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ARTICLE; AGRICULTURE AND ENVIRONMENTAL BIOTECHNOLOGY Bacillus safensis LAU 13: a new source of keratinase and its multi-functional biocatalytic applications
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TLDR
The obtained results showed an improvement in the properties of the mutant strain for use of the micro-organism or its enzyme as biocatalysts and high substrate specificity for feathers, but low specificity for human and bovine hairs.Abstract:
A newly isolated bacterium identified as Bacillus safensis based on biochemical tests and 16S rRNA analysis and its mutant variant created by exposure to ultraviolet radiation at 254 nm were investigated for keratinolytic activity. The wild-type strain produced 35.450.4 U/mL keratinase over a period of 120 h, while the mutant one yielded 64.4108.5 U/mL keratinase for the same period of 120 h. The optimal conditions for the enzyme activities were pH 7.5 and 40 C. The mutant and wild-type strain keratinases retained 59% and 54% of their activity after 12 h pretreatment at 40 C, and 64% and 60% of their activity after 12 h at pH 7.5, respectively. The keratinases showed high substrate specificity for feathers, but low specificity for human and bovine hairs. The enzymes were activated by Na C ,C a 2C ,F e 2C and Mg 2C . However, while Mn 2C activated the enzyme from the mutant strain, it inhibited that of the wild type. The mutant and wild-type strain completely degraded whole chicken feathers after 6 and 9 days at 30 § 2 C, and also completely dehaired goat skin within 12 and 16 h, respectively, without damage to the skin. Similarly, remarkable destaining of blood-stained cloth occurred within 23 h. The obtained results showed an improvement in the properties of the mutant strain for use of the microorganism or its enzyme as biocatalysts.read more
Citations
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Keratin: dissolution, extraction and biomedical application
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I. A. Adelere,Agbaje Lateef +1 more
TL;DR: A review article on the green synthesis of metallic nanoparticles using diverse agro-wastes, enzymes, and pigments of biological origin is presented in this article, which brings to the fore the emerging importance of these bio-resources for nanobiotechnological applications.
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Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13
Agbaje Lateef,I. A. Adelere,I. A. Adelere,Evariste B. Gueguim-Kana,Tesleem B. Asafa,Lorika S. Beukes +5 more
TL;DR: In this paper, a crude extracellular keratinase obtained from a novel keratin-degrading bacterial strain, Bacillus safensis LAU 13 (GenBank accession No. KJ461434) was used for the synthesis of silver nanoparti-cles (AgNPs).
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Biomedical and Catalytic Applications of Gold and Silver-Gold Alloy Nanoparticles Biosynthesized Using Cell-Free Extract of Bacillus Safensis LAU 13: Antifungal, Dye Degradation, Anti-Coagulant and Thrombolytic Activities
Sunday A. Ojo,Agbaje Lateef,Musibau A. Azeez,Suliat M. Oladejo,Abiola S. Akinwale,Tesleem B. Asafa,Taofeek A. Yekeen,Akeem Akinboro,Iyabo C. Oladipo,Evariste B. Gueguim-Kana,Lorika S. Beukes +10 more
TL;DR: The nanoparticles prevented coagulation of blood, and also completely dissolved blood clots, indicating the biomedical potential of AuNPs and Ag-AuNPs in the management of blood coagulated disorders.
References
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Journal ArticleDOI
Characterization of a protease of a feather‐degrading Microbacterium species
TL;DR: Aims: To characterize a new feather‐degrading bacterium.
Journal ArticleDOI
Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13
Agbaje Lateef,I. A. Adelere,I. A. Adelere,Evariste B. Gueguim-Kana,Tesleem B. Asafa,Lorika S. Beukes +5 more
TL;DR: In this paper, a crude extracellular keratinase obtained from a novel keratin-degrading bacterial strain, Bacillus safensis LAU 13 (GenBank accession No. KJ461434) was used for the synthesis of silver nanoparti-cles (AgNPs).
Journal ArticleDOI
Optimization of medium composition for keratinase production on feather by Bacillus licheniformis RG1 using statistical methods involving response surface methodology
Priya Ramnani,Rani Gupta +1 more
TL;DR: Keratinase production was found to be a function of biomass and maximum production occurred during the stationary phase, and further interaction of these factors, along with phosphate and incubation time, was studied using response surface methodology.
Journal ArticleDOI
Isolation, identification and characterization of a feather degrading bacterium.
TL;DR: Keratinases which are produced by these keratinolytic organisms could be used to degrade feather waste and further the digested products could be an excellent material for producing animal feed, fertilizers or natural gas.
Journal ArticleDOI
Water stress amelioration and plant growth promotion in wheat plants by osmotic stress tolerant bacteria
TL;DR: Results clearly indicate that the ability of wheat plants to withstand water stress is enhanced by application of these bacteria which also function as plant growth promoting rhizobacteria.