Analysis of Flavone C-Glycosides in the Leaves of Clinacanthus nutans (Burm. f.) Lindau by HPTLC and HPLC-UV/DAD
June Lee Chelyn,Maizatul Hasyima Omar,Nor Syaidatul Akmal Mohd Yousof,Ramesh Ranggasamy,Mohd Isa Wasiman,Zakiah Ismail +5 more
TLDR
A two-step method using thin-layer Chromatography (TLC) and high pressure liquid chromatography (HPLC) for the rapid identification and quantification of the flavones C-glycosides in C. nutans leaves is developed.Abstract:
Clinacanthus nutans (family Acanthaceae) has been used for the treatment of inflammation and herpes viral infection. Currently, there has not been any report on the qualitative and quantitative determination of the chemical markers in the leaves of C. nutans. The C-glycosidic flavones such as shaftoside, isoorientin, orientin, isovitexin, and vitexin have been found to be major flavonoids in the leaves of this plant. Therefore, we had developed a two-step method using thin-layer chromatography (TLC) and high pressure liquid chromatography (HPLC) for the rapid identification and quantification of the flavones C-glycosides in C. nutans leaves. The TLC separation of the chemical markers was achieved on silica gel 60 plate using ethyl acetate : formic acid : acetic acid : water (100 : 11 : 11 : 27 v/v/v/v) as the mobile phase. HPLC method was optimized and validated for the quantification of shaftoside, orientin, isovitexin, and vitexin and was shown to be linear in concentration range tested (0.4–200 μg/mL, r2 ≥ 0.996), precise (RSD ≤ 4.54%), and accurate (95–105%). The concentration of shaftoside, orientin, vitexin, and isovitexin in C. nutans leave samples was 2.55–17.43, 0.00–0.86, 0.00–2.01, and 0.00–0.91 mmol/g, respectively.read more
Citations
More filters
Journal ArticleDOI
Effects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti-radical activity of Clinacanthus nutans Lindau leaves by response surface methodology.
Intan Soraya Che Sulaiman,Mahiran Basri,Hamid Reza Fard Masoumi,Wei Jian Chee,Siti Efliza Ashari,Maznah Ismail +5 more
TL;DR: The quadratic polynomial models developed were satisfactorily accurate to be used in analyzing the interactions of the parameters (response and independent variables) and could be useful in the development of cosmeceutical products containing extracts of C. nutans.
Journal ArticleDOI
Clinacanthus nutans: a review on ethnomedicinal uses, chemical constituents and pharmacological properties.
TL;DR: This article compiles the ethnomedicinal uses of CN and its phytochemistry, and thus provides a phytochemical library of CN, and discusses the known pharmacological and biological effects of CN to enable better investigation of CN.
Journal ArticleDOI
Clinacanthus nutans (Burm. f.) Lindau Ethanol Extract Inhibits Hepatoma in Mice through Upregulation of the Immune Response
TL;DR: It is indicated that CN30 demonstrated antitumor properties by up-regulating the immune response, and warrants further evaluation as a potential therapeutic agent for the treatment and prevention of cancers.
Journal ArticleDOI
A Comprehensive Review on Phytochemistry and Pharmacological Activities of Clinacanthus nutans (Burm.f.) Lindau.
Leng Wei Khoo,Siew Foong Audrey Kow,Ming Tatt Lee,Chin Ping Tan,Khozirah Shaari,Chau Ling Tham,Faridah Abas +6 more
TL;DR: Pharmacological studies on C. nutans revealed that its polar extract could be a promising anti-inflammation, antiviral, anticancer, immune and neuromodulating, and plasmid DNA protective agent; and that its nonpolar Extract could be an excellent anticancer agent.
Journal ArticleDOI
Phytosterols isolated from Clinacanthus nutans induce immunosuppressive activity in murine cells
Cheng Foh Le,Thina Hareesh Kailaivasan,Sek-Chuen Chow,Zunoliza Abdullah,Sui-Kiong Ling,Chee-Mun Fang +5 more
TL;DR: Results suggested that phytosterols isolated from C. nutans possess immunomodulatory effects with potential development as immunotherapeutic agents.
References
More filters
Book
A dictionary of the economic products of the Malay Peninsula
TL;DR: A Dictionary of the Economic Products of the Malay Peninsula as discussed by the authors, a dictionary of the economic products of theMalay Peninsula, a Dictionary of economic products in Malay, and a Malay economic dictionary.
Journal ArticleDOI
Extraction, separation, and detection methods for phenolic acids and flavonoids
TL;DR: This review provides an updated and extensive overview of methods and their applications in natural product matrices and samples of biological origin and critically appraises recent developments and trends.
Journal ArticleDOI
International conference on harmonization of technical requirements for registration of pharmaceuticals for human use.
Journal ArticleDOI
Isolation and purification of four flavone C-glycosides from antioxidant of bamboo leaves by macroporous resin column chromatography and preparative high-performance liquid chromatography
TL;DR: In this paper, a robust method for the preparation of four flavone C-glycosides, i.e. orientin, homoorientin, vitexin and isovitexins, which were prepared from an ethanol aqueous extract, was investigated.
Journal ArticleDOI
Bioactive constituents from the leaves of Clinacanthus nutans Lindau
Santi Sakdarat,Aussavashai Shuyprom,Chamsai Pientong,Tipaya Ekalaksananan,Sasithorn Thongchai +4 more
TL;DR: Three chlorophyll derivatives (phaeophytins) were isolated from the chloroform extract of Clinacanthus nutans Lindau leaves by means of chromatographic techniques and bioactivity-guided fractionation to give three pure compounds showing anti-herpes simplex activity and inhibitory activity affected the virus before viral entry to the host cells.