Journal ArticleDOI
Assessment of in vitro genotoxic and cytotoxic effects of flurbiprofen on human cultured lymphocytes.
Taygun Timocin,Hasan Basri Ila,Tuba C. Dördü,Mehmet Tahir Husunet,Mostafa Norizadeh Tazehkand,Ebrahim Valipour,Mehmet Topaktaş +6 more
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TLDR
The findings of this research showed that flurbiprofen had cytotoxic effects in human blood lymphocytes.Abstract:
Flurbiprofen is non-steroidal anti-inflammatory drug which is commonly used for its analgesic, antipyretic, and anti-inflammatory effects. The purpose of the study was to explore the genotoxic and cytotoxic effects of flurbiprofen in human cultured lymphocytes by sister chromatid exchange, chromosome aberration, and cytokinesis-blocked micronucleus tests. 10, 20, 30, and 40 μg/mL concentrations of flurbiprofen (solvent is DMSO) were used to treatment of human cultured lymphocytes at two different treatment periods (24 and 48 h). Flurbiprofen had no significant genotoxic effect in any of these tests. But exposing to flurbiprofen for 24 and 48 h led to significant decrease on proliferation index, mitotic index, and nuclear division index (NDI). Also, all decreases were concentration-dependent (except NDI at 24 h treatment period). Consequently, the findings of this research showed that flurbiprofen had cytotoxic effects in human blood lymphocytes.read more
Citations
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The role of natural indigo dye in alleviation of genotoxicity of sodium dithionite as a reducing agent.
TL;DR: The present study was designed to obtain the high yield of natural indigo dye but with low possible toxic effect, and aqueous extracts of Isatis leaves removed the toxic effects of sodium dithionite and showed anti-genotoxic effect.
Journal ArticleDOI
Genotoxic effects of 4-methylimidazole on human peripheral lymphocytes in vitro
Rima Çelik,Mehmet Topaktaş +1 more
TL;DR: The results showed that 4-MEI pose a genotoxic and cytotoxic effects for human peripheral lymphocytes.
Journal ArticleDOI
Evaluation of in vitro and in vivo genotoxic and antigenotoxic effects of Rhus coriaria.
TL;DR: The results showed that RCE possess cytotoxic effect in short-term treatments in vitro, however, it does not demonstrate genotoxic or cytotoxicity effects in vivo.
Journal ArticleDOI
In vitro potential cytogenetic and oxidative stress effects of roxithromycin.
TL;DR: Macrolide antibiotic roxithromycin did not have genotoxic and oxidative stress effects in human-cultured lymphocytes as well as in chromosome aberration and micronucleus tests.
Journal ArticleDOI
Assessment of the cytotoxic, genotoxic, and apoptotic potential of flurbiprofen in HeLa and HepG2 cell lines.
TL;DR: In this paper, the cytotoxicity, genotoxic, and apoptotic effects of flurbiprofen were evaluated in human cervical and liver cancer cells, and it was observed that cytoxoxicity increased in a concentration-and time-dependent manner.
References
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The in vitro micronucleus technique.
TL;DR: In its current basic form the CBMN assay can provide the following measures of genotoxicity and cytotoxicity: chromosome breakage, chromosome loss, chromosome rearrangement, cell division inhibition, necrosis and apoptosis.
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IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans
Richard J. Albertini,Diana Anderson,George R. Douglas,Lars Hagmar,Kari Hemminki,Franco Merlo,Adayapalam T. Natarajan,Hannu Norppa,David E. G. Shuker,Raymond R. Tice,Michael D. Waters,Antero Aitio +11 more
TL;DR: The most commonly studied genotoxicity endpoints have been selected for inclusion in this document and they are structural and numerical chromosomal aberrations assessed using cytogenetic methods (classical chromosomal aberration analysis (CA), fluorescence in situ hybridisation (FISH), micronuclei (MN), DNA damage (adducts, strand breaks, crosslinking, alkali-labile sites) assessed using bio-chemical/electrophoretic assays or sister chromatid exchanges (SCE); protein adducts; and hypoxanthine-
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Cyclooxygenase-independent actions of cyclooxygenase inhibitors
TL;DR: Differences in cyclooxygenase‐independent mechanisms may have consequences for the specific use of these drugs in individual patients because additional effects may either enhance the efficacy or reduce the toxicity of the respective compounds.
Journal ArticleDOI
The Transcription Factor Nuclear Factor-kappa B and Cancer
TL;DR: The role of NF- kappaB in the control of apoptosis, its link to oncogenesis, the evidence of several studies that show that NF-kappaB activation is closely related to different cancers, and finally the potential target ofNF-kappB as cancer therapy are reviewed.
Journal Article
Induced Micronucleus Frequencies in Peripheral Lymphocytes as a Screening Test for Carriers of a BRCA1 Mutation in Breast Cancer Families
Andreas Rothfuss,Petra Schütz,Sylvia Bochum,Tanja Volm,Elke Eberhardt,Rolf Kreienberg,Walther Vogel,Günter Speit +7 more
TL;DR: The results support the usefulness of induced micronucleus frequencies as a biomarker for cancer predisposition and suggest its application as a screening test for carriers of a BRCA1 mutation in breast cancer families.