Journal ArticleDOI
Application of the micropipette technique to the measurement of cultured porcine aortic endothelial cell viscoelastic properties.
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TLDR
The actin microfilaments were found to be the major cytoskeletal component determining the viscoelastic response of endothelial cells grown in static culture.Abstract:
The viscoelastic deformation of porcine aortic endothelial cells grown under static culture conditions was measured using the micropipette technique. Experiments were conducted both for control cells (mechanically or trypsin detached from the substrate) and for cells in which cytoskeletal elements were disrupted by cytochalasin B or colchicine. The time course of the aspirated length into the pipette was measured after applying a stepwise increase in aspiration pressure. To analyze the data, a standard linear viscoelastic half-space model of the endothelial cell was used. The aspirated length was expressed as an exponential function of time. The actin microfilaments were found to be the major cytoskeletal component determining the viscoelastic response of endothelial cells grown in static culture.read more
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Micropipette aspiration of living cells.
TL;DR: The mechanical behavior of living cells is studied with micropipette suction in which the surface of a cell is aspirated into a small glass tube while tracking the leading edge of its surface to get values for a cell's elastic and viscous properties.
Journal ArticleDOI
Emergent patterns of growth controlled by multicellular form and mechanics.
Celeste M. Nelson,Ronald P. Jean,John L. Tan,Wendy Liu,Nathan J. Sniadecki,Alexander A. Spector,Christopher S. Chen +6 more
TL;DR: The existence of patterns of mechanical forces that originate from the contraction of cells, emerge from their multicellular organization, and result in patterns of growth are demonstrated, demonstrating that tissue form itself can feed back to regulate patterns of proliferation.
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Mechanical models for living cells--a review
TL;DR: This review of mechanical models developed to characterize mechanical responses of living cells when subjected to both transient and dynamic loads finds that the biphasic model has been widely used to study musculoskeletal cell mechanics.
Journal ArticleDOI
Cell prestress. I. Stiffness and prestress are closely associated in adherent contractile cells
Ning Wang,Iva Marija Tolić-Norrelykke,Jianxin Chen,Srboljub M. Mijailovich,James P. Butler,Jeffrey J. Fredberg,Dimitrije Stamenović +6 more
TL;DR: Data establish a strong association between stiffness of HASM cells and the level of tensile stress within the cytoskeleton, a hallmark of systems that secure shape stability mainly through the prestress.