarte,Jerry I. Huang,Hiroshi Mizuno,Zeni Alfonso,John K. Fraser,Prosper Benhaim,Marc H. Hedrick +9 more
TL;DR: To confirm whether adipose tissue contains stem cells, the PLA population and multiple clonal isolates were analyzed using several molecular and biochemical approaches and PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression.
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Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation
TL;DR: Cultured autologous chondrocytes can be used to repair deep cartilage defects in the femorotibial articular surface of the knee joint.
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Bone marrow cells regenerate infarcted myocardium
Donald Orlic,Jan Kajstura,Stefano Chimenti,Igor Jakoniuk,Stacie M. Anderson,Baosheng Li,James Pickel,Ronald D.G. McKay,Bernardo Nadal-Ginard,David M. Bodine,Annarosa Leri,Piero Anversa +11 more
TL;DR: It is indicated that locally delivered bone marrow cells can generate de novo myocardium, ameliorating the outcome of coronary artery disease.
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Mesenchymal stem cells
TL;DR: The study of mesenchymal stem cells, whether isolated from embryos or adults, provides the basis for the emergence of a new therapeutic technology of self‐cell repair.