Basfia succiniciproducens gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from bovine rumen.
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TLDR
Based on phylogenetic clustering in the MLSA analysis, the low genetic similarity to other genera and the phenotypic distinction, it is suggested to classify these bovine rumen isolates as Basfia succiniciproducens gen. nov., sp.nov.Abstract:
Gram-negative, coccoid, non-motile bacteria that are catalase-, urease- and indole-negative, facultatively anaerobic and oxidase-positive were isolated from the bovine rumen using an improved selective medium for members of the Pasteurellaceae. All strains produced significant amounts of succinic acid under anaerobic conditions with glucose as substrate. Phenotypic characterization and multilocus sequence analysis (MLSA) using 16S rRNA, rpoB, infB and recN genes were performed on seven independent isolates. All four genes showed high sequence similarity to their counterparts in the genome sequence of the patent strain MBEL55E, but less than 95 % 16S rRNA gene sequence similarity to any other species of the Pasteurellaceae. Genetically these strains form a very homogeneous group in individual as well as combined phylogenetic trees, clearly separated from other genera of the family from which they can also be separated based on phenotypic markers. Genome relatedness as deduced from the recN gene showed high interspecies similarities, but again low similarity to any of the established genera of the family. No toxicity towards bovine, human or fish cells was observed and no RTX toxin genes were detected in members of the new taxon. Based on phylogenetic clustering in the MLSA analysis, the low genetic similarity to other genera and the phenotypic distinction, we suggest to classify these bovine rumen isolates as Basfia succiniciproducens gen. nov., sp. nov. The type strain is JF4016(T) (=DSM 22022(T) =CCUG 57335(T)).read more
Citations
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Biorefineries for the production of top building block chemicals and their derivatives.
TL;DR: Current technological advances on industrial strain development for the production of platform chemicals using micro-organisms will be covered in detail with case studies on succinic acid and 3-hydroxypropionic acid as examples.
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Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase.
Jung Ho Ahn,Hogyun Seo,Hogyun Seo,Woojin Park,Jihye Seok,Jong An Lee,Won Jun Kim,Gi Bae Kim,Kyung-Jin Kim,Sang Yup Lee +9 more
TL;DR: In this article, the authors report biochemical and structural analyses of various MDHs to reveal amino acids influencing the specific activity and susceptibility to substrate inhibition, and achieve industrial-level SA production.
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Production of succinic acid by metabolically engineered microorganisms
TL;DR: In this paper, a review of metabolic engineering strategies applied for bio-based production of succinic acid using representative microorganisms, including Saccharomyces cerevisiae, Pichia kudriavzevii, Escherichia coli, Mannheimia succiniciproducts, Basfia succINiciproducens, Actinobacillus succinogenes, and Corynebacterium glutamicum, is presented.
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Towards large scale fermentative production of succinic acid.
TL;DR: Fermentative production of succinic acid from renewable carbohydrate feed-stocks can have the economic and sustainability potential to replace petroleum-based production in the future, not only for existing markets, but also new larger volume markets.
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Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen.
TL;DR: Strain 130ZT is a capnophilic, osmotolerant succinogen that utilizes a broad range of sugars and accumulates high concentrations of succinic acid, and is proposed that it be classified as a new species, Actinobacillus succ inogenes.
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Isolation and characterization of a new succinic acid-producing bacterium, Mannheimia succiniciproducens MBEL55E, from bovine rumen
TL;DR: Phylogenetic analysis based on the 16S rRNA sequence and physiological analysis indicated that the strain belongs to the recently reclassified genus Mannheimia as a novel species, and has been named MannheimIA succiniciproducens MBEL55E.
Journal ArticleDOI
Reclassification of the genus Pasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis, and Pasteurella langaa
TL;DR: The results indicated that the genus Pasteurella sensu stricto, which can be separated from the Actinobacillus group, consists of at least the following 11 species: Pasteurellaceae, with three subspecies (P. multocida, P. septica, and P. gallicida); pasteurella dagmatis sp.
Journal ArticleDOI
Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates.
TL;DR: It is concluded that the division into two species Haemophilus aphrophilus and Haemphilus paraphrophilus is not justified and that Haemophonophilus paraphphilus should be considered a later heterotypic synonym of Haemoprophilus.