Journal ArticleDOI
Amperometric Biosensors for Detection of Sugars Based on the Electrical Wiring of Different Pyranose Oxidases and Pyranose Dehydrogenases with Osmium Redox Polymer on Graphite Electrodes
Federico Tasca,Suna Timur,Roland Ludwig,Dietmar Haltrich,Jindrich Volc,Riccarda Antiochia,Lo Gorton +6 more
Reads0
Chats0
TLDR
The biosensors, which showed the best characteristics in terms of linear range, detection limit and sensitivity were selected, viz. wild type P20 from T multicolor and PDH from A. meleagris were used and investigated for their selectivity for a number of different sugars.Abstract:
Electrical wiring of different types of pyranose oxidase (P20) (fungal wild type, recombinant wild type with a hexahistidine tag, mutant form E542K with a hexa-histidine tag) from Trametes multicolor, and recombinant P20 from Coriolus sp overexpressed in Escherichia coli as well as of pyranose dehydrogenase (PDH) from Agaricus meleagris and Agaricus xanthoderma with an osmium redox polymer (poly(1-vinylimidazole)(12)-[Os(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl2+/+) on graphite electrodes was carried out After optimization studies using glucose as substrate, the biosensors, which showed the best characteristics in terms of linear range, detection limit and sensitivity were selected, viz wild type P20 from T multicolor and PDH from A meleagris These two enzymes were used and investigated for their selectivity for a number of different sugars (Less)read more
Citations
More filters
Journal ArticleDOI
Cellobiose Dehydrogenase: A Versatile Catalyst for Electrochemical Applications.
TL;DR: The application of electrochemical techniques to the elucidation of the molecular and catalytic properties of cellobiose dehydrogenase is discussed and compared to biochemical methods, which lead to valuable insights into the function of this cellulose-bound enzyme and form the basis of exciting applications in biosensors, biofuel cells and bioelectrocatalysis.
Journal ArticleDOI
Cellobiose dehydrogenase aryl diazonium modified single walled carbon nanotubes: Enhanced direct electron transfer through a positively charged surface
TL;DR: Very efficient direct electron transfer (DET) between cellobiose dehydrogenase (CDH) from Phanerochaete sordida and surface modified single walled carbon nanotubes (SWCNT) is reported on, which potentially reduces the interference by oxidizable matrix components in biosensors and increases the open circuit potential in biofuel cells.
Journal ArticleDOI
Characterization of different FAD-dependent glucose dehydrogenases for possible use in glucose-based biosensors and biofuel cells
TL;DR: Different flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenases were characterized electrochemically after “wiring” them with an osmium redox polymer on graphite electrodes to see whether the reduced glycosylation would have an effect, e.g., a higher current density, which was indeed found.
Journal ArticleDOI
Electrochemical glucose biosensing by pyranose oxidase immobilized in gold nanoparticle-polyaniline/AgCl/gelatin nanocomposite matrix
TL;DR: In this paper, a novel pyranose oxidase (PyOx) biosensor based on gold nanoparticles (AuNPs)-polyaniline(PANI)/AgCl/gelatin nanocomposite has been developed for the glucose detection.
Journal ArticleDOI
Direct Electron Transfer at Cellobiose Dehydrogenase Modified Anodes for Biofuel Cells
TL;DR: The highest electrocatalytic activity toward oxidation of lactose was found for CDH from the basidiomycete Phanerochaete sordida, and an anode for biofuel cells was developed, using this anode using lactose as substrate as a model for a membrane-less biofuel cell.
References
More filters
Journal ArticleDOI
Enzymatic biofuel cells for implantable and microscale devices.
TL;DR: This review paper summarizes the current state of enzymatic biofuel cell research in the context of foreseeable applications and assesses the future prospects of the technology.
Journal ArticleDOI
Biofuel cells and their development
TL;DR: While considerable chemical development of enzyme electrodes has occurred, relatively little progress has been made towards the engineering development of biofuel cells.
Journal ArticleDOI
Electrical Connection of Enzyme Redox Centers to Electrodes
TL;DR: In this paper, the self-exchange rate of the relays and their density are high, and the flux of electrons through a 1 micrometer thick film of a 3-dimensional macromolecular network can match or exceed the rate of supply of electrons to or from the ensemble of enzyme molecules covalently bound to it.
Journal ArticleDOI
Characteristics of a Miniature Compartment-less Glucose-O2 Biofuel Cell and Its Operation in a Living Plant
Nicolas Mano,Fei Mao,Adam Heller +2 more
TL;DR: The temperature, pH, glucose concentration, NaCl concentration, and operating atmosphere dependence of the power output of a compartment-less miniature glucose-O(2) biofuel cell, comprised only of two bioelectrocatalyst-coated carbon fibers, is reported.
Journal ArticleDOI
Enzymatic determination of glucose in a flow system by catalytic oxidation of the nicotinamide coenzyme at a modified electrode
TL;DR: In this article, a chemically modified electrode for detection of dihydronicotinamide adenine dinucleotide (NADH) and dihydronamide adnine din nucleotide phosphate was described, where graphite rods were modified by dipping them into solutions ofdimethylamino-1,2-benzophenoxzinium salt.