Journal ArticleDOI
An autonomous T-rich DNA machine based lateral flow biosensor for amplified visual detection of mercury ions
Jie Liu,Jie Liu,Lingbo Chen,Lingbo Chen,Junhua Chen,Chenchen Ge,Zhiyuan Fang,Lin Wang,Xuerong Xing,Xuerong Xing,Lingwen Zeng +10 more
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TLDR
An autonomous thymine rich DNA machine as an amplification unit was developed for the sensitive detection of mercury ions with high specificity and can be read out by the naked eye with a detection limit of 5 nM.Abstract:
An autonomous thymine rich DNA machine as an amplification unit was developed for the sensitive detection of mercury ions with high specificity. Combined with a lateral flow biosensor, the amplified signal of Hg2+ can be read out by the naked eye with a detection limit of 5 nM.read more
Citations
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Nucleic Acid Biosensors: Recent Advances and Perspectives
Yan Du,Shaojun Dong +1 more
Journal ArticleDOI
Target-fueled DNA walker for highly selective miRNA detection
TL;DR: A DNA walking biosensor that can realize the detection of let-7a with a detection limit of 58 fM and high selectivity for resolving one nucleotide variation is reported.
Journal ArticleDOI
Development of mercury (II) ion biosensors based on mercury-specific oligonucleotide probes
TL;DR: Recently, various novel Hg(2+) biosensors have been developed based on T-rich Mercury-Specific Oligonucleotide (MSO) probes, and exhibited advanced selectivity and excellent sensitivity for Hg (2+) detection.
Journal ArticleDOI
One-step competitive lateral flow biosensor running on an independent quantification system for smart phones based in-situ detection of trace Hg(II) in tap water.
TL;DR: The approach provides a simple, sensitive, and practical tool for the detection of trace Hg(II) in tap water, showing great promise for in-situ applications.
Journal ArticleDOI
ExoIII and TdT dependent isothermal amplification (ETDA) colorimetric biosensor for ultra-sensitive detection of Hg2.
TL;DR: Combined with the colorimetric biosensor and double enzyme cyclic amplification reaction, an ultra-sensitivity and strong specificity detection method was developed to detect Hg2+ and expands the detection method of Hg 2+ available in the literature.
References
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Journal ArticleDOI
Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.
TL;DR: An isothermal in vitro DNA amplification method was developed based upon the following sequence of reaction events: Restriction enzyme cleavage and subsequent heat denaturation of a DNA sample generates two single-stranded target DNA fragments (T1 and T2).
Journal ArticleDOI
Optical Analysis of Hg2+ Ions by Oligonucleotide–Gold-Nanoparticle Hybrids and DNA-Based Machines
TL;DR: Zwei verschiedene optische Methoden fur die Analyse von Hg2+-Ionen, die auf der Bildung von Thymin-Hg2-Komplexen beruhen, werden vorgestellt.
Journal ArticleDOI
15N−15N J-Coupling Across HgII: Direct Observation of HgII-Mediated T−T Base Pairs in a DNA Duplex
TL;DR: N−N J-coupling across a metal center (2JNN) was clearly detected in a biological macromolecule (DNA duplex) for the first time and the base pairing mode of mercury-mediated T−T pairs (T−HgII−T) was definitely determined.
Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system (nucleic acid amplification/strand displacement/Mycobacterium tuberculosis)
TL;DR: An isothermal in vitro DNA amplification method was developed based upon the following sequence of reaction events: restriction enzyme cleavage and subsequent heat denaturation of a DNA sample generates two single-stranded target DNA fragments (T1 and T2), present in excess are two DNA amplification primers (P1 and P2).
Journal ArticleDOI
Gold nanoparticle probes for the detection of mercury, lead and copper ions.
TL;DR: The roles of several factors affecting the selectivity and sensitivity of the nanosensors toward the analytes are discussed: the size of the Au nanomaterial, the length and sequence of the DNA or the nature of the thiol, the surface density of the recognition ligand, the ionic strength and pH of the buffer solution.
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