Book ChapterDOI
Calbindin D28K Gene Expression in Neurodegenerative Diseases
TLDR
Analysis of the role that changes in calbindin D 28K gene expression may play in the etiology and pathogenesis of neurodegeneration in the brain, such as Huntington's, Parkinson's, and Alzheimer's diseases revealed that in each disease entity, the brain area that is particularly affected and used as a hallmark for the neuropathological changes exhibits a significant reduction in cal bindin D 27K geneexpression.Abstract:
Publisher Summary This chapter focuses on calbindin D 28K gene expression in aging and neurodegenerative diseases. The study described in the chapter has permitted an analysis of the role that changes in calbindin D 28K gene expression may play in the etiology and pathogenesis of neurodegeneration in the brain, such as Huntington's, Parkinson's, and Alzheimer's diseases. A method of molecular cloning of calbindin D 28K described in the chapter resulted in the isolation of a cloned calbindin D 28K cDNA via direct immunological screening of a mouse cerebellar λgt11 bacterial expression library. The calbindin D 28K cDNA could then be radiolabeled directly and utilized as a probe for detection of calbindin D 28K mRNA (slot-blot and Northern blot analyses) or converted to radiolabeled cRNA transcripts to detect calbindin D 28K mRNA at the cellular level by in situ hybridization. Slot-blot and Northern blot hybridization analyses revealed that in each disease entity, the brain area that is particularly affected and used as a hallmark for the neuropathological changes exhibits a significant reduction in calbindin D 28K gene expression.read more
Citations
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Journal ArticleDOI
Calbindins decreased after space flight
TL;DR: Findings after a short-term exposure to microgravity or modeled weightlessness suggest that a decreased expression of calbindins may contribute to the disorders of Ca2+ metabolism induced by space flight.
Dissertation
Vitamin D, light exposure, sleep and musculoskeletal health in South Asian and Caucasian women: biological and social influences
TL;DR: It is suggested that older South Asian women are in need of intervention to improve vitamin D status, and there is also some evidence for poorer musculoskeletal health, lower light exposure and poorer sleep in this group.
Functional studies of Calbindin-D28K and its role in intracellular calcium homeostasis
TL;DR: Examination of Ca -buffering by CaBP in stably transfected H E K 293 and HeLa cell lines provides unequivocal evidence that CaBP can act to buffer increases in intracellular Ca 2 + .
Vitamin D endocrine system after short-term space flight
TL;DR: In this paper, the effects of microgravity on calbindins in rats in space vs. "grounded" animals (synchronous Animal Enclosure Module controls and tail suspension controls) were compared.
References
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Journal Article
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Journal ArticleDOI
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