Journal ArticleDOI
Biosynthesis of 50 s ribosomal subunit in Escherichia coli.
TLDR
The protein compositions and 5 s RNA contents of the nascent ribosomal particles are compared with those of protein-deficient particles obtained by exposing 50 s Ribosomal subunits to a concentrated LiCl solution and the molecular compositions are found to be similar, but not exactly the same.About:
This article is published in Journal of Molecular Biology.The article was published on 1969-03-28. It has received 66 citations till now. The article focuses on the topics: Ribosomal protein & Ribosomal RNA.read more
Citations
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Journal ArticleDOI
Specific Inactivation of 16S Ribosomal RNA Induced by Colicin E3 In Vivo
TL;DR: Results show that colicin E3 treatment causes the cleavage of 16S RNA at a specific position near the 3'-terminus, which indicates that the E3-induced defect lies solely in the 16SRNA.
Journal ArticleDOI
Assembly of bacterial ribosomes.
TL;DR: A rough approximation of the three-dimensional structure of ribosomes is likely to emerge soon and the achievement of such a complete in vitro system would represent a necessary step in the comprehensive understanding of biogenesis of organelles, and eventually, of the more complex behavior of cells.
Journal ArticleDOI
Biochemical and genetic studies on two different types of erythromycin resistant mutants of Escherichia coli with altered ribosomal proteins.
H. G. Wittmann,Georg Stöffler,David Apirion,L. Rosen,Kentaro Tanaka,Mikio Tamaki,Renkichi Takata,Satoshi Dekio,Eiko Otaka +8 more
TL;DR: Transduction experiments showed that the mutations affecting both proteins, L4 and L22, are located very close to the str and spc genes, nearer to the spc than to str gene.
Journal ArticleDOI
Intermediates and time kinetics of the in vivo assembly of Escherichia coli ribosomes
TL;DR: In the kinetic experiments the specific activity of the nucleoside triphosphates and of the different ribosomal particles was followed after addition of a radioactive RNA precursor to the growth medium, and a digital simulation of the flow of isotopes through the assembly pathways indicated that approximately 2% of the total 23 S type RNA, as well as 2%of the total 16 S typeRNA, is contained in the precursor particles.
References
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Journal ArticleDOI
Molecular characterization of ribonucleic acid from Escherichia coli ribosomes
TL;DR: The ribon nucleic acid from purified 30 s, 50 s and 70 s ribosomes of E. coli has been isolated by the phenol and detergent methods and it is suggested that they are heterogeneous; some contain one 23 s ribonucleic acid component, while others contain two 16 s units.
Journal ArticleDOI
Nucleotide sequence of 5S-ribosomal RNA from Escherichia coli.
TL;DR: Using a two-dimensional fractionation procedure for the separation of nucleotides labelled with phosphorus-32, the sequences of all the oligonucleotides obtained by complete digestion of 32P-labelled 5S RNA are determined and the unique sequence shown in Figs.
Journal ArticleDOI
Biosynthetic precursors of 30S and 50S ribosomal particles in Escherichia coli.
Journal ArticleDOI
Distribution of 5 s RNA in HeLa cells.
Ernest Knight,James Darnell +1 more
TL;DR: Kinetic measurements of the relative labeling of 5 and 28 s RNA with radioactive uridine suggest a pool of 5 s RNA in the HeLa cell nucleus, and direct measurement of the5 s RNA content of the nucleus indicates that the pool contains 20 to 25% of the total 5 sRNA in the SheLa cell.
Journal ArticleDOI
Synthesis and accumulation of low molecular weight RNA during embryogenesis of Xenopus laevis.
Donald D. Brown,Elizabeth Littna +1 more
TL;DR: The high content of 4 s RNA in the unfertilized egg reflects the remarkably high synthetic rate of ribosomal RNA which occurs during oogenesis, and the amount of 5 s RNA per ribosome remains constant throughout early development, and there is no detectable synthesis of5 s RNA at early stages.
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