Journal ArticleDOI
Autoradiographic localisation of α-bungarotoxin-binding sites in the central nervous system
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TLDR
Studies on the localisation of toxin-binding sites in brains would be valuable, not only to corroborate neurotoxin specificity, but also to provide information on the regional distribution of nicotinic receptor sites in the CNS.Abstract:
SNAKE α-toxins have high specificity and affinity for nicotinic receptors, and have been used widely for the characterisation of peripheral acetylcholine receptors of electric organs and skeletal muscle. In some cases autoradiography has shown the extent and local distribution of bound neurotoxins1–5. Although α-bungarotoxin (α-BTX) does not pass the blood-brain barrier and therefore does not enter the central nervous system (CNS) to a measurable extent6, central nicotinic acetylcholine receptors may bind α-toxins in vitro, and several investigators have used this toxin to characterise brain receptors7–9. Its specificity for central nicotinic receptors has been inferred not only from its peripheral action, but also from cytological and pharmacological observations: binding activity is highest in synaptosomal preparations, and is inhibited by various nicotinic drugs. Studies on the localisation of toxin-binding sites in brains, not so far reported, would be valuable, not only to corroborate neurotoxin specificity, but also to provide information on the regional distribution of nicotinic receptor sites in the CNS.read more
Citations
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Hypothalamic Integration: Organization of the Paraventricular and Supraoptic Nuclei
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A neuronal nicotinic acetylcholine receptor subunit (α7) is developmentally regulated and forms a homo-oligomeric channel blocked by α-BTX
Sabine Couturier,Daniel Bertrand,Jean-Marc Matter,Maria-Clemencia Hernandez,Sonia Bertrand,Neil S. Millar,Soledad Valera,Thomas Barkas,Marc Ballivet +8 more
TL;DR: It is concluded that alpha-bungarotoxin binding proteins in the vertebrate nervous system can function as nAChRs.
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The organization and some projections of cholinergic neurons of the mammalian forebrain.
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A new method for receptor autoradiography: [3H]opioid receptors in rat brain.
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TL;DR: The distribution of [3H]diprenorphine binding sites determined by this in vitro method is identical to the distribution found in earlier studies utilizing in vivo labeling of opioid receptors.
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Light microscopic localization of brain opiate receptors: a general autoradiographic method which preserves tissue quality
Miles Herkenham,Candace B. Pert +1 more
TL;DR: A general technique is described for using slide-mounted unfixed tissue sections to characterize and visualize drug and neurotransmitter receptors in brain or other tissues and strategies are described for determining optimal stereospecific binding with highest signal-to-noise ratios.
References
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Localization of Acetylcholine Receptor by 125I-Labeled α-Bungarotoxin Binding at Mouse Motor Endplates
TL;DR: Results indicate that the active acetylcholine receptor occupies a limited area of the junctional folds and is not distributed uniformly throughout this membrane.
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