Association of alkaline-phosphatase-positive reticulum cells in bone marrow with granulocytic precursors.
H Westen,D F Bainton +1 more
TLDR
It is speculated that the alkaline-phosphatase- positive reticulum cell (Al-RC) are mesenchymal stromal cells necessary for granulocytic differentiation in bone marrow.Abstract:
In the bone marrow, an elaborate stroma forms the structural basis of the hemopoietic microenvironment. In this study, two different types of stromal cells were identified with certainty on tissue sections of intact bone marrow of rats and mice using light and electron microscopic histochemistry: (a) a fibroblast-type of reticulum cell which is characterized by having alkaline phosphatase associated with its plasma membrane. We refer to this cell as the alkaline-phosphatase-positive reticulum cell (Al-RC). It is closely associated with granulocytic precursors, particularly myeloblasts and neutrophilic promyelocytes. These reticulum cells may be found throughout the marrow but are concentrated near the endosteum. (b) a macrophage-type of reticulum cell which is characterized by its abundance of lysosomal acid phosphatase and is mainly associated with erythroid precursors (as observed by others). In contrast to the above-mentioned cell type, this latter cell was found to be distributed uniformly throughout the marrow. We speculate that the Al-RC are mesenchymal stromal cells necessary for granulocytic differentiation in bone marrow.read more
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Birth and death of bone cells: basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis.
TL;DR: The role and the molecular mechanism of action of regulatory molecules, such as cytokines and hormones, in osteoclast and osteoblast birth and apoptosis are reviewed to review the evidence for the contribution of changes in bone cell birth or death to the pathogenesis of the most common forms of osteoporosis.
Journal ArticleDOI
Self-Renewing Osteoprogenitors in Bone Marrow Sinusoids Can Organize a Hematopoietic Microenvironment
Benedetto Sacchetti,Alessia Funari,Stefano Michienzi,Silvia Di Cesare,Stefania Piersanti,Isabella Saggio,Isabella Saggio,Enrico Tagliafico,Stefano Ferrari,Pamela Gehron Robey,Mara Riminucci,Paolo Bianco +11 more
TL;DR: It is shown that MCAM/CD146-expressing, subendothelial cells in human BM stroma are capable of transferring, upon transplantation, the HME to heterotopic sites, coincident with the establishment of identical subendOThelial cells within a miniature bone organ.
Journal ArticleDOI
Mesenchymal Stem Cells: Revisiting History, Concepts, and Assays
TL;DR: The experimental origin of the concept of the population(s) referred to as mesenchymal stem cells and the experimental framework required to assess their stemness and function are revisited.
Journal ArticleDOI
Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers.
James B. Mitchell,Kevin R. Mcintosh,Sanjin Zvonic,Sara M. Garrett,Z. Elizabeth Floyd,Amy Kloster,Yuan Di C. Halvorsen,Robert W. Storms,Brian C. Goh,Gail Kilroy,Xiying Wu,Jeffrey M. Gimble,Jeffrey M. Gimble +12 more
TL;DR: The adherence to plastic and subsequent expansion of human adipose‐derived cells in fetal bovine serum‐supplemented medium selects for a relatively homogeneous cell population, enriching for cells expressing a stromal immunophenotype, compared with the heterogeneity of the crude SVF.
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The chemokine receptor CXCR4 is required for the retention of B lineage and granulocytic precursors within the bone marrow microenvironment.
TL;DR: Mice reconstituted with CXCR4-deficient fetal liver cells have reduced donor-derived mature B lymphocytes in blood and lymphoid organs and the numbers of pro-B and pre-B cells are reduced in bone marrow and abnormally high in blood.
References
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Histochemistry, theoretical and applied
TL;DR: The new Pearse bids fair to become the leader, even amongst so notable a collection of books devoted entirely or largely to histochemical techniques.
Journal Article
Fibroblast precursors in normal and irradiated mouse hematopoietic organs
TL;DR: Using the in vitro colony assay, clonogenic fibroblast precursor cells (CFU-F) were detected in the bone marrow, spleen and thymus from adult mice, and the pattern of post-radiation recovery of CF U-F is similar to that of the CFU-s.
Journal ArticleDOI
Histochemical methods for acid phosphatase using hexazonium pararosanilin as coupler
Tibor Barka,Paul J. Anderson +1 more
TL;DR: ‘[he use of freslsbv diazotizecl pararosanilin w-itim a-msaphthyb pisosphate its a sinmultaneous coupling azo dye method for acid phosphatase resulted in significant improveumment because of the nmaumy desirable characteristics of the final azo dyed (1, 2).