BookDOI
Calcium signaling protocols
TLDR
Continuous Fluorescent Monitoring of Cellular Calcium Fluxes: A Novel Perfusion System for the Investigation of Inosito(1,4,5)trisphosphate-Dependent Quantal Calcium Release Using Immobilized, Electropermeabilized Cells, Robert A. Wilcox and James Strupish.Abstract:
Part I. General. Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations, Alec W. M. Simpson. Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2, Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. Lambert. Measurement of [Ca2+]i in Cell Suspensions Using Indo-1, Adriaan Nelemans. Part II. Specialist Measurement Systems. Confocal Microscopy: Theory and Applications, Laura C. Mongan, Joanne Gormally, Andrew R. D. Hubbard, Christopher d'Lacey, and Colin D. Ockleford. Measurement of Intracellular Calcium Concentration Using Confocal Microscopy, Carmen Perez-Terzic, Marisa Jaconi, and Lisa Stehno-Bittel. Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i), Anthony J. Morgan and Andrew P. Thomas. Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR), Elaine Sullivan, Emily M. Tucker, and Ian L. Dale. Part III. Nonelectrophysiological Measurement of Ca2+ Channel Activity. Measurement of Ca2+ Entry Using 45Ca2+, Mercedes Villarroya, Manuela G. Lopez, Maria F. Cano-Abad, and Antonio G. Garcia. Measurement of [3H]PN200-110 and [125I]w-Contoxin MVIIA Binding, Kazuyoshi Hirota and David G. Lambert. Part IV. Measurement of Ins(1,4,5)P3 and Ca2+ Release From Intracellular Stores. Measurement of Inositol (Poly)phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells, Philip Swigart and Shamshad Cockcroft. Measurement of Inositol(1,4,5)triphosphate Using a Stereospecific Radioreceptor Mass Assay, Darren Smart. Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay, Robert A. Wilcox. Microinjection of myo-Inositol(1,4,5)triphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells, Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann. Photolysis of Caged Calcium Using a Low-Cost Flash Unit, Gommert A. van Koeveringe and Ron van Mastrigt. Measurement of Ca2+ Flux Through Ins(1,4,5)P3 Receptor - Ca2+ Channels in Lipid Bilayers ("Dip-Tip" and "Schindler" Methodology), Edwin C. Thrower. Continuous Fluorescent Monitoring of Cellular Calcium Fluxes: A Novel Perfusion System for the Investigation of Inosito(1,4,5)trisphosphate-Dependent Quantal Calcium Release Using Immobilized, Electropermeabilized Cells, Robert A. Wilcox and James Strupish. Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators, Aldebaran M. Hofer. Part V. Specialist Measurement Techniques. Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry, Hideo Kanaide. Measurement of Calcium and Movement in Heart Cells, Leong L. Ng and Paulene A. Quinn. Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations, Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, and Ronald M. Lynch. Measurement of Cytosolic-Free Ca2+ in Plant Tissue, Martin R. McAinsh and Irina Staxen. Part VI. Ca2+ Sensitive Targets. Assay and Purification of Calmodulin-Dependent Protein Kinase, Rakesh Kakkar and Rajendra K. Sharma. Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1), Danuta Kosk-Kosicka. Index.read more
Citations
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Journal ArticleDOI
Bcl-2 functionally interacts with inositol 1,4,5-trisphosphate receptors to regulate calcium release from the ER in response to inositol 1,4,5-trisphosphate
Rui-rui Chen,Ignacio Valencia,Fei Zhong,Karen S. McColl,H. Llewelyn Roderick,Martin D. Bootman,Michael J. Berridge,Stuart J. Conway,Andrew B. Holmes,Gregory A. Mignery,Patricio Velez,Clark W. Distelhorst +11 more
TL;DR: The anti-apoptotic protein Bcl-2 significantly inhibited InsP3-mediated calcium release and elevation of cytosolic calcium in WEHI7.2 T cells because responses to both anti-CD3 antibody and a cell-permeant InsP 3 ester were decreased.
Patent
Boron-containing small molecules
Stephen J. Baker,Tsutomu Akama,Carolyn Bellinger-Kawahara,Vincent Hernandez,Karin M. Hold,James Leyden,Kirk R. Maples,Jacob J. Plattner,Virginia Sanders,Yong-Kang Zhang +9 more
TL;DR: In this paper, the authors present compounds that have physiochemical properties that facilitate penetration of the nail plate and have properties that allow the compound to reach the particular part of the skin, nail, hair, claw or hoof infected by the fungus.
Journal ArticleDOI
Pharmacological characterisation of human 5-HT2 receptor subtypes.
Jeffrey C. Jerman,Stephen J. Brough,Tracey Gager,Martyn D. Wood,Martyn C. Coldwell,Darren Smart,Derek N. Middlemiss +6 more
TL;DR: The present study provides a direct comparison of agonist and antagonist pharmacology at 5-HT(2) receptor subtypes in a homogenous system and confirms that agonist potency and efficacy varies with the level of receptor expression.
Journal ArticleDOI
Antiapoptotic signaling by a remyelination-promoting human antimyelin antibody.
TL;DR: It is concluded that rHIgM22 may induce remyelination via rescue of oligodendrocytes, and it is suggested that such autoantibody-mediated signaling may have important therapeutic implications for a variety of neurological diseases, including stroke and Alzheimer's disease.
Journal ArticleDOI
Urantide mimics urotensin-II induced calcium release in cells expressing recombinant UT receptors
Valeria Camarda,Wei Song,Erika Marzola,Martina Spagnol,Remo Guerrini,Severo Salvadori,Domenico Regoli,Jonathan P. Thompson,David J. Rowbotham,David J. Behm,Stephen A. Douglas,Girolamo Calo,David G. Lambert +12 more
TL;DR: Urantide behaves as a competitive, potent, and pure antagonist in the rat aorta bioassay, while as an agonist in a calcium mobilization assay performed in CHO(hUT) cells and should be considered a low efficacy partial agonist.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Book
Antibodies: A Laboratory Manual
Ed Harlow,David P. Lane +1 more
TL;DR: A second edition of Antibodies: A Laboratory Manual is being published in September 2013, Revised, extended and updated by Edward Greenfield of the Dana-Farber Cancer Center, the material has been recast with extensive new information and new chapters have been added.
Journal ArticleDOI
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
TL;DR: A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+ using an 8-coordinate tetracarboxylate chelating site with stilbene chromophores that offer up to 30-fold brighter fluorescence.
Journal ArticleDOI
The Attractions of Proteins for Small Molecules and Ions
TL;DR: The number and variety of known compounrjs between proteins and small molecules are increasing rapidly and make a fascinating story as discussed by the authors, and there are many compounds of serum albumin, which was used during the war by many chemists, most of whom found at least one 6ew compound.