Bovine Embryo Culture in the Presence or Absence of Serum: Implications for Blastocyst Development, Cryotolerance, and Messenger RNA Expression
Dimitrios Rizos,Alfonso Gutiérrez-Adán,S. S. Pérez-Garnelo,J. de la Fuente,Maurice P. Boland,Patrick Lonergan +5 more
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TLDR
It is shown that conditions of postfertilization culture, in particular, the presence of serum in the medium, can affect the speed of embryo development and the quality of the resulting blastocysts.Abstract:
We have previously shown that, while the intrinsic quality of the oocyte is the main factor affecting blastocyst yield during bovine embryo development in vitro, the main factor affecting the quality of the blastocyst is the postfertilization culture conditions. Therefore, any improvement in the quality of blastocysts produced in vitro is likely to derive from the modification of the postfertilization culture conditions. The objective of this study was to examine the effect of the presence or absence of serum and the concentration of BSA during the period of embryo culture in vitro on 1) cleavage rate, 2) the kinetics of embryo development, 3) blastocyst yield, and 4) blastocyst quality, as assessed by cryotolerance and gene expression patterns. The quantification of all gene transcripts was carried out by real-time quantitative reverse transcription-polymerase chain reaction. Bovine blastocysts from four sources were used: 1) in vitro culture in synthetic oviduct fluid (SOF) supplemented with 3 ...read more
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Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification
Joseph Saragusty,Amir Arav +1 more
TL;DR: The major strides and obstacles in human and other vertebrate oocyte and embryo cryopreservation will be reviewed here.
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Long-term effect of in vitro culture of mouse embryos with serum on mRNA expression of imprinting genes, development, and behavior
Raúl Fernández-González,Pedro Moreira,Ainhoa Bilbao,Adela Jiménez,Miriam Pérez-Crespo,Miguel Ángel Ramírez,Fernando Rodríguez de Fonseca,Belén Pintado,Alfonso Gutiérrez-Adán +8 more
TL;DR: Mice derived from embryos cultured with FCS exhibited specific behavioral alterations in anxiety and displayed deficiencies in implicit memories, indicating that long-term programming of postnatal development, growth, and physiology can be affected irreversibly during the preimplantation period of embryo development by suboptimal in vitro culture.
Journal ArticleDOI
Oocyte and embryo quality: effect of origin, culture conditions and gene expression patterns.
TL;DR: Evidence for an effect of oocyte origin and/or in vitro maturation conditions on the developmental capacity and gene expression patterns in the oocyte is demonstrated and the well-documented effects of post-fertilization culture environment on embryo gene expression and quality are highlighted.
Journal ArticleDOI
Temporal Divergence in the Pattern of Messenger RNA Expression in Bovine Embryos Cultured from the Zygote to Blastocyst Stage In Vitro or In Vivo
Patrick Lonergan,Dimitrios Rizos,Alfonso Gutiérrez-Adán,P. M. Moreira,Belén Pintado,J. de la Fuente,Maurice P. Boland +6 more
TL;DR: The results demonstrate that the relative abundance of the transcripts studied varies throughout the preimplantation period and is strongly influenced by the culture environment, and changes in transcript abundance in blastocyst stage embryos are in many cases a consequence of perturbed transcription earlier in development.
Journal ArticleDOI
Large-scale transcriptional analysis of bovine embryo biopsies in relation to pregnancy success after transfer to recipients.
Ashraf M. El-Sayed,Michael Hoelker,Franca Rings,Dessie Salilew,Danyel Jennen,Ernst Tholen,Marc-André Sirard,Karl Schellander,Dawit Tesfaye +8 more
TL;DR: Direct candidates of blastocyst-specific genes which may play an important role in determining the fate of the embryo after transfer are generated.
References
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TL;DR: It is now common to add antioxidant compounds to culture media, but maintaining the pro-oxidant-antioxidant equilibrium in embryos through such supplementation is a complex problem.
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TL;DR: The results indicate that the intrinsic quality of the oocyte is the main factor affecting blastocyst yields, while the conditions of embryo culture have a crucial role in determining Blastocyst quality.