Applications of Holographic Microscopy in Life Sciences
Iliyan Peruhov,Emilia Mihaylova +1 more
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TLDR
The digital holographic microscopy is a new imaging technology applied to optical microscopy that yields a 3D volume image from a single image capture.Abstract:
Imaging of microscopic objects is an essential art, especially in life sciences Rapid progress in electronic detection and control, digital imaging, image processing, and numerical computa‐ tion has been crucial in advancing modern microscopy At present the 3D imaging of biological samples is done by confocal microscopes Their ability to image biological events in real time is limited by the time necessary to capture stacks of images taken through a certain plane in cells or tissues from which a 3D view is calculated Digital holographic microscopy is a new imaging technology applied to optical microscopy The digital holographic microscopy is a very advanced imaging technique because it yields a 3D volume image from a single image captureread more
Citations
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Proceedings ArticleDOI
Development and application of laser hologram production techniques for the teaching of physics and the public awareness of science
Proceedings ArticleDOI
Implementation of Kirchhoff-Helmholtz transform on GPU for use in digital in-line holographic microscopy
TL;DR: This work explores the evaluation of KHT using GPU at various levels of optimisation, exploiting the symmetries inherent in KHT and optimizations that are specific to the GPU architecture like the lookup tables, scheduling of read/writes.
Journal ArticleDOI
Recent Advances in the Application of Digital Holographic Microscopy
TL;DR: Digital holographic microscopy (DIHM) as discussed by the authors is a technique by which a wavefront can be recorded and subsequently reconstructed in the absence of the original wavefront, by replacing photochemical procedures with electronic recording of a hologram and its digital reconstruction, a wide range of new imaging capabilities become available.
References
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Journal ArticleDOI
Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy.
Pierre Marquet,Benjamin Rappaz,Pierre J. Magistretti,Etienne Cuche,Yves Emery,Tristan Colomb,Christian Depeursinge +6 more
TL;DR: A digital holographic microscope, in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells, is developed, based on an original numerical algorithm presented in detail elsewhere.
Book
Holographic and Speckle Interferometry: A Discussion of the Theory, Practice and Application of the Techniques
Robert Jones,Catherine Wykes +1 more
TL;DR: In this article, the authors present a technique for shape measurements using holographic and speckle pattern interferometry techniques, based on the concept of correlation interferometrics.
Journal ArticleDOI
Digital holographic microscopy for live cell applications and technical inspection
Björn Kemper,Gert von Bally +1 more
TL;DR: Digital holographic microscopy enables a quantitative phase contrast metrology that is suitable for the investigation of reflective surfaces as well as for the marker-free analysis of living cells.
Journal ArticleDOI
Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy
Benjamin Rappaz,Pierre Marquet,Etienne Cuche,Yves Emery,Christian Depeursinge,Pierre J. Magistretti +5 more
TL;DR: A digital holographic microscope adapted to the quantitative study of cellular dynamics, in a transmission mode, and allows to determine independently the thickness and the integral refractive index of cells.
Journal ArticleDOI
Digital in-line holographic microscopy.
Jorge Garcia-Sucerquia,W. Xu,Stephan K. Jericho,P. Klages,Manfred H. Jericho,H. Jürgen Kreuzer +5 more
TL;DR: The state of the art of digital in-line holographic microscopy with numerical reconstruction is reviewed and some technical issues, such as lateral and depth resolution, depth of field, twin image, four-dimensional tracking, and reconstruction algorithm are discussed.