Journal ArticleDOI
Application of binary immobilized Candida rugosa lipase for hydrolysis of soybean oil
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TLDR
In this paper, a chitosan bead was used to immobilize a lipase and its catalytic efficiency in the hydrolysis of soybean oil was investigated, and the effects of temperature, pH, pH and oil to water ratio on the conversion and thermal stability, reusability, storage stability and kinetic properties were also investigated.Abstract:
Lipase was immobilized to chitosan beads by a binary method and its catalytic efficiency in the hydrolysis of soybean oil was investigated. In the first step, the hydroxyl groups of chitosan were activated with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and lipase molecules were coupled to the active hydroxyl groups. In the second step, more lipase molecules were cross-linked through its amino groups to chitosan by using glutaraldehyde. The effects of temperature, pH and oil to water ratio on the conversion, pH and thermal stability, reusability, storage stability and the kinetic properties were also investigated. Under optimal conditions, 88% of the oil taken initially was hydrolyzed after 5 h. Better thermal stability was exhibited by the immobilized lipase and the pH stability was comparable to that of soluble lipase. Storage for 30 days at 4 °C, showed that the immobilized enzyme did not lose its activity. The relative activity upon six repeated uses was 80%.read more
Citations
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Journal ArticleDOI
Immobilized lipase on magnetic chitosan microspheres for transesterification of soybean oil
Wenlei Xie,Jianlong Wang +1 more
TL;DR: In this article, magnetic chitosan microspheres were prepared by the chemical co-precipitation approach using glutaraldehyde as cross-linking reagent for lipase immobilization.
Journal ArticleDOI
Immobilization of lipase on hydrophobic nano-sized magnetite particles
Dong Geun Lee,Kanagasabai Muruganandam Ponvel,Mir Kim,Sangpill Hwang,Ik Sung Ahn,Chang Ha Lee +5 more
TL;DR: In this article, surface modified nano-sized magnetite (S-NSM) particles have been suggested as a support for the immobilization of enzyme in a study based on the finding that a lipase is strongly adsorbed onto a hydrophobic surface.
Journal ArticleDOI
Electrospun polyacrylonitrile nanofibrous membranes for lipase immobilization
TL;DR: This lipase immobilization method shows the best performance among various immobilized lipase systems using the same source of lipase and substrate when considering protein loading, activity retention, and kinetic parameters.
Journal ArticleDOI
Enzymatic reactors for biodiesel synthesis: Present status and future prospects
TL;DR: This review has analyzed the main factors involved in the enzymatic synthesis of biodiesel, focusing in the choice of the immobilization protocol, and the parameters involved inThe choice and configuration of the reactors.
Journal ArticleDOI
Preparation Fe3O4@chitosan magnetic particles for covalent immobilization of lipase from Thermomyces lanuginosus.
TL;DR: The results showed that the immobilization of lipase onto magnetic chitosan nanoparticles by the method of CCEE is an efficient and simple way for preparation of stable lipase.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Production, purification, characterization, and applications of lipases.
TL;DR: The production, recovery, and use of microbial lipases are discussed; issues of enzyme kinetics, thermostability, and bioactivity are addressed; and production of recombinant lipases is detailed.
Journal ArticleDOI
The realm of microbial lipases in biotechnology
Ashok Pandey,Sailas Benjamin,Carlos Ricardo Soccol,Poonam Singh Nee Nigam,Nadia Krieger,Vanete Thomaz Soccol +5 more
TL;DR: Widening applications such as those in waste management and improved tanning techniques are other novel aspects of lipase utilization that are discussed in this review.
Journal ArticleDOI
Customizing lipases for biocatalysis: a survey of chemical, physical and molecular biological approaches
TL;DR: The present review will survey the different procedures that have been developed to enhance the properties of lipases by physical modifications of the biocatalysts by adsorption on a carrier material, entrapment or microencapsulation.
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