Journal ArticleDOI
Biosynthesis of halogenated metabolites by bacteria
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TLDR
Haloperoxidases, enzymes capable of catalyzing the formation of carbon halogen bonds in the presence of hydrogen peroxide and halide ions have been isolated and characterized from different bacteria, but these enzymes turned out to be very unspecific and are obviously not the type of halogenating enzymes responsible for theformation of halometabolites in bacteria.Abstract:
Halogenated metabolites, originally thought to be infrequent in nature, are actually nothing unusual at all, and are produced by many different organisms, including bacteria. Whereas marine bacteria usually produce brominated compounds, terrestrial bacteria preferentially synthesize chlorometabolites, but fluoro- and iodometabolites can also be found. Haloperoxidases, enzymes capable of catalyzing the formation of carbon halogen bonds in the presence of hydrogen peroxide and halide ions (Cl-, Br- and I-) have been isolated and characterized from different bacteria. These enzymes turned out to be very unspecific and are obviously not the type of halogenating enzymes responsible for the formation of halometabolites in bacteria. A yet-unknown type of halogenating enzyme having both substrate and regio-specificity must be involved in the biosynthesis of halogenated compounds.read more
Citations
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The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti
Turlough M. Finan,Stefan Weidner,Kim Wong,Jens Buhrmester,Patrick S. G. Chain,Frank J. Vorhölter,Ismael Hernández-Lucas,Anke Becker,Alison Cowie,Jérôme Gouzy,Brian Golding,Alfred Pühler +11 more
TL;DR: Analysis of the 1,683,333-nt sequence of the pSymB megaplasmid from the symbiotic N2-fixing bacterium Sinorhizobium meliloti revealed that the replicon has a high gene density with a total of 1,570 protein-coding regions, with few insertion elements and regions duplicated elsewhere in the genome.
Journal ArticleDOI
Halogenation Strategies In Natural Product Biosynthesis
TL;DR: The current state of knowledge regarding the mechanisms of these transformations are reviewed, applications of this knowledge are highlighted, and future opportunities and challenges for the field are proposed.
Journal ArticleDOI
Characterization of the Pyoluteorin Biosynthetic Gene Cluster of Pseudomonas fluorescens Pf-5
TL;DR: Ten genes required for the biosynthesis of pyoluteorin, an antifungal compound composed of a bichlorinated pyrrole linked to a resorcinol moiety, were identified within a 24-kb genomic region of Pseudomonas fluorescens Pf-5 and deduced amino acid sequences were similar to those of members of the LysR family of transcriptional activators.
Journal ArticleDOI
Natural products with antifungal activity from Pseudomonas biocontrol bacteria
James M. Ligon,D. S. Hill,Philip E. Hammer,N. R. Torkewitz,Dirk Hofmann,Hans‐Joachim Kempf,Karl-Heinz van Pée +6 more
TL;DR: These studies suggest that the biocontrol activity of bacteria can be dramatically increased to rival the activity of commercial chemical fungicides through directed genetic modification.
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Crystal structure and mechanism of a bacterial fluorinating enzyme
Changjiang Dong,Fanglu Huang,Hai Deng,Christoph Schaffrath,Jonathan B. Spencer,David O'Hagan,James H. Naismith +6 more
TL;DR: The sequence and three-dimensional structure of the first native fluorination enzyme, 5′-fluoro-5′-deoxyadenosine synthase, from Streptomyces cattleya is reported, and a nucleophilic substitution mechanism for this biological fluorination reaction is proposed.
References
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Journal ArticleDOI
Chloroperoxidase I. ISOLATION AND PROPERTIES OF THE CRYSTALLINE GLYCOPROTEIN
David R. Morris,Lowell P. Hager +1 more
TL;DR: Chloroperoxidase from Caldariomyces fumago has been isolated in crystalline form and Chromatography of acid hydrolysates reveals that glucosamine and arabinose are major constituents.
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Chloroperoxidase. II. Utilization of halogen anions.
TL;DR: Results indicate that fluoride ion can compete for both the hydrogen peroxide- and the halogen anion-binding sites on chloroperoxidase.
Journal ArticleDOI
Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications.
Susanne Fetzner,Franz Lingens +1 more
TL;DR: This review is a survey of bacterial dehalogenases that catalyze the cleavage of halogen substituents from haloaromatics, haloalkanes, h Haloalcohols, and h Haloalkanoic acids.
Journal ArticleDOI
Global control in Pseudomonas fluorescens mediating antibiotic synthesis and suppression of black root rot of tobacco.
Jacques Laville,Christophe Voisard,Christoph Keel,Monika Maurhofer,Geneviève Défago,Dieter Haas +5 more
TL;DR: The gacA mutants of strain CHA0 have a drastically reduced ability to suppress black root rot under gnotobiotic conditions, supporting the previous observations that the antibiotic Phl and HCN individually contribute to the suppression of blackroot rot.