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ARTICLE; AGRICULTURE AND ENVIRONMENTAL BIOTECHNOLOGY Bacillus safensis LAU 13: a new source of keratinase and its multi-functional biocatalytic applications
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TLDR
The obtained results showed an improvement in the properties of the mutant strain for use of the micro-organism or its enzyme as biocatalysts and high substrate specificity for feathers, but low specificity for human and bovine hairs.Abstract:
A newly isolated bacterium identified as Bacillus safensis based on biochemical tests and 16S rRNA analysis and its mutant variant created by exposure to ultraviolet radiation at 254 nm were investigated for keratinolytic activity. The wild-type strain produced 35.450.4 U/mL keratinase over a period of 120 h, while the mutant one yielded 64.4108.5 U/mL keratinase for the same period of 120 h. The optimal conditions for the enzyme activities were pH 7.5 and 40 C. The mutant and wild-type strain keratinases retained 59% and 54% of their activity after 12 h pretreatment at 40 C, and 64% and 60% of their activity after 12 h at pH 7.5, respectively. The keratinases showed high substrate specificity for feathers, but low specificity for human and bovine hairs. The enzymes were activated by Na C ,C a 2C ,F e 2C and Mg 2C . However, while Mn 2C activated the enzyme from the mutant strain, it inhibited that of the wild type. The mutant and wild-type strain completely degraded whole chicken feathers after 6 and 9 days at 30 § 2 C, and also completely dehaired goat skin within 12 and 16 h, respectively, without damage to the skin. Similarly, remarkable destaining of blood-stained cloth occurred within 23 h. The obtained results showed an improvement in the properties of the mutant strain for use of the microorganism or its enzyme as biocatalysts.read more
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Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13
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Biomedical and Catalytic Applications of Gold and Silver-Gold Alloy Nanoparticles Biosynthesized Using Cell-Free Extract of Bacillus Safensis LAU 13: Antifungal, Dye Degradation, Anti-Coagulant and Thrombolytic Activities
Sunday A. Ojo,Agbaje Lateef,Musibau A. Azeez,Suliat M. Oladejo,Abiola S. Akinwale,Tesleem B. Asafa,Taofeek A. Yekeen,Akeem Akinboro,Iyabo C. Oladipo,Evariste B. Gueguim-Kana,Lorika S. Beukes +10 more
TL;DR: The nanoparticles prevented coagulation of blood, and also completely dissolved blood clots, indicating the biomedical potential of AuNPs and Ag-AuNPs in the management of blood coagulated disorders.
References
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Journal ArticleDOI
Keratinase. ii. properties of the crystalline enzyme.
TL;DR: Keratinase conjugate elaborated by Streptomyces fradiae has been resolved into a basic protein and a novel acidic polymer and was shown to be homogeneous by electrophoretic and ultracentrifugal analysis.
Journal Article
Biochemical and Molecular Characterization of Microbial Keratinase and Its Remarkable Applications
TL;DR: The bioconversion of insoluble feather keratin to soluble feather residue has high nutritional values and can be employed as a supplement for livestock feeds and other promising applications have been associated with keratinolytic enzymes.
Journal ArticleDOI
Genome Sequence of Salt-Tolerant Bacillus safensis Strain VK, Isolated from Saline Desert Area of Gujarat, India
V. V. Kothari,Ramesh K. Kothari,C. R. Kothari,Vaibhav D. Bhatt,Neelam M. Nathani,Prakash G. Koringa,Chaitanya G. Joshi,B. R. M. Vyas +7 more
TL;DR: The 3.68-Mb draft genome sequence of B. safensis VK is provided, which might provide information about the salt tolerance and genes encoding enzymes for the strain's plant growth-promoting potential.
Journal ArticleDOI
Application of a statistically enhanced, novel, organic solvent stable lipase from Bacillus safensis DVL-43
TL;DR: The molecular identification of a newly isolated bacterial strain producing a novel and organic solvent stable lipase, statistical optimization of fermentation medium, and its application in the synthesis of ethyl laurate are presented.
Journal ArticleDOI
Selection of halophilic bacteria for biological control of tomato gray mould caused by Botrytis cinerea
TL;DR: In vivo screening of the isolates, Bacillus safensis and Bacillus oceanisediminis CCMM B584 showed permanent antagonistic activity on tomato fruits, with 100% inhibition of B. cinerea after 7 days, suggesting these two strains may offer potential for biological control of tomato gray mould.